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Comparison of hematological parameters in two different high altitudinal populations of Batrachuperus pinchonii (Amphibian: Urodela)

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Hematological parameters are key to reflect the health status of animals and their physiological adaptation to the environment. However, few studies focused on the inter- and intra-specific variations of hematological parameters in hynobiid salamanders. Here, we examined the hematological parameters of the stream salamander, Batrachuperus pinchonii, originating from two different altitudinal populations to explore their intra-specific variation. Sexual dimorphism is only present in the erythrocyte count and males have higher mean values than females. The morphometric values of erythrocyte, hemoglobin concentration, and erythrocyte count of the high altitudinal (Jiajin) population were smaller than those of the lower altitudinal (Sandaoping) population; however, a significant difference between two populations was only revealed in the case of erythrocyte length via ANOVA. The results of linear regression showed that a significant relationship was present between body condition and erythrocyte length as well as the erythrocyte length to erythrocyte width ratio. Our findings suggest that the features of hematological parameters in B. pinchonii are reflected in the size of erythrocyte, and neither in erythrocyte count nor in hemoglobin concentration. These results provide a foundation for assessing and monitoring the health status of this salamander species, and furthermore, for understanding the physiological basis of altitudinal adaptation.

Affiliations: 1: 1Laboratory of Adaptation and Evolution of Aquatic Animals, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471023, Henan, China ; 2: 2Life Science Department, Luoyang Normal University, Luoyang 471022, Henan, China

*Corresponding author; e-mail:

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1. Allender M.C., Fry M.M. (2008): "Amphibian hematology". Vet. Clin. Exot. Anim. Vol 11: 463-480. [Crossref]
2. Andersson M. (1994): Sexual Selection. Princeton University Press, Princeton.
3. Arikan H., Çiçek K. (2014): "Haematology of amphibians and reptiles: a review". North-West J. Zool. Vol 10: 190-209.
4. Arikan H., Olgun K., Ilgaz Ç., Baran İ., Kumlutaş Y. (2003): "Erythrocyte size and number in Neurergus strauchii (Urodela: Salamandridae)". Russ. J. Herpetol. Vol 10: 163-166.
5. Atatür M.K., Arikan H., Mermer A. (1998): "Erythrocyte sizes of some urodeles from Turkey". Turk. J. Zool. Vol 22: 89-91.
6. Bǎncilǎ R.I., Hartel T., Plǎiaşu R., Smets J., Cogǎlniceanu D. (2010): "Comparing three body condition indices in amphibians: a case study of yellow-bellied toad Bombina variegata". Ampbibia-Reptilia Vol 31: 558-562. [Crossref]
7. Baraquet M., Grenat P.R., Salas N.E., Martino A.L. (2013): "Intraspecific variation in erythrocyte sizes among populations of Hynsiboas cordobae (Anura: Hylidae)". Acta Herpetol. Vol 8: 93-97.
8. Barriga-Vallejo C., Hernández-Gallegos O., Herbing I.H.V., López-Moreno A.E., Ruiz-Gómez M.L., Granados-Gonzalez G., Garduño-Paz M.V., Méndez-Sánchez J.F., Banda-Leak J., Davis A.K. (2015): "Assessing population health of the toluca axolotl Ambystoma rivulare (Taylor, 1940) from Mexico, using leukocyte profiles". Herpetol. Conserv. Bio. Vol 10: 592-601.
9. Biswas H.M., Boral M.C. (1986): "Changes of body fluid and hematology in toad and their rehabilitation following intermittent exposure to simulated high altitude". Int. J. Biometeorol. Vol 30: 189-197. [Crossref]
10. Cox R.M., Butler M.A., John-Alder H.B. (2007): "The evolution of sexual size dimorphism in reptiles". In: Sex, Size and Gender Roles: Evolutionary Studies of Sexual Size Dimorphism, p.  38-49. Fairbairn D.J., Blanchnhorn W.U., Székely T., Eds, Oxford University Press, Oxford. [Crossref]
11. Davis A.K. (2008): "Ontogenetic changes in erythrocyte morphology in larval mole salamanders, Ambystoma talpoideum, measured with image analysis". Comp. Clin. Pathol. Vol 17: 23-28. [Crossref]
12. Davis A.K. (2009): The Wildlife Leukocytes webpage: the ecologist’s source for information about leukocytes of wildlife species. Available at (accessed 4 May 2017).
13. Davis A.K., Cook K.C., Altizer S. (2004): "Leukocyte profiles of house finches with and without mycoplasmal conjunctivitis, a recently emerged bacterial disease". Ecohealth Vol 1: 362-373. [Crossref]
14. Davis A.K., Maerz J.C. (2008): "Sex-related differences in hematological stress indices of breeding paedomorphic mole salamanders". J. Herpetol. Vol 42: 197-201. [Crossref]
15. Davis A.K., Maney D.L., Maerz J.C. (2008): "The use of leukocyte profiles to measure stress in vertebrate: a review for ecologists". Funct. Ecol. Vol 22: 760-772. [Crossref]
16. Dessauer H.C. (1970): "Blood chemistry of reptiles: physiological and evolutionary aspects". In: Biology of Reptilia, Vol vol. 3, p.  1-72. Gans C., Parsons T.S., Eds, Academic Press, New York.
17. Ding X.Z., Liang C.N., Guo X., Wu X.Y., Wang H.B. (2014): "Physiological insight into the high-altitude adaptations in domesticated yaks (Bos grunniens) along the Qinghai-Tibetan Plateau altitudinal gradient". Livest. Sci. Vol 162: 233-239. [Crossref]
18. Fei L., Hu S.Q., Ye C.Y., Huang Y.Z. (2006): Fauna Sinica: Amphibia, Vol vol. 1. Science Press, Beijing.
19. Finkler M.S. (2013): "Sexual dimorphism in visceral organ mass and hematology in spotted salamanders, Ambystoma maculatum, before and after breeding". Copeia Vol 2013: 338-345. [Crossref]
20. Foxon G.E.H. (1964): "Blood and respiration". In: Physiology of the Amphibia, p.  151-209. Moore I.A., Ed., Academic Press, New York.
21. Friedmann G.B. (1971): "Altitudinal variation in the red blood cell count and haemoglobin content of urodele blood". Can. J. Zool. Vol 49: 565-568. [Crossref]
22. Frost