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Determination of cellular DNA content of Iberian salamanders by flow cytometry

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The DNA content per diploid nucleus (2 C-value) was determined for the eight species of Iberian salamanders by flow cytometry. All species showed high values, which are characteristic of Caudata. The species with the lowest value was Pleurodeles waltl (48.25 pg DNA/cell) and Salamandra salamandra had the highest values (70.55 pg DNA/cell). There were no statistical differences between sexes in any of the eight species and DNA content was not directly related with phylogeny. The values obtained are often higher than previously published estimates obtained with older and potentially less accurate methodologies. We discuss the possible relationship between cellular DNA content and reproductive biology.

Se determinó el contenido en ADN por núcleo diploide (valor 2-C) por citometría de flujo de ocho especies de salamandras ibéricas. Todas las especies mostraron altos valores, lo que es característico de Caudata. La especie con el menor valor fue Pleurodeles waltl (48,25 pg ADN/célula) mientras Salamandra salamandra tuvo las valores más altos (70,55 pg ADN/célula). No se encontraron diferencias estadísticas entre sexos en ninguna de las 8 especies y el contenido en ADN no se relacionó directamente con la filogenia. Los valores obtenidos son, a menudo, más altos que los publicados previamente con técnicas más antigüas, y potencialmente menos precisas. Discutimos las posibles relaciones entre el contenido en ADN celular y la biología reproductiva de cada especie.

Affiliations: 1: Departamento de Biología Animal, Universidad de Salamanca, 37071 Salamanca, Spain; 2: Departamento de Biología Animal, Universidad de Salamanca, 37071 Salamanca, Spain; Centro de Biología Ambiental, Faculdade de Cíências, Universidade de Lisboa, Campo Grande, Lísboa, P-1700, Portugal; 3: Museo Nacional de Ciencias Naturales, José Gutierrez Abascal; 4: Servicio de Citometría, Universidad de Salamanca, Spain


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