Cookies Policy

This site uses cookies. By continuing to browse the site you are agreeing to our use of cookies.

I accept this policy

Find out more here

Characterization of a hyperpolarization-activated inward current in rat chemosensory petrosal neurons in vitro

No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
The full text of this article is not currently available.

Brill’s MyBook program is exclusively available on BrillOnline Books and Journals. Students and scholars affiliated with an institution that has purchased a Brill E-Book on the BrillOnline platform automatically have access to the MyBook option for the title(s) acquired by the Library. Brill MyBook is a print-on-demand paperback copy which is sold at a favorably uniform low price.

Access this article

+ Tax (if applicable)
Add to Favorites
You must be logged in to use this functionality

image of Primary Sensory Neuron
For more content, see Sensory Neuron.

Regulation of carotid body chemoafferent discharge in mammals plays an important role in the reflex control of ventilation. A non-selective blocker (cesium) of the inward rectifier is known to inhibit carotid body afferent discharge during hypoxia, but the underlying current in corresponding neurons of the petrosal ganglia has not been characterized. In this study we provide a detailed description of a voltage-dependent, inwardly rectifying, cation non-selective current, Ih, that was present in around 78% of cultured rat petrosal neurons. Activation of this current appeared to be the basis of the slowly developing depolarizing sag that was recorded under current clamp during application of hyperpolarizing current pulses. Under voltage clamp, Ih was activated at voltages negative to -60 mV and had an estimated reversal potential (Eh) of about -33.1±3.4 mV (n=20). Raising extracellular [K+]o caused a progressive increase in Ih and a positive shift in Eh, whereas reducing extracellular [Na+]o caused a small reduction in Ih and an opposite shift in Eh. Reducing extracellular [Cl-]o had no significant effect on Eh, though the amplitude of Ih decreased. Tail current analysis revealed that the activation curve for Ih was well fitted by the Boltzmann distribution, with V1/2=-90.6±2.2 mV (mean ± SEM; n=17) and slope factor k=10.8±0.5. Ih activated more rapidly at larger hyperpolarizations; elevated [K+]o or lowered [Na+]o increased the time constant (τ) of Ih activation. The time constant of deactivation of Ih at -60 mV was 317.1±31.9 ms (n=7). Extracellular cesium (10 mM) almost completely blocked Ih, whereas barium suppressed Ih by around 50%, at a similar concentration. These results, combined with the known sensitivity of the hypoxic afferent discharge to extracellular cesium, suggest that Ih likely plays an important physiological role during carotid body chemosensory signaling.


Full text loading...


Data & Media loading...

Article metrics loading...



Can't access your account?
  • Tools

  • Add to Favorites
  • Printable version
  • Email this page
  • Subscribe to ToC alert
  • Get permissions
  • Recommend to your library

    You must fill out fields marked with: *

    Librarian details
    Your details
    Why are you recommending this title?
    Select reason:
    Primary Sensory Neuron — Recommend this title to your library
  • Export citations
  • Key

  • Full access
  • Open Access
  • Partial/No accessInformation