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Cold Tolerance and Preconditioning of Infective Juveniles of Steinernema Kushidai (Nematoda: Steinernematidae)

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For more content, see Nematology.

Steinernema kushidai infective juveniles (IJs), that had been placed onto soil and stored at 5°C after recovery from artificial culture, did not survive well, with over 90% dying within 10 days. When the IJs were preconditioned at 10°C for over 8 days, a survival rate of over 50% was seen 100 days after storage at 5°C. This preconditioning at 10°C could also be carried out on IJs suspended in distilled water. Trehalose concentration in the IJs increased from almost undetectable levels to 1.4% of the dry weight when S. kushidai IJs in distilled water were preconditioned at 10°C for 20 days.

Affiliations: 1: Forestry and Forest Products Research Institute, P.O. Box 16, Tsukuba Norin Kenkyu Danchi, Ibaraki 305, Japan


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