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In agar cultures, the morphogenic pattern of Nephrolepis exaltata cv. Bostoniensis shoot tips is directed mainly toward leaf unfolding and growth. Submerged shoot tips or meristemoids in liquid cultures produced mostly meristemoid clusters, with limited leaf development. Aeration in liquid cultures, in rotated flasks, or in bubble bioreactors, neither enhanced leaf formation nor inhibited meristemoid development. Higher levels of agar in the medium resulted in a smaller number of meristemoids and a larger number of leaves developing on meristemoid aggregates initiated in liquid medium. Large-scale liquid cultures provided an efficient method for the production of meristemoid clusters in N. exaltata cv. Bostoniensis, which were then separated mechanically and developed into normal plants.

Affiliations: 1: Department of Agricultural Botany and The Otto Warburg Center for Biotechnology in Agriculture, The Hebrew University of Jerusalem, Faculty of Agriculture


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1. Ammirato P.V., Styer D.J.,"Strategies for large-scale manipulation of somatic embryos in suspension cultures"Biotechnology in Plant Science. Zaitlin M.Academic PressNew York1985161178
2. Cooke R.C."Homogenation as an aid in tissue culture propagation of Platycerum, Davalia"HortScience1979Vol 142122
3. Debergh P.C., Harbaoui Y., Lemeur R."Mass propagation of globe artichoke (Cynara scolymus), evaluation of different hypotheses to overcome vitrification with special reference to water potential."Physiol. Plant.1981Vol 53181187
4. George E.F., Sherrington P.D."10"Plant Propagation by Tissue Culture.1984Chapter. Exegetics Ltd., Hants
5. Higuchi H., Amaki W."Effects of 6-benzylaminopurine on the organogenesis of Asplenium nidus L. through in vitro propagation"Sci. Hortic. (Amsterdam)1989Vol 37351359
6. Higuchi H., Amaki W., Suzuki S."In vitro propagation of Nephrolepis cordiflora"Sci. Hortic. (Amsterdam)1987Vol 32105113
7. Kessel R.H., Carr A.A."The effect of dissolved oxygen concentration on growth and differentiation of carrot (Daucus carota) tissue."J. Exp. Bot.1972Vol 239961007
8. Knauss J.F."A partial tissue culture method for pathogen-free propagation of selected ferns from spores."Proc. Ra. State Hortic. Sci.1976Vol 89363365
9. Le C.E."Essai de multiplication de Nephrolepis exaltata par culture in vitro de tissu gamtophytique."Rev. Suisse Vitic. Arboric. Hortic.Vol 15189192
10. Leffering L., Soede A.C.,"Influence of salt concentration on the homogeneity of Nephrolepis plants from tissue culture."Plant Tissue Culture 1982. Abe Printing Co., Tokyo Fujiwara A.1982499500
11. Levin R., Gaba V., Tal B., Hirsch S., Denola D., Vasil I.K."Automated plant tissue culture for mass propagation."Bio Technology1988Vol 610351040
12. Loescher W.H., Albrecht C.N."Development in vitro of Nephrolepis exaltata Bostoniensis runner tissue."Physiol. Plant.1979Vol 47250254
13. Murashige T."Plant cell and organ culture as horticultural practices."Acta Hortic.1977Vol 781730
14. Murashige T., Skoog F."A revised medium for rapid growth and bioassays with tobacco tissue culture."Physiol. Plant.1962Vol 15473497
15. Preil W., Florek P., Wix U., Beck A."Towards mass propagation by use of bioreactors."Acta Hortic.1988Vol 22699106
16. Richards J.H., Beck J.Z., Hirsch A.M."Structural investigation of asexual reproduction in Nephrolepis exaltata, Platycerum bifurcatum"Am. J. Bot.1983Vol 709931001
17. Sluis E.F., Walker K.A."Commercialization of plant tissue culture propagation."IATPC Newsletter1985Vol 47212
18. Steward F.C., Shantz E.M.,"The chemical induction of growth in plant tissue culture"The Chemistry and Mode of Action of Plant Growth Substances. Wain R.L., Wigntman F.ButterworthsLondon1956165186
19. Styer D.J.,"Bioreactor technology for plant propagation"Tissue Culture in Forestry and Agriculture. Henke R.R.PlenumNew York1985117130
20. Takayama S., Misawa M."Mass-propagation of Begonia hiemalis plantlets by shake culture."Plant Cell Physiol.1981Vol 22461467
21. Takayama S., Misawa M."The mass-propagation of Lilium in vitro by stimulation of multiple adventitious bulb-scale formation and by shake culture."Can. J. Bot.1983Vol 61224228
22. Ziv M.,"In vitro hardening and acclimatization of tissue culture plants"Plant Tissue Culture and Its Agricultural Applications. Withers L.A., Alderson P.G.ButterworthsLondon1986187196

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