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Antagonism of Sesame To the Root-Knot Nematode (Meloidogyne Incognita) On Okra in Tissue Culture

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For more content, see Nematology.

Excised roots and callus cultures of okra and sesame were raised on synthetic media. Sesame tissues cultured alone or with okra suppressed egg hatch, penetration of roots by juveniles, delayed adult development, and encouraged development of males in Meloidogyne incognita. Gall formation was inhibited on excised roots of okra by co-culturing with sesame. Sesame callus reduced penetration, discouraged nematode build-up in okra and caused an increase in numbers of males. Use of gnotobiotic technique for the establishment of antagonism of sesame to root-knot nematode on okra is described. Sucrose, vitamins, and iron chelate increased the numbers of nematodes penetrating roots and pH 7.0 was best for penetration.

Affiliations: 1: Punjab Agricultural University, Ludhiana-141004 (Punjab) India


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