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Genetic structure and differentiation of the fire salamander Salamandra salamandraat the northern margin of its range in the Carpathians

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Amphibian populations occurring at the margin of the species range exhibit lower genetic variation due to strong genetic drift and long-term isolation. Limited mobility and site fidelity together with habitat changes may accelerate genetic processes leading to local extinction. Here, we analyze genetic variation of the fire salamander subspecies Salamandra s. salamandrainhabiting the Outer Carpathian region in Poland, at the northern border of its distribution. Nuclear DNA polymorphism based on 10 microsatellite loci of 380 individuals sampled in 11 populations were analysed to measure gene flow between subpopulations and possible long-term isolation. Mitochondrial DNA control region analysis among 17 individuals representing 13 localities was used to detect the origin of populations which colonized Northern Europe after the last glaciation. Overall, pairwise F ST’s and AMOVA test of ‘among group’ variation showed little differences in the allele frequencies and relatively high local gene flow. However, Bayesian clustering results revealed subtle structuring between eastern and western part of the studied region. Two extreme marginal populations from the Carpathian Piedmont revealed reduced genetic variation which may be attributed to strong influence of genetic drift. Only one mitochondrial DNA haplotype (type IIb) was found in all individuals and suggest that after the Last Glacial Maximum Salamandra salamandramigrated to the North-Western Europe from the single glacial refugium placed in the Balkan Peninsula.

Affiliations: 1: 1Department of Evolutionary Biology and Conservation of Vertebrates, University of Wrocław, Sienkiewicza 21, 50-355 Wrocław, Poland; 2: 2Institute of Environmental Sciences, Jagiellonian University, Gronostajowa 7, 30-387 Kraków, Poland; 3: 3Faculty of Biological Sciences, University of Zielona Góra, Prof. Z. Szafrana 15, 65-516 Zielona Góra, Poland

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1. Allendorf F.W. , Luikart G. , Aitken S.N. ( 2013): Conservation and the Genetics of Populations. Wiley-Blackwell, UK.
2. Andersen L.W. , Fog K. , Damgaard C. ( 2004): "Habitat fragmentation causes bottlenecks and inbreeding in the European tree frog ( Hyla arborea)". Proc. R. Soc. Lond. B Vol 271: 1293- 1302. http://dx.doi.org/10.1098/rspb.2004.2720
3. Arif I.A. , Khan H.A. , Bakhali A.H. , Homaidan A.A.A. , Farhan A.H.A. , Sadoon M.A. , Shobrak M. ( 2011): "DNA marker technology for wildlife conservation". Saudi J. Biol. Sci. Vol 18: 219- 225. http://dx.doi.org/10.1016/j.sjbs.2011.03.002
4. Bar-David S. , Segev O. , Peleg N. , Hill N. , Templeton A.R. , Schultz C.B. , Blaustein L. ( 2007): "Long-distance movements by fire salamanders ( Salamandra infraimmaculata) and implications for habitat fragmentation". Isr. J. Ecol. Evol. Vol 53: 143- 159. http://dx.doi.org/10.1080/15659801.2007.10639579
5. Beebee T.J.C. , Rowe G. ( 1999): "Microsatellite analysis of natterjack toad Bufo calamitaLaurenti populations: consequences of dispersal from a Pleistocene refugium". Biol. J. Linn. Soc. Vol 69: 367- 381. http://dx.doi.org/10.1111/j.1095-8312.2000.tb01211.x
6. Beebee T.J.C. ( 2005): "Conservation genetics of amphibians". Heredity Vol 95: 423- 427. http://dx.doi.org/10.1038/sj.hdy.6800736
7. Blank L. , Sinai I. , Bar-David S. , Peleg N. , Segev O. , Sadeh A. , Kopelman N.M. , Templeton A.R. , Merilä J. , Blaustein L. ( 2012): "Genetic population structure of the endangered fire salamander ( Salamandra infraimmaculata) at the southernmost extreme of its distribution". Anim. Conserv. Vol 16: 412- 421. http://dx.doi.org/10.1111/acv.12009
8. Chapuis M.P. , Estoup A. ( 2007): "Microsatellite null alleles and estimation of population differentiation". Mol. Biol. Evol. Vol 24: 621- 631. http://dx.doi.org/10.1093/molbev/msl191
9. Cornuet J.M. , Luikart G. ( 1997): "Description and power analysis of two tests for detecting recent population bottlenecks from allele frequency data". Genetics Vol 144: 2001- 2014.
10. Dempster A.P. , Laird N.M. , Rubin D.B. ( 1977): "Maximum likelihood from incomplete data via the EM algorithm". J. R. Stat. Soc. B Vol 39: 1- 38.
