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A comprehensive analysis of carbon flow to the tricarboxylic acid cycle proves the necessity of a flux alternative to glycolysis and phosphoenolpyruvate carboxylase activity for the dark CO2 fixation reaction. Only two separate carbon fluxes can supply the cycle with enough carbon skeleton for biosyntheses starting from both C4 and C5acids, and especially for protein amino acid production. It is postulated that malonate is the CO2 acceptor alternative to phosphoenolpyruvate, and that it originates from glucose through gluconate and glycerate. This pathway would explain the metabolism of malonate and the substantial amount of this substance found in legumes, as well as the role of the malic enzyme.

Affiliations: 1: Department of Biochemistry, Institute of Biology, A. Mickiewicz University


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