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A PROTOCOL FOR HORSEGRAM [MACROTYLOMA UNIFLORUM (LAM.) VERDC.] CALLUS INDUCTION

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Calli of Macrotyloma uniflorum (Lam.)Verdc.) Co.1 and Bgm.1 were established from expiants of epicotyl, hypocotyl, cotyledon, and young leaf on MS medium supplemented with auxins and cytokinins (2, 4-D, IAA, NAA, BAP, KN, and CM). When they were cultured subsequently on various combinations and different concentrations, BAP and 2,4-D were found to yield green compact callus in all expiants. Yellow friable calli were obtained on MS medium supplemented with auxins (2,4-D or NAA or IAA). Browning of callus was observed at a higher concentration of (2.5 mg1−1) auxins after 25 days.

Affiliations: 1: Mutation Breeding and Plant Tissue Culture Unit, Department of Plant Science, School of Life Sciences, Bharathidasan University

10.1080/07929978.1996.10676643
/content/journals/10.1080/07929978.1996.10676643
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/content/journals/10.1080/07929978.1996.10676643
1996-05-13
2018-09-19

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