11. Di Rienzo A. , Peterson A. , Garza J.C. , Valdes A.M. , Slatkin M. , Freimer N.B. ( 1994): "Mutational processes of simple-sequence repeat loci in human populations". Proc. Natl. Acad. Sci. USA Vol 91: 3166- 3170. http://dx.doi.org/10.1073/pnas.91.8.3166
12. Dyer R.J. , Nason J.D. ( 2004): "Population graphs: the graph theoretic shape of genetic structure". Mol. Ecol. Vol 13: 1713- 1727. http://dx.doi.org/10.1111/j.1365-294X.2004.02177.x
13. Earl D.A. , von Holdt B.M. ( 2012): "STRUCTURE HARVESTER: a website and program for visualizing STRUCTURE output and implementing the Evanno method". Conserv. Genet. Resour. Vol 4: 359- 361. http://dx.doi.org/10.1007/s12686-011-9548-7
14. Eckert C.G. , Samis K.E. , Lougheed S.C. ( 2008): "Genetic variation across species’ geographical ranges: the central-marginal hypothesis and beyond". Mol. Ecol. Vol 17: 1170- 1188. http://dx.doi.org/10.1111/j.1365-294X.2007.03659.x
15. Evanno G. , Regnaut S. , Goudet J. ( 2005): "Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study". Mol. Ecol. Vol 14: 2611- 2620. http://dx.doi.org/10.1111/j.1365-294X.2005.02553.x
16. Excoffier L. , Foll M. , Petit R.J. ( 2009): "Genetic consequences of range expansions". Annu. Rev. Ecol. Evol. Syst. Vol 40: 481- 501. http://dx.doi.org/10.1146/annurev.ecolsys.39.110707.173414
17. Excoffier L. , Lischer H.L. ( 2010): "Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows". Mol. Ecol. Res. Vol 10: 564- 567. http://dx.doi.org/10.1111/j.1755-0998.2010.02847.x
18. Frankham R. ( 2005): "Genetics and extinction". Biol. Conserv. Vol 126: 131- 140. http://dx.doi.org/10.1016/j.biocon.2005.05.002
19. Frankham R. ( 2010): "Challenges and opportunities of genetic approaches to biological conservation". Biol. Conserv. Vol 143: 1919- 1927. http://dx.doi.org/10.1016/j.biocon.2010.05.011
20. García-Ramos G. , Kirkpatrick M. ( 1997): "Genetic models of adaptation and gene flow in peripheral populations". Evolution Vol 51: 21- 28. http://dx.doi.org/10.2307/2410956
21. Garner T.W.J. , Pearman P.B. , Angelone S. ( 2004): "Genetic diversity across a vertebrate species’ range: a test of the centralperipheral hypopthesis". Mol. Ecol. Vol 13: 1047- 1053. http://dx.doi.org/10.1111/j.1365-294X.2004.02119.x
22. Głowaciński Z. , Rafiński J. , Eds ( 2003): Atlas of the Amphibians and Reptiles of Poland. Status-Distribution-Conservation. Biblioteka Monitoringu Środowiska, Warszawa-Kraków.
23. Goudet J. ( 2011): FSTAT, a Program to Estimate and Test Gene Diversities and Fixation Indices, Version 2.9.3. http://www.unil.ch/izea/softwares/fstat.html.
24. Hall T.A. ( 1999): "BIOEDIT: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT". Nucleic Acids Symp. Ser. Vol 41: 95- 98.
25. Hampe A. , Petit R.J. ( 2005): "Conserving biodiversity under climate change: the rear edge matters". Ecol. Lett. Vol 8: 461- 467. http://dx.doi.org/10.1111/j.1461-0248.2005.00739.x
26. Hartel T. , Schweiger O. , Öllerer K. , Cogălniceanu D. , Arntzen J.W. ( 2010): "Amphibian distribution in a traditionally managed rural landscape of eastern Europe: probing the effect of landscape composition". Biol. Conserv. Vol 143: 1118- 1124. http://dx.doi.org/10.1016/j.biocon.2010.02.006
27. Hewitt G.M. ( 1999): "Post-glacial recolonization of European biota". Biol. J. Linn. Soc. Vol 68: 87- 112. http://dx.doi.org/10.1111/j.1095-8312.1999.tb01160.x
28. Jehle R. , Arntzen J.W. ( 2002): "Microsatellite markers in amphibian conservation genetics". J. Herpetol. Vol 12: 1- 9.
29. Kopelman N.M. , Mayzel J. , Jakobsson M. , Rosenberg N.A. , Mayrose I. ( 2015): "CLUMPAK: a program for identifying clustering modes and packaging population structure inferences across K". Mol. Ecol. Res.DOI: .
