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A Simple Method for the Immunocytochemical Demonstration of Cells Containing Antibody To Horseradish Peroxidase, Without Showing the Sites of Endogenous Peroxidase Activity

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image of Netherlands Journal of Zoology
For more content, see Archives Néerlandaises de Zoologie (Vol 1-17) and Animal Biology (Vol 53 and onwards).

Immunocytochemical methods for the demonstration of antibody to horseradish peroxidase (HPO) also visualize the endogenous peroxidase in tissue sections. Differentiation between the two cell populations involved is sometimes difficult. Fixation of the tissue sections with methanol appeared to abolish the activity of endogenous peroxidase, but it preserved the antigen binding capacity of the antibody to HPO, so that only the cells containing antibody were made visible in the immunocytochemical test. For the light microscopical study of immune processes the method with methanol fixation seems to be useful.

Affiliations: 1: Histological Laboratory, Free University, Amsterdam, The Netherlands


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