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MICROPROPAGATION AND IN VITRO STORAGE OF CENTAURIUM RIGUALII ESTEVE (GENTIANACEAE)

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Centaurium rigualii Esteve, an endangered plant species from southeastern Spain with potentially useful secondary products, was successfully micropropagated from shoot tips using MS supplemented with 0.44 μM 6- benzylaminopurine (BA) and 0.05 μM 1-naphthaleneacetic acid (NAA) at the proliferation step, and MS medium alone or with varying concentrations of indole-3-butyric acid (IBA) at the rooting step. No incidence of somaclonal variation was detected among randomly chosen shoots from ten subclonal lines in eight isozyme systems studied and in chromosome counts. However, this does not preclude the existence of somaclonal variation at other levels of the genome. A 90% survival rate was obtained when shoots were stored for three years in MS medium supplemented with 4.92 μM IBA at 5 °C and with a 16-h photoperiod.

Affiliations: 1: Departamento de Biología Vegetal, Escuela T.S. de Ingenieros Agrónomos, Universidad Politécnica de Madrid

10.1080/07929978.1996.10676640
/content/journals/10.1080/07929978.1996.10676640
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/content/journals/10.1080/07929978.1996.10676640
1996-05-13
2018-06-18

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