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Resistance of morphological and behavioral sexual traits of the palmate newt (Lissotriton helveticus) to bacterial lipopolysaccharide treatment

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Infectious diseases are considered as a significant factor in the global decline of amphibians. In some vertebrates, the assessment of the individual sexual traits can be useful for assessment of their health status and immunocompetence due to trade-off between them and investment in the immune system. Our aim here was to determine whether the trade-off between the expression of sexual morphological and behavioral traits and investment in the immune system is present in an urodele, the Palmate newt (Lissotriton helveticus). The groups of males were injected by solutions of proinflammatory agent, lipopolysaccharide (LPS) from Escherichia coli serotype O:55:B5, at dosages toxic to vertebrates (2 and 10 mg/kg of body mass) or by saline solution only (control groups). They were subsequently measured for variations in body condition and expression of both morphological (filament length, hind-foot-web, crest) and behavioral (courtship frequency) sexual traits. The injection of either LPS or saline solution did not cause any adverse effect on health in any male of all groups. No significant differences in any of the sexual traits were observed between two groups of males injected by LPS and control groups of males indicating the absence of a trade-off between immune response and expression of sexual traits. Our result suggests that measuring morphological or behavioral sexual traits may not be a useful method for monitoring emergence of infectious diseases in the palmate newt.

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1. Ahtiainen J.J. , Alatalo R.V. , Mappes J. , Vertainen L. ( 2004): "Deacreased sexual signaling reveals reduced viability in small populations of the drumming wolf spider Hygrolycosa rubrofasciata ". Proc. R. Soc. Lond. B Vol 271: 1839- 1845. http://dx.doi.org/10.1098/rspb.2004.2791
2. Băncilă R.I. , Hartel T. , Plaiasu R. , Smets J. , Cogalniceanu D. ( 2010): "Comparing three body condition indices in amphibians: a case study of yellow-bellied toad Bombina variegate ". Amphib. Reptil. Vol 31: 558- 562. http://dx.doi.org/10.1163/017353710X518405
3. Berczi I. , Bertók L. , Bereznai T. ( 1966): "Comparative studies on the toxicity of Escherichia colilipopolysaccharide endotoxin in various animal species". Can. J. Microbiol. Vol 12: 1070- 1071. http://dx.doi.org/10.1139/m66-143
4. Bicego K.C. , Branco L.G.S. ( 2002): "Discrete electrolytic lesion of the preoptic area prevents LPS-induced behavioral fever in toads". J. Exp. Biol. Vol 205: 3513- 3518.
5. Bicego K.C. , Steiner A.A. , Antunes-Rodrigues J. , Branco L.G.S. ( 2002): "Indomethacin impairs LPS-induced behavioral fever in toads". J. Appl. Physiol. Vol 93: 512- 516. http://dx.doi.org/10.1152/japplphysiol.00121.2002
6. Birkhead T.R. , Fletcher F. , Pellatt E.J. ( 1998): "Sexual selection in the zebra finch Taeniopygia guttata: condition, sex traits and immune capacity". Behav. Ecol. Sociobiol. Vol 44: 179- 191. http://dx.doi.org/10.1007/s002650050530
7. Bleicker P.A. , Rollins-Smith L.A. , Jacobs D.M. , Cohen N. ( 1983): "Mitogenic responses of frog lymphocytes to crude and purified preparations of bacterial lipopolysaccharide (LPS)". Dev. Comp. Immunol. Vol 7: 483- 496. http://dx.doi.org/10.1016/0145-305X(83)90033-2
8. Brown G.P. , Shilton C.M. , Shine R. ( 2011): "Measuring amphibian immunocompetence: validation of the phytohemagglutinin skin-swelling assay in the cane toad, Rhinella marina ". Methods Ecol. Evol. Vol 2: 341- 348. http://dx.doi.org/10.1111/j.2041-210X.2011.00090.x
9. Bugbee T.M. , Ruben L.N. , Beard M.E. , Zettergren L.D. ( 1983): "Antibody-production by different sites and cyclophosphamide-induced immunosuppression of the TNP-LPS response in the Grass frog Rana pipiens ". Dev. Comp. Immunol. Vol 7: 569- 574. http://dx.doi.org/10.1016/0145-305X(83)90042-3
10. Chen G. , Robert J. ( 2011): "Antiviral immunity in amphibians". Viruses Vol 3: 2065- 2086. http://dx.doi.org/10.3390/v3112065
11. Chilton P.M. , Embry C.A. , Mitchell T.C. ( 2012): "Effects of differences in lipid A structure on TLR4 pro-inflammatory signaling and inflammasome activation". Front. Immunol. Vol 3: 154. http://dx.doi.org/10.3389/fimmu.2012.00154
12. Cornuau J.H. , Rat M. , Schmeller D.S. , Loyau A. ( 2012): "Multiple signals in the palmate newt: ornaments help when courting". Behav. Ecol. Sociobiol. Vol 66: 1045- 1055. http://dx.doi.org/10.1007/s00265-012-1355-y
13. Cunningham A.A. , Langton T.E.S. , Bennett P.M. , Lewin J.F. , Drury S.E.N. , Gough R.E. , MacGregor S.K. ( 1996): "Pathological and microbiological findings from incidents of unusual mortality of the common frog ( Rana temporaria)". Phil. Trans. R. Soc. Lond. B Vol 351: 1539- 1557. http://dx.doi.org/10.1098/rstb.1996.0140
14. Daszak P. , Cunningham A.A. , Hyatt A.D. ( 2010): "Emerging infectious diseases of wildlife – threats to biodiversity and human health". Science Vol 287: 443. http://dx.doi.org/10.1126/science.287.5452.443
15. Du Pasquier L. , Schwager J. , Flajnik M.F. ( 1989): "The immune system of Xenopus ". Ann. Rev. Immunol. Vol 7: 251- 275. http://dx.doi.org/10.1146/annurev.iy.07.040189.001343
16. Dunkelberger J.R. , Song W.-C. ( 2010): "Complement and its role in innate and adaptive immune responses". Cell Res. Vol 20: 34- 50. http://dx.doi.org/10.1038/cr.2009.139
17. Franco-Belussi L. , de Oliveira C. ( 2011): "Lipopolysaccharides induce changes in the visceral pigmentation of Eupemphix nattereri(Anura: Leiuperidae)". Zoology Vol 11: 298- 305. http://dx.doi.org/10.1016/j.zool.2011.05.001
18. Froese J.M.W. , Smits J.E.G. , Wickstrom M.L. ( 2005): "Evaluation of two methods for measuring nonspecific immunity in Tiger salamander ( Ambystoma trigrinum)". J. Wildlife Dis. Vol 41: 209- 217. http://dx.doi.org/10.7589/0090-3558-41.1.209
19. Green N. , Cohen N. ( 1977): "Effect of temperature on serum complement levels in the leopard frog Rana pipiens ". Dev. Comp. Immun. Vol 1: 59- 64. http://dx.doi.org/10.1016/S0145-305X(77)80051-7
20. Hayes T.B. , Case P. , Chui S. , Chung D. , Haeffele C. , Haston K. , Lee M. , Phoung Mai V. , Marjuoa Y. , Parker J. , Tsui M. ( 2006): "Pesticide mixtures, endocrine disruption, and amphibian declines: are we underestimating the impact?" Environ. Health Perspect. Vol 114( Suppl. 1): 40- 50. http://dx.doi.org/10.1289/ehp.8051
21. Hegyi G. , Møller A.P. , Eens M. , Garamszegi L.L. ( 2009): "Prevalence of avian influenza and sexual selection in ducks". Behav. Ecol. Vol 20: 1289- 1294. http://dx.doi.org/10.1093/beheco/arp133
22. Horton T.L. , Ritchie P. , Watson M.D. , Horton J.D. ( 1996): "NK-like activity against allogeneic tumour cells demonstrated in the spleen of control and thymectomized Xenopus ". Immunol. Cell Biol. Vol 74: 365- 373. http://dx.doi.org/10.1038/icb.1996.64
23. Jacot A. , Scheuber H. , Kurtz J. , Brinkhof M.W.G. ( 2005): "Juvenile immune status affects the expression of a sexually selected trait in field crickets". J. Evol. Biol. Vol 18: 1060- 1068. http://dx.doi.org/10.1111/j.1420-9101.2005.00899.x
24. Karima R. , Matsumoto S. , Higashi H. , Matsushima K. ( 1999): "The molecular pathogenesis of endotoxic shock and organ failure". Mol. Med. Today Vol 5: 123- 132. http://dx.doi.org/10.1016/S1357-4310(98)01430-0
25. Kaufman J. , Volk H. ( 1994): "The salamander immune system: right and wrong". Axolotl Newsl. Vol 23: 7- 23.
26. Lee K.A. , Martin L.B. , Wikelski M.C. ( 2005): "Responding to inflammatory challenges is less costly for a successful avian invader, the house sparrow ( Passer domesticus), than its less-invasive congener". Oecologia Vol 145: 244- 251. http://dx.doi.org/10.1007/s00442-005-0113-5
27. Leman J.C. , Weddle C.B. , Gershman S.N. , Kerr A.M. , Ower G.D. , St John J.M. , Vogel L.A. , Sakaluk S.K. ( 2009): "Lovesick: immunological costs of mating to male sagebrush crickets". J. Evol. Biol. Vol 22: 163- 171. http://dx.doi.org/10.1111/j.1420-9101.2008.01636.x
28. Llewellyn D. , Brown G.P. , Thompson M.B. , Shine R. ( 2011): "Behavioral responses to immune-system activation in an Anuran (the Cane toad, Bufo marinus): field and laboratory studies". Physiol. Biochem. Zool. Vol 84: 77- 86. http://dx.doi.org/10.1086/657609
29. Lopes P.C. , Adelman J. , Wingfield J.C. , Bentley G.E. ( 2012): "Social context modulates sickness behavior". Behav. Ecol. Sociobiol. Vol 66: 1421- 1428. http://dx.doi.org/10.1007/s00265-012-1397-1
30. Mangoni M.L. , Epand R.F. , Rosenfeld Y. , Peleg A. , Barra D. , Epand R.M. , Shai Y. ( 2008): "Lipopolysaccharide, a key molecule involved in the synergism between temporins in inhibiting bacterial growth and in endotoxin neutralization". J. Biol. Chem. Vol 283: 22907- 22917. http://dx.doi.org/10.1074/jbc.M800495200
31. Manning M.J. , Horton J.D. ( 1982): "RES structure and function of the amphibia". In: The Reticuloendothelial System, Vol Vol. 3. Phylogeny and Ontogeny, p.  424- 459. Cohen N. , Sigel M.M. , Eds, Plenum, New York.
32. Maule A.G. , VanderKooi S.P. ( 1999): "Stress-induced immune-endocrine interaction". In: Stress Physiology in Animals, p.  205- 245. Balm P.H.M. , Ed., Sheffield Academic Press, Sheffield, UK.
33. Moore K.S. , Bevins C.L. , Brasseur M.M. , Tomassini N. , Turner K. , Eck H. , Zasloff M. ( 1991): "Antimicrobial peptides in the stomach of Xenopus laevis ". J. Biol. Chem. Vol 266: 19851- 19857.
34. Owen-Ashley N.T. , Wingfield J.C. ( 2006): "Seasonal modulation of sickness behavior in free-living northwestern song sparrows ( Melospiza melodia morphna)". J. Exp. Biol. Vol 209: 3062- 3070. http://dx.doi.org/10.1242/jeb.02371
35. Piccioni M. , Monari C. , Kenno S. , Pericolini E. , Gabrielli E. , Pietrella D. , Perito S. , Bistoni F. , Kozel T.R. , Vecchiarelli A. ( 2013): "A purified capsular polysaccharide markedly inhibits inflammatory response during endotoxic shock". Infect. Immun. Vol 81: 90- 98. http://dx.doi.org/10.1128/IAI.00553-12
36. Raffel T.R. , LeGros R.P. , Love B.C. , Rohr J.R. , Hudson P.J. ( 2009): "Parasite age-intensity relationships in red-spotted newts: Does immune memory influence salamander disease dynamics?" Intern. J. Parasitol. Vol 39: 231- 241. http://dx.doi.org/10.1016/j.ijpara.2008.06.011
37. Reilly D.D. , Tomassini N. , Bevins C.L. , Zasloff M. ( 1994): "A paneth cell analogue in Xenopussmall intestine expresses antimicrobial peptide genes: conservation of an intestinal host-defense system". J. Histochem. Cytochem. Vol 42: 697- 704. http://dx.doi.org/10.1177/42.6.8189032
38. Reinert L.K. , Harper L.K. , Woodhams D.C. , Rollins-Smith L.A. ( 2010): "Immune defenses against Batrachochytrium dendrobatidis, a fungus linked to global amphibian declines, in the South African clawed frog, Xenopus laevis ". Infect. Immun. Vol 78: 3981- 3992. http://dx.doi.org/10.1128/IAI.00402-10
39. Rollins-Smith L.A. , Woodhams D.C. ( 2012): "Amphibian immunity – staying in tune with the environment". In: Ecoimmunology, p.  92- 143. Demas G.E. , Nelson R.J. , Eds, Oxford University Press, New York.
40. Rollins-Smith L.A. , Ramsey J.P. , Reinert L.K. , Woodhams D.C. , Livo L.J. , Carey C. ( 2009): "Immune defenses of Xenopus laevisagainst Batrachochytrium dendrobatidis ". Front. Biosci. Vol 1: 68- 91. http://dx.doi.org/10.2741/s8
41. Salvadori F. , Tournefier A. ( 1996): "Activation by mitogens and superantigens of axolotl lymphocytes: functional characterization and ontogenic study". Immunology Vol 88: 586- 592. http://dx.doi.org/10.1046/j.1365-2567.1996.d01-685.x
42. Schadich E. ( 2009): "Skin peptide activities against opportunistic bacterial pathogens of the African Clawed Frogs ( Xenopus laevis) and three Litoriafrogs". J. Herpetol. Vol 43: 173- 183. http://dx.doi.org/10.1670/08-113R1.1
43. Schadich E. , Cole A.L.J. ( 2010): "Pathogenicity of opportunistic bacterial pathogens Aeromonas hydrophila, Klebsiella pneumoniaeand Proteus mirabilistowards New Zealand Litoria ewingiifrogs". Comp. Med. Vol 60: 114- 117.
44. Schadich E. , Cole A.L.J. , Squire M. , Mason D. ( 2009): "Effect of the pesticide carbaryl on the production of the skin antimicrobial peptides of Litoria raniformisfrogs". Aust. J. Ecotox. Vol 15: 17- 24.
45. Schadich E. , Mason D. , Cole A.L. ( 2013): "Neutralization of bacterial endotoxins by frog antimicrobial peptides". Microbiol. Immunol. Vol 57: 159- 161. http://dx.doi.org/10.1111/1348-0421.12012
46. Sherman E. , Baldwin L. , Fernandez G. , Deurell E. ( 1991): "Fever and thermal tolerance in the toad Bufo marinus ". J. Therm. Biol. Vol 16: 297- 301. http://dx.doi.org/10.1016/0306-4565(91)90021-S
47. Sherman E. , Stephens A. ( 1998): "Fever and metabolic rate in the toad Bufo marinus ". J. Therm. Biol. Vol 23: 49- 52. http://dx.doi.org/10.1016/S0306-4565(97)00045-4
48. Todd B.D. ( 2007): "Parasites Lost? An overlooked hypothesis for the evolution of alternative reproductive strategies in amphibians". Am. Nat. Vol 170: 793- 799. http://dx.doi.org/10.1086/521958
49. Woodhams D.C. , Rollins-Smith L.A. , Briggs C.J. , Vredenburg V.T. , Simon M.A. , Billheimer D. , Shakhtour B. , Shyr Y. , Harris R.N. ( 2007): "Symbiotic bacteria contribute to innate immune defenses of the threatened mountain yellow-legged frog, Rana muscosa ". Biol. Cons. Vol 138: 390- 398. http://dx.doi.org/10.1016/j.biocon.2007.05.004
50. Zimmerman L.M. , Vogel L.A. , Bowden R.M. ( 2010): "Understanding the vertebrate immune system: insights from the reptilian perspective". J. Exp. Biol. Vol 213: 661- 671. http://dx.doi.org/10.1242/jeb.038315
51. Zou J. , Bird S. , Minter R. , Horton J. , Cunningham C. , Secombes C.J. ( 2000): "Molecular cloning of the gene for interleukin-1β from Xenopus laevisand analysis of expression in vivo and in vitro". Immunogenetics Vol 51: 332- 338. http://dx.doi.org/10.1007/s002510050627
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2014-01-01
2017-10-20

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