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EFFECT OF GROWTH REGULATORS AND CULTURE ENVIRONMENT ON SOMATIC EMBRYOGENESIS OF ACACIA CATECHU WILLD

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Somatic embryogenesis was achieved from callus derived from immature cotyledons of Acacia catechu Willd on Woody Plant Medium (WPM) supplemented with 13.9 μM kinetin and 2.7 μM α-naphthaleneacetic acid. Somatic embryos formed when the calli were grown for 2 weeks in the dark followed by incubation in the light with a 16-h photoperiod. Embryogenesis did not occur with continuous incubation in either the dark or the light. The addition of L-proline (0.87–5.21 mM) to the medium promoted the development of somatic embryos and induced secondary somatic embryogenesis. The light-green somatic embryos germinated on half-strength Murashige and Skoog (MS) medium supplemented with 2% (w/v) sucrose devoid of growth regulators. Somatic embryos germinated into plantlets which were hardened in the greenhouse and subsequently transferred to the field.

Affiliations: 1: Plant Tissue Culture Laboratory, Regional Plant Resource Centre

10.1080/07929978.1995.10676611
/content/journals/10.1080/07929978.1995.10676611
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/content/journals/10.1080/07929978.1995.10676611
1995-05-13
2018-06-19

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