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The Use of Heterologous Cloned Dna Probes To Distinguish Between Races of Meloidogyne Incognita

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For more content, see Nematology.

DNA from one population each of the four races of Meloidogyne incognita was restriction endonuclease digested and Southern blotted onto nitrocellulose filters. The filters were probed with a 7kb fragment consisting of the rDNA repeat of Caenorhabditis elegans (pCe7) and with a 3.lkb fragment, which contains part of the 18s ribosomal RNA gene from Schistosoma mansoni (pSM389). The results show that not only does rDNA from these species hybridise strongly with all four races of M. incognita, but the restriction fragment patterns produced allow the differentiation of two of the M. incognita races.

Affiliations: 1: ) Department of Biochemistry, Wellcome Centre for Parasitic Infections, Imperial College of Science, Technology and Medicine, London SW7 2AY; 2: ) Institute for Animal Health, Division of Immunology, Pirbright Laboratory Woking, GU24 ONF; 3: ) Rothamsted Experimental Station, Entomology & Nematology Department, Harpenden, Herts, AL5 2JQ; 4: ) Instituto de Salud Carlos , Centro Nacional de Microbiologia, Departamento de Parasitologia, 28220 Majadahonda (Madrid), Spain


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