{null=Molecular and morphological characterisations of two new species of Rotylenchus (Nematoda: Hoplolaimidae) from Iran, en=Molecular and morphological characterisations of two new species of <i>Rotylenchus</i> (Nematoda&#x3A; Hoplolaimidae) from Iran}

{null=Two new amphimictic species, Rotylenchus iranicus n. sp. and R. conicaudatus n. sp., are described. Rotylenchus iranicus n. sp. is characterised mainly by an offset hemispherical lip region with 5-6, rarely 7, annuli, stylet 39-44 μm long, vulva located at 53-65% and rounded tail with 4-9 annuli and typical smooth tip. Morphologically this species is related to R. montanus, R. provincialis and R. aqualamus. Rotylenchus conicaudatus n. sp. is characterised mainly by a slightly offset conoid-rounded lip region with four or five, rarely six annuli, stylet 27-32 μm long, vulva located at 52-63% and conoid-rounded tail with 10-16 annuli and a typical annulated tip. Morphologically this species is related to R. pumilus, R. abnormecaudatus, R. acuspicaudatus and R. provincialis. The results of the phylogenetic analysis based on sequences of the D2-D3 expansion regions of 28S and ITS1-rRNA genes confirmed the species differentiation. Phylogenetic relationships with other species were difficult to assign using D2-D3 expansion regions of 28S. However, using ITS1-rRNA, R. conicaudatus n. sp. presented a close relationship with R. unisexus, whilst R. iranicus n. sp. was closely related to R. conicaudatus n. sp., R. unisexus, R. incultus and R. laurentinus., en=<p>Two new amphimictic species, <i>Rotylenchus iranicus</i> n. sp. and <i>R. conicaudatus</i> n. sp., are described. <i>Rotylenchus iranicus</i> n. sp. is characterised mainly by an offset hemispherical lip region with 5-6, rarely 7, annuli, stylet 39-44 <i>&#x3BC;</i>m long, vulva located at 53-65% and rounded tail with 4-9 annuli and typical smooth tip. Morphologically this species is related to <i>R. montanus</i>, <i>R. provincialis</i> and <i>R. aqualamus</i>. <i>Rotylenchus conicaudatus</i> n. sp. is characterised mainly by a slightly offset conoid-rounded lip region with four or five, rarely six annuli, stylet 27-32 <i>&#x3BC;</i>m long, vulva located at 52-63% and conoid-rounded tail with 10-16 annuli and a typical annulated tip. Morphologically this species is related to <i>R. pumilus</i>, <i>R. abnormecaudatus</i>, <i>R. acuspicaudatus</i> and <i>R. provincialis</i>. The results of the phylogenetic analysis based on sequences of the D2-D3 expansion regions of 28S and ITS1-rRNA genes confirmed the species differentiation. Phylogenetic relationships with other species were difficult to assign using D2-D3 expansion regions of 28S. However, using ITS1-rRNA, <i>R. conicaudatus</i> n. sp. presented a close relationship with <i>R. unisexus</i>, whilst <i>R. iranicus</i> n. sp. was closely related to <i>R. conicaudatus</i> n. sp., <i>R. unisexus</i>, <i>R. incultus</i> and <i>R. laurentinus</i>.</p>}

Affiliations: 1: Department of Plant Protection, Faculty of Agricultural Sciences and Engineering, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran; 2: Plant Pathology Department, College of Agriculture, Tarbiat Modares University, Tehran, Iran;, Email: Pourjame@modares.ac.ir; 3: Plant Pathology Department, College of Agriculture, Tarbiat Modares University, Tehran, Iran; 4: Institute for Sustainable Agriculture (IAS), Spanish National Research Council (CSIC), Alameda del Obispo s/n, Apdo. 4084, 14080 Córdoba, Spain