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Comparison of the protein profile of the reproductive system between bisexual and parthenogenetic species of Artemia (Branchiopoda, Anostraca)

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In the present study we investigated the differences in protein profile of the reproductive systems of two species of Iranian Artemia, i.e., Artemia urmiana and Artemia parthenogenetica. The reproductive systems (ovary and uterus filled with cysts) of adult females of Artemia were dissected and kept at −70°C. These tissues were used for protein extraction and the protein solution was assayed by the Bradford test to evaluate the quantity of the protein. Proteins were next exposed to two-dimensional gel electrophoresis and the protein spots obtained from the gels were compared between the two species. Through a comparative proteomic approach, the protein profiles of the ovary and the uterus with cysts from the two species of Artemia were analysed. Among the spots obtained, 21 differentially expressed proteins were recognized. Following mass spectrometry, three of these proteins, 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, the BRCA1 (breast and ovarian susceptibility protein 1) associated RING domain (BARD1) protein, and tubulin β3, were found to be expressed differentially between the two species. Also some other proteins, with unknown functions, were found to be over-expressed in the A. parthenogenetica gonads. Dans ce travail, nous avons analysé les différences entre les profils en protéine du système reproducteur de deux espèces iraniennes d'Artémies, Artemia urmiana et Artemia parthenogenetica. Le système reproducteur (ovaires et utérus avec cystes) chez les femelles d'Artémies adultes a été disséqué et conservé à −70°C. Ces tissus ont été utilisés pour les extractions de protéines et la solution a servi au dosage des protéines par la méthode de Bradford. Les protéines ont ensuite été analysées par électrophorèse bi-dimensionnelle et les taches obtenues sur les gels, comparées entre les deux espèces. À partir d'une approche comparative protéomique, les profils protéiques de l'ovaire et des utérus avec cystes, des deux espèces ont été analysés. Parmi les taches obtenues, 21 protéines avec des expressions différentes ont été reconnues. A la suite d'une spectrométrie de masse, trois de ces protéines, la 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) réductase, la protéine RING associée à BRCA1 (BARD1), et la tubuline β3, s'expriment différemment entre les deux espèces. De même quelques autres protéines, avec des fonctions inconnues, ont montré une sur-expression dans les gonades de A. parthenogenetica.


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Affiliations: 1: Department of Cell and Molecular Biology, School of Biology, University College of Science, University of Tehran, Tehran, Iran


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