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Full Access Isolation and characterization of six polymorphic microsatellite loci for the Malagasy spider tortoise, Pyxis arachnoides and cross-amplification in Pyxis planicauda

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Isolation and characterization of six polymorphic microsatellite loci for the Malagasy spider tortoise, Pyxis arachnoides and cross-amplification in Pyxis planicauda

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Pyxis arachnoides is a Critically Endangered Malagasy tortoise for which little data about its natural history and genetic variation are available. This lack of knowledge complicates undertaking conservation measures considering Pyxis genetic diversity. Here, we describe six new microsatellites for Pyxis arachnoides from wild specimens and several localities, and the cross amplification of two Astrochelys radiata microsatellites. Of these microsatellites, two are monomorphic among the three distinct subspecies and a closely related species, while the remaining six are polymorphic. Microsatellite data are needed for this species to study the inter- and intra-subspecies genetic diversity, especially for the highly endangered subspecies P. a. brygooi, which currently occurs only in remote localities. The availability of polymorphic microsatellites will allow identifying the population of origin of individuals and will aid in justifying their management as separate evolutionary lineages for conservation purposes. The rationale is to preserve both evolutionary processes and adaptive potential.

Affiliations: 1: 1School of Biosciences, Cardiff University, Biomedical Science Building, Museum Avenue, Cardiff CF10 3AX, Wales, UK; 2: 2CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Campus Agrário de Vairão, 4485-661 Vairão, Portugal; 3: 4CIRAD, UPR AGIRs, Antananarivo 101, Madagascar and CIRAD, UPR AGIRs, 34398 Montpellier, France; 4: 5Omaha’s Henry Doorly Zoo and Aquarium’s Center for Conservation and Research, Omaha, Nebraska, USA

Pyxis arachnoides is a Critically Endangered Malagasy tortoise for which little data about its natural history and genetic variation are available. This lack of knowledge complicates undertaking conservation measures considering Pyxis genetic diversity. Here, we describe six new microsatellites for Pyxis arachnoides from wild specimens and several localities, and the cross amplification of two Astrochelys radiata microsatellites. Of these microsatellites, two are monomorphic among the three distinct subspecies and a closely related species, while the remaining six are polymorphic. Microsatellite data are needed for this species to study the inter- and intra-subspecies genetic diversity, especially for the highly endangered subspecies P. a. brygooi, which currently occurs only in remote localities. The availability of polymorphic microsatellites will allow identifying the population of origin of individuals and will aid in justifying their management as separate evolutionary lineages for conservation purposes. The rationale is to preserve both evolutionary processes and adaptive potential.

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/content/journals/10.1163/15685381-00002866
2013-01-01
2016-12-02

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