Cookies Policy
X

This site uses cookies. By continuing to browse the site you are agreeing to our use of cookies.

I accept this policy

Find out more here

Study on the expression of specific chimeric-orfs of C type cytoplasmic male sterile in maize

No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
The full text of this article is not currently available.

Brill’s MyBook program is exclusively available on BrillOnline Books and Journals. Students and scholars affiliated with an institution that has purchased a Brill E-Book on the BrillOnline platform automatically have access to the MyBook option for the title(s) acquired by the Library. Brill MyBook is a print-on-demand paperback copy which is sold at a favorably uniform low price.

Access this article

+ Tax (if applicable)
Add to Favorites
You must be logged in to use this functionality

image of Israel Journal of Plant Sciences

In the present study, a mutated chimeric orf364, named orf366-c, was identified in a maize C type cytoplasmic male sterile line (CMS-C) by PCR and RT-PCR. Four base pair adjacent base substitution mutations (CAAA to TTTT) and eight bp insertion mutations were found in orf366-c compared with orf364. ORF366-C was predicted to contain one membrane-spanning domain using TMHMM online software. Real-time quantitative PCR analysis showed that orf366-c was upregulated in the CMS-C line in comparison to its maintainer line at the uninucleate stage. The protokaryotic protein expression of orf366-c in Escherichia coli showed that ORF366-C could be a cytotoxic protein. All the results indicated that orf366-c may be associated with maize CMS-C.

Affiliations: 1: Maize Research Institute of Sichuan Agricultural University; Key Laboratory of Crop Genetic Resource and Improvement, Ministry of Education; Key Laboratory of Maize Biology and Genetic Breeding on Southwest Ministry of Agriculture ; 2: College of Life Science of Sichuan Normal University

10.1080/07929978.2014.945314
/content/journals/10.1080/07929978.2014.945314
dcterms_title,pub_keyword,dcterms_description,pub_author
10
5
Loading
Loading

Full text loading...

/content/journals/10.1080/07929978.2014.945314
Loading

Data & Media loading...

http://brill.metastore.ingenta.com/content/journals/10.1080/07929978.2014.945314
Loading

Article metrics loading...

/content/journals/10.1080/07929978.2014.945314
2013-05-18
2018-06-18

Sign-in

Can't access your account?
  • Tools

  • Add to Favorites
  • Printable version
  • Email this page
  • Subscribe to ToC alert
  • Get permissions
  • Recommend to your library

    You must fill out fields marked with: *

    Librarian details
    Your details
    Why are you recommending this title?
    Select reason:
     
    Israel Journal of Plant Sciences — Recommend this title to your library
  • Export citations
  • Key

  • Full access
  • Open Access
  • Partial/No accessInformation