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Isolation and characterization of microsatellites markers for two South American frogs (Leptodactylus bufonius and L. chaquensis) using next generation sequencing

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Leptodactylus bufonius (Vizcacheras’ white-lipped frog) and L. chaquensis (Cei’s white-lipped frog) are pond-breeding frogs that inhabit the Chaco and surrounding savanna-like formations in South America. Throughout the Chacoan plain, the combined impacts of livestock and forestry practices have led to a highly fragmented landscape and an impoverished ecological system, threatening local species. We cloned and characterized new microsatellite markers for both species. These markers will be useful for behavioral and conservation genetic studies of populations throughout this threatened habitat.

Affiliations: 1: 1Programa de Pós-Graduação em Ecologia e Conservação, Universidade Federal de Mato Grosso do Sul, Cidade Universitária, 79070-900 Campo Grande, MS, Brazil; 2: 2Department of Ecology and Evolutionary Biology, Cornell University, Ithaca, NY 14853-2701, USA; 3: 3Departamento de Morfologia e Fisiologia Animal, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, “Júlio de Mesquita Filho”, 14884-900 Jaboticabal, SP, Brazil


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