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The effect of photoperiod regimes on daily RNA:DNA ratio rhythms in Chinese soft-shelled turtles (Pelodiscus sinensis)

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The ratio of RNA to DNA is widely used to reflect instantaneous animal growth; however, little is known about its daily variation. Photoperiod can modify expression of internal clocks, providing animals with the flexibility to adapt to variable environments. This study focused on the influence of photoperiod regimes on the daily variation of RNA:DNA ratio in Pelodiscus sinensis. We randomly divided 260 turtles into four groups: constant dark (0L), 8 h light with 16 h dark (8L), 12 h light with 12 h dark (12L), and 16 h light with 8 h dark (16L). Turtles were housed under specific photoperiods for 15 days (fed for first 10 days then starved for 5 days), thereafter we sampled the tissues every 2 h for 24 h. We dissected forelimb muscles and measured the concentration of isolated RNA and DNA. There were rhythmic variations in the RNA:DNA ratio, even in turtles under continuous darkness, indicating that P. sinensis has circadian RNA:DNA ratio rhythms, and the rhythms were likely controlled by internal clocks. Additionally, the acrophase was advanced by two hours in constant darkness in contrast to the other three photoperiods, indicating that the photoperiod considerably modified the rhythm set by the internal clocks. Notably, the RNA:DNA ratio differed between photoperiod regimes, with 0L > 16L > 8L ≈ 12L, indicating the photoperiod may be a seasonal indicator for turtles to synchronize their physiological processes with environmental variations.

Affiliations: 1: College of Life Science, Hebei Normal University, Shijiazhuang, Hebei 050024, China

*Corresponding author; e-mail: zcyang@hebtu.edu.cn
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1. Akhtar M.S., Pal A.K., Sahu N.P., Ciji A., Meena D.K., Das P. (2013): "Physiological responses of dietary tryptophan fed Labeo rohita to temperature and salinity stress". J. Anim. Physiol. Anim. Nutr. Vol 97: 1075-1083. [Crossref]
2. Aschoff J. (1960): "Exogenous and endogenous components in circadian rhythms". Cold Spring Harbor Symp. Quant. Biol. Vol 25: 11-28. [Crossref]
3. Ayson F.G., Takemura A. (2006): "Daily expression patterns for mRNAs of GH, PRL, SL, IGF-I and IGF-II in juvenile rabbitfish, Siganus guttatus, during 24-h light and dark cycles". Gen. Comp. Endocrinol. Vol 149: 261-268. [Crossref]
4. Barrel G.K., Thrun L.A., Brown M.E., Viguie C., Karsch F.J. (2000): "Importance of photoperiodic signal quality to entrainment of the circannual reproductive rhythm of the ewe". Biol. Reprod. Vol 63: 769-774. [Crossref]
5. Bernreuther M., Herrmann J.P., Peck M.A., Temming A. (2012): "Growth energetics of juvenile herring, Clupea harengus L.: food conversion efficiency and temperature dependency of metabolic rate". J. Appl. Ichthyol. Vol 29: 331-340. [Crossref]
6. Bradshaw W.E., Holzapfel C.M. (2007): "Evolution of animal photoperiodism". Annu Rev Ecol Evol S Vol 38: 1-25. [Crossref]
7. Buckley L., Caldarone E., Ong T.L. (1999): "RNA-DNA ratio and other nucleic acid-based indicators for growth and condition of marine fishes". Hydrobiologia Vol 401: 265-277. [Crossref]
8. Chícharo L.M.Z., Chícharo M.A., Alves F., Amaral A., Pereira A., Regala J. (2001): "Diel variation of the RNA /DNA ratios in Crassostrea angulata and Ruditapes decussatus". J. Exp. Mar. Biol. Ecol. Vol 259: 121-129. [Crossref]
9. Chícharo M.A., Chícharo L., Valdez L., Lopez J.E., Re P. (1998): "Estimation of starvation and diel variation of RNA:DNA ratios in Sardina pilchardus larvae off north Spain". Mar. Ecol.: Prog. Ser. Vol 164: 273-283. [Crossref]
10. Ellis D.J., Firth B.T., Belan I. (2006): "Circadian ryhthm of behavioral thermoregulation in the sleepy lizard (Tiliqua rugosa)". Herpetologica Vol 62: 259-265. [Crossref]
11. Ellis D.J., Firth B.T., Belan I. (2008): "Interseasonal variation in the circadian rhythms of locomotor activity and temperature selection in sleepy lizards, Tiliqua rugosa". J. Comp. Physiol., A Vol 194: 701-712. [Crossref]
12. Esteves E., Pina T., Andrade J.P. (2009): "Diel and seasonal changes in nutritional condition of the anadromous twaite shad Alosa fallax fallax larvae". Ecol Freshw Fish. Vol 18: 132-144. [Crossref]
13. Falahatkar B., Poursaeid S., Efatpanah I., Meknatkhah B. (2012): "Effect of photoperiod manipulation on growth performance, physiological and hematological indices in juvenile Persian sturgeon, Acipenser persicus". J. World Aquacult. Soc. Vol 43: 679-687. [Crossref]
14. Fan M., Fox D.S., Cadena V. (2014): "Cyclic colour change in the bearded dragon Pogona vitticeps under different photoperiods". PLoS One Vol 9: e111504.
15. Farhat, Khan M.A. (2014): "Total sulfur amino acid requirement and cystine replacement value for fingerling stinging catfish, Heteropneustes fossilis". Aquaculture Vol 426-427: 270-281. [Crossref]
16. Firth B.T., Belan I., Kennaway D.J. (2006): "Persistence of a plasma melatonin rhythm in constant darkness and its inhibition by constant light in the sleepy lizard, Tiliqua rugosa". J. Pineal Res. Vol 41: 15-20. [Crossref]
17. Grimm C., Lehmann K., Clemmesen C., Brendelberger H. (2015): "RNA/DNA ratio is an early responding, accurate performance parameter in growth experiments of noble crayfish Astacus astacus (L.)". Aquacult. Res. Vol 46: 1937-1945. [Crossref]
18. Hou Y.Q., Wang L., Zhang W. (2012): "Protective effects of N-acetylcysteine on intestinal functions of piglets challenged with lipopolysaccharide". Amino Acids. Vol 43: 1233-1242. [Crossref]
19. Lee S.M.L., Wong W.P., Hiong K.C., Loong A.M., Chew S.F., Ip Y.K. (2006): "Nitrogen metabolism and excretion in the aquatic Chinese soft-shelled turtle, Pelodiscus sinensis, exposed to a progressive increase in ambient salinity". J. Exp. Zool. Vol 305A: 995-1009. [Crossref]
20. Menaker M., Wisner S. (1983): "Temperature-compensated circadian clock in the pineal of Anolis". Proc. Natl. Acad. Sci. U.S.A. Vol 80: 6119-6121. [Crossref]
21. Moore A.F., Kawasaki M., Menaker M. (2012): "Photic induction of locomotor activity is correlated with photic habitat in Anolis lizards". J. Comp. Physiol., A Vol 198: 193-201. [Crossref]
22. Oliveira C.C.V., Aparício R., Blanco-Vives B., Chereguini O., Martín I., Sánchez-Vazquez F.J. (2013): "Endocrine (plasma cortisol and glucose) and behavioral (locomotor and self-feeding activity) circadian rhythms in Senegalese sole (Solea senegalensis) exposed to light/dark cycles or constant light". Fish Physiol. Biochem. Vol 39: 479-487. [Crossref]
23. Olsson K., Nyström P., Stenroth P., Nilsson E., Svensson M., Granéli W. (2008): "The influence of food quality and availability on trophic position, carbon signature, and growth rate of an omnivorous crayfish". Can. J. Fish. Aquat. Sci. Vol 65: 2293-2304. [Crossref]
24. Peck M.A., Baumann H., Clemmesen C., Herrmann J.P., Moyano M., Temming A. (2015): "Calibrating and comparing somatic-, nucleic acid-, and otolith-based indicators of growth and condition in young juvenile European sprat (Sprattus sprattus)". J. Exp. Mar. Biol. Ecol. Vol 471: 217-225. [Crossref]
25. Pittendrigh C. (1960): "Circadian rhythms and the circadian organization of living systems". Cold Spring Harbor Symp. Quant. Biol. Vol 25: 159-184. [Crossref]
26. Reyes C., Milsom W.K. (2010): "Circadian and circannual rhythms in the metabolism and ventilation of red-eared sliders (Trachemys scripta elegans)". Physiol. Biochem. Zool. Vol 83: 283-298. [Crossref]
27. Rismiller P.D., Heldmaier G. (1982): "The effect of photoperiod on temperature selection in the European green lizard". Oecologia Vol 53: 222-226. [Crossref]
28. Rismiller P.D., Heldmaier G. (1988): "How photoperiod influences body temperature selection in the lizard Lacerta viridis". Oecologia Vol 75: 125-131. [Crossref]
29. Rismiller P.D., Heldmaier G. (1991): "Seasonal changes in daily metabolic patterns of Lacerta viridis". J. Comp. Physiol., B Vol 161: 482-488. [Crossref]
30. Rooker J.R., Holt G.J. (1996): "Application of RNA:DNA ratios to evaluate the condition and growth of larval and juvenile red drum (Sciaenops ocellatus)". Mar. Freshwater Res. Vol 47: 283-290. [Crossref]
31. Rosa R., Nunes M.L. (2003): "Seasonal changes in nucleic acids, amino acids and protein content in juvenile Norway lobster (Nephrops norvegicus)". Mar. Biol. Vol 143: 565-572. [Crossref]
32. Spellerberg I.F. (1974): "Influence of photoperiod and light intensity on lizards voluntary temperatures". Br. J. Herpetol. Vol 5: 412-420.
33. Stierhoff K.L., Targett T.E., Power J.H. (2009): "Hypoxia-induced growth limitation of juvenile fishes in an estuarine nursery: assessment of small-scale temporal dynamics using RNA:DNA". Can. J. Fish. Aquat. Sci. Vol 66: 1033-1047. [Crossref]
34. Tripathi M.K., Singh R., Pati A.K. (2015): "Daily and seasonal rhythms in immune responses of splenocytes in the freshwater snake, Natrix piscator". PLoS One Vol 10: e0116588.
35. Ultsch G.R., Wasser J.S. (1990): "Plasma ion balance of North American freshwater turtles during prolonged submergence in normoxic water". Comp. Biochem. Physiol. Vol 97A: 505-512. [Crossref]
36. Underwood H. (1992): "Hormones, brain, and behavior". In: Biology of the Reptilia, p.  229-297. Gans C., Crews D., Eds, Chicago.
37. Vieira S., Martins S., Hawkes L.A., Marco A., Teodósio M.A. (2014): "Biochemical indices and life traits of loggerhead turtles (Caretta caretta) from Cape Verde Islands". PLoS One Vol 9: e112181.
38. Wang Q.J., Hou Y.Q., Yi D. (2013): "Protective effects of N-acetylcysteine on alleviating acetic acid-induced colitis in a porcine model". BMC Gastroenterol. Vol 13: 133.
39. Xu Y.Q., Ding Z.K., Zhang H.Z., Liu L., Wang S.Q., Gorge J. (2009): "Different ratios of docosahexaenoic and eicosapentaenoic acids do not alter growth, nucleic acid and fatty acids of juvenile Cobia (Rachycentron canadum)". Lipids Vol 44: 1091-1104. [Crossref]
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/content/journals/10.1163/15685381-00003105
2017-05-30
2017-11-18

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