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Incompatibility between the species Nicotiana gossey Domin. and N. tabacum L., resulting in complete sterility of F1 hybrid, was overcome using in vitro techniques. Explants of stem parenchyma were put in culture and were regularly subcultured on Murashige and Skoog basal medium, supplemented with different compounds: α-naphthaleneacetic acid (2 mg L−1) and kinetin (0.5 mg L−1) for callus induction, kinetin (2 mg L−1) and indule-3-acetic acid (0.5 mg L−1) for organ formation, and ferulic acid (2 mg L−1) for rooting. The regenerants obtained showed significant morphological and cytological variability. They differed from each other as well as from the initial material in stem length, leaf size, and flower morphology. Most of the plants examined were mixoploids. A great number of aberrations were established during the meiotic division of the regenerants. Pollen viability varied from 0 to 92%. The results of our investigations showed that the tissue culture method could be successfully applied not only for overcoming species incompatibility but also for inducing somaclonal variation and creation of a large variety of plant forms.

Affiliations: 1: Institute of Genetics, BAS ; 2: Institute of Genetics, BAS


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1. D'Amato F.,"Chromosome number variation in cultured cells and regenerated plants"Frontiers of plant tissue culture, Univ. of Calgary Press, Calgary, Alberta, Canada Thorpe T.A.1978287295
2. D'Amato F."Nuclear changes in cultured plant cells."Caryologia1991Vol 44217224
3. Burk L.G., Heggestad H.E."The genus Nicotiana: a source of resistance to diseases of cultivated tobacco."Econ. Bot.1966Vol 207688
4. Gerstel D."Cytoplasmic male sterility in Nicotiana (a review). North Carolina Agrie. Exp. Sta. Tech. Bull. No. 263"198031
5. Kao K.N., Miller R.H., Gamborg O.L."Variations in chromosome number and structure in plant cells grown in suspension cultures."Can. J. Genet. Cytol.1970Vol 12297
6. Kaul M.,"Male sterility in higher plants"Monographs on theoretical and applied genetics. Frankel R., Grossman M., Maliga P.Springer-VerlagBerlin1988Vol 10775795
7. Krishnamurthy M.,"Sugarcane improvement through tissue culture and review of progress"Tissue culture of economically important plants. Asian Network for Biological Sciences, Singapore, 70 Rao A.N.1981
8. Litz R.E."Organogenesis and somatic embryogenesis."Acta Hort.1993Vol 336199205
9. Murashige T., Nakano R."Tissue culture as a potential tool in obtaining polyploid plants."J. Hered.1966Vol 57115
10. Murashige T., Skoog F."A revised medium for rapid growth and bioassays with tobacco tissue cultures."Physiol. Plant.1962Vol 15473497
11. Nikova V.M."Diagram of determining the types of change of the flowers in the various sources of cytoplasmic male sterility in Nicotiana"C. R. Acad. Bulg. Sci.1984Vol 3711151118
12. Nikova V.M., Zagorska N.A."Variability of regenerants obtained in tissue culture of Nicotiana velutina Wheeler, x N. tabacum L. Proc. IIId Natl. Conf. Cytogenetics, Plovdiv, Bulgaria"1984Vol 1191194
13. Nikova V.M., Zagorska N.A."Overcoming hybrid incompatibility between Nicotiana africana Merxm. and N. tabacum L. and development of cytoplasmically male sterile tobacco forms"Plant Cell, Tissue Org. Cult.1990Vol 237175
14. Nikova V.M., Zagorska N.A., Pundeva R."Development of four tobacco cytoplasmic male sterile sources using in vitro techniques."Plant Cell, Tissue Org. Cult.1991Vol 27289295
15. Orton T.Y."A quantitative analysis of growth and regeneration from tissue cultures of Hordeum vulgare, H. jubatum, and their interspecific hybrid."Environ. Exp. Bot.1979Vol 19319335
16. Orton T.J."Experimental approaches to the study of somaclonal variation."Plant Mol. Biol. Rep.1983Vol 167
17. Singh B.D., Harvey B.L."Does 2,4-D induce mitotic irregularities in plant tissue culture?"Experientia1975Vol 32785
18. Skirvin R.M., Norton M., McPheeters K.D."Somaclonal variation: has it proved useful for plant improvement?"Acta Hort.1993Vol 336333340
19. Skirvin R.M., McPheeters K.D., Norton M."Sources and frequency of somaclonal variation."Hort. Sci.1994Vol 2912321237
20. Ternovsky M.F."Interspecific hybridization in the genus Nicotiana. Nat. Inst. Tobacco, Krasnodar, USSR"1958275- 281 (in Russian)
21. Thurston R."Resistance in Nicotiana to the green peach aphid and some other insects."J. Econ. Entomol.1961Vol 54946- 949
22. Wenzel G., Schieder O., Przeworny T., Sopory S., Melchers G."Comparison of single cell culture derived Solanum tuberosum L. plants and a model for their application in breeding programs"Theor. Appl. Genet.1979Vol 5549

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