30. Kuzmin S. , Papenfuss T. , Sparreboom M. , Ugurtas I.H. , Anderson S. , Beebee T. , Denoël M. , Andreone F. , Anthony B. , Schmidt B. , Ogrodowczyk A. , Ogielska M. , Bosch J. , Tarkhnishvili D. , Ishchenko V. ( 2009): Salamandra salamandra. The IUCN Red List of Threatened Species. Version 2015.1. . Downloaded on 29 May 2015.
31. Manel S. , Schwartz M.K. , Luikart G. , Taberlet P. ( 2003): "Landscape genetics: combining landscape ecology and population genetics". Trends Ecol. Evol. Vol 18: 189- 197. http://dx.doi.org/10.1016/S0169-5347(03)00008-9
32. Najbar B. ( 1995): Płazy i gady Polski. Wyższa Szkoła Inżynierska w Zielonej Górze. Zielona Góra.
33. Ogrodowczyk A. , Ogielska M. , Kierzkowski P. , Maślak R. ( 2010): "Występowanie salamandry plamistej Salamandra s. salamandraLinnaeus 1758 na Dolnym Śląsku". Przyroda Sudetów Vol 13: 179- 192.
34. Ogrodowczyk A. ( 2011): Zasięg występowania, zmienność genetyczna i struktura wybranych populacji salamandry plamistej Salamandra s. salamandra(Linnaeus, 1758) na Dolnym Śląsku. Praca doktorska. Uniwersytet Wrocławski.
35. Pabijan M. , Babik W. , Rafiński J. ( 2005): "Conservation units in north-eastern populations of the Alpine newt ( Triturus alpestris)". Conserv. Genet. Vol 6: 307- 312. http://dx.doi.org/10.1007/s10592-004-7825-7
36. Pabijan M. , Babik W. ( 2006): "Genetic structure in northeastern populations of the Alpine newt ( Triturus alpestris): evidence for post-Pleistocene differentiation". Mol. Ecol. Vol 15: 2397- 2407. http://dx.doi.org/10.1111/j.1365-294X.2006.02954.x
37. Piry S. , Luikart G. , Cornuet J.M. ( 1999): "BOTTLENECK: a computer program for detecting recent reductions in the effective population size using allele frequency data". J. Hered. Vol 90: 502- 503. http://dx.doi.org/10.1093/jhered/90.4.502
38. Porras-Hurtado L. , Ruiz Y. , Santos C. , Phillips Ch. , Carracedo A. , Lareu M.V. ( 2013): "An overview of STRUCTURE: applications, parameter settings, and supporting software". Front. Genet. Vol 4: 1- 13. http://dx.doi.org/10.3389/fgene.2013.00098
39. Pritchard J.K. , Stephens M. , Donelly P. ( 2000): "Inference of population structure using multi-locus genotype data". Genetics Vol 155: 945- 959.
40. Prusak B. , Mitrus S. , Najbar B. , Pacholewska A. , Deas A. , Skonieczna K. , Górecki G. , Grzybowski G. , Hryniewicz A. , Wróblewski R. , Grzybowski T. ( 2013): "Population differentiation of the European pond turtle ( Emys orbicularis) in Poland inferred by the analysis of mitochondrial and microsatellite DNA: implications for conservation". Amphibia-Reptilia Vol 34: 451- 461. http://dx.doi.org/10.1163/15685381-00002898
41. Rice W.R. ( 1989): "Analyzing tables of statistical tests". Evolution Vol 43: 223- 225. http://dx.doi.org/10.2307/2409177
42. Rousset F. ( 2008): "Genepop’007: a complete reimplementation of the Genepop software for Windows and Linux". Mol. Ecol. Res. Vol 8: 103- 106. http://dx.doi.org/10.1111/j.1471-8286.2007.01931.x
43. Rousset F. ( 2014): Genepop 4.3 for Windows/Linux/Mac OS X. http://kimura.univ-montp2.fr/~rousset/Genepop.pdf.
44. Rowe G. , Beebee T.J.C. ( 2003): "Population on the verge of a mutational meltdown? Fitness costs genetic loads for an amphibian in the wild". Evolution Vol 57: 177- 181. http://dx.doi.org/10.1111/j.0014-3820.2003.tb00228.x
45. Schmidt B.R. , Schaub M. , Steinfartz S. ( 2007): "Apparent survival of the salamander Salamandra salamandrais low because of high migratory activity". Front. Zool. Vol 4: 19, DOI: . http://dx.doi.org/10.1186/1742-9994-4-19
46. Schmitt T. ( 2007): "Molecular biogeography of Europe: Pleistocene cycles and postglacial trends". Front. Zool. Vol 4: 11. DOI: .
47. Schulte U. , Küsters D. , Steinfartz S. ( 2007): "A PIT tag based analysis of annual movement patterns of adult fire salamanders ( Salamandra salamandra) in a middle European habitat". Amphibia-Reptilia Vol 28: 531- 536. http://dx.doi.org/10.1163/156853807782152543
48. Sexton J.P. , McIntyre P.J. , Angert A.L. , Rice K.J. ( 2009): "Evolution and ecology of species range limits". Annu. Rev. Ecol. Evol. Syst. Vol 40: 415- 436. http://dx.doi.org/10.1146/annurev.ecolsys.110308.120317
49. Slatkin M. ( 1987): "Gene flow and the geographic structure of natural populations". Science Vol 236: 787- 792. http://dx.doi.org/10.1126/science.3576198
50. Slatkin M. ( 1993): "Isolation by distance in equilibrium and nonequilibrium populations". Evolution Vol 47: 264- 279. http://dx.doi.org/10.2307/2410134
51. Sommer R.S. , Indqvist Ch.L. , Persson A. , Bringsøe H. , Rhodin A.J. , Schneeweiss N. , Širok P. , Bachmann L. , Fritz U. ( 2009): "Unexpected early extinction of the European pond turtle ( Emys orbicularis) in Sweden and climatic impact on its Holocene range". Mol. Ecol. Vol 18: 1252- 1262. http://dx.doi.org/10.1111/j.1365-294X.2009.04096.x
52. Sotiropoulos K. , Eleftherakos K. , Kalezić M.L. , Legakis A. , Polymeni R.M. ( 2008): "Genetic structure of the alpine newt, Mesotriton alpestris(Salamandridae, Caudata), in the southern limit of its distribution: implications for conservation". Biochem. System. Ecol. Vol 36: 297- 311. http://dx.doi.org/10.1016/j.bse.2007.10.002
53. Steinfartz S. , Veith M. , Tautz D. ( 2000): "Mitochondrial sequence analysis of Salamandrataxa suggests old splits of major lineages and postglacial recolonization of central Europe from distinct source populations of Salamandra salamandra ". Mol. Ecol. Vol 9: 397- 410. http://dx.doi.org/10.1046/j.1365-294x.2000.00870.x
54. Steinfartz S. , Küsters D. , Tautz D. ( 2004): "Isolation and characterization of polymorphic tetranucleotide microsatellite loci in the fire salamander Salamandra salamandra(Amphibia: Caudata)". Mol. Ecol. Notes Vol 4: 626- 628. http://dx.doi.org/10.1111/j.1471-8286.2004.00716.x
55. Steinfartz S. , Weitere M. , Tautz D. ( 2007): "Tracing the first step to speciation: ecological and genetic differentiation of a salamander population in a small forest". Mol. Ecol. Vol 16: 4550- 4561. http://dx.doi.org/10.1111/j.1365-294X.2007.03490.x
56. Tamura K. , Stecher G. , Peterson D. , Filipski A. , Kumar S. ( 2013): "MEGA6: molecular evolutionary genetics analysis version 6.0". Mol. Biol. Evol. Vol 30: 2725- 2729. http://dx.doi.org/10.1093/molbev/mst197
57. Thiesmeier B. ( 2004): Der Feuersalamander. Laurenti-Verlag, Bielefeld.
58. Van Oosterhout C. , Hutchinson W.F. , Willis D.P. , Shipley P. ( 2004): "MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data". Mol. Ecol. Notes Vol 4: 535- 538. http://dx.doi.org/10.1111/j.1471-8286.2004.00684.x
59. Weitere M. , Tautz D. , Neumann D. , Steinfartz S. ( 2004): "Adaptive divergence vs. environmental plasticity: trading local genetic adaptation of metamorphosis in salamanders". Mol. Ecol. Vol 13: 1665- 1677. http://dx.doi.org/10.1111/j.1365-294X.2004.02155.x
60. Zakrzewski M. ( 2007): Salamandra plamista. Rozmieszczenie, biologia i zagrożenia. Wydawnictwo Naukowe AP, Kraków.
61. Zieliński P. , Dudek K. , Stuglik M.T. , Liana M. , Babik W. ( 2014): "Single nucleotide polymorphisms reveal genetic structuring of the Carpathian newt and provide evidence of interspecific gene flow in the nuclear genome". PLoS ONE Vol 9( 5): e97431. DOI: .
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2015-09-07
2018-10-22

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