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Use of Esterase and Protein Patterns To Differentiate Two New Species of Aphelenchoides On Rice From Other Species of Aphelenchoides and From Ditylenchus Angustus and D. Myceliophagus

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Three populations of Aphelenchoides besseyi, one population of A. bicaudatus, A. arachidis, A. fragariae, A. hamatus, two undescribed species of Aphelenchoides from rice and Ditylenchus angustus and D. myceliophagus were analyzed by native and SDS-PAGE electrophoresis. The highly reproducible banding patterns of all species differed in number, concentration and relative mobility. Certain enzyme bands were common between the species whereas other bands were specific. There were no detectable differences in the protein and esterase banding patterns of nematodes extracted from cultures of Botrytis cinerea or Rhizoctonia cerealis. The stability and taxonomic usefulness of the α, β esterase banding patterns are discussed.

Affiliations: 1: Entomology & Nematology Department, AFRC, IACR, Rothamsted Experimental Station, Harpenden, Herts, AL5 2JQ, UK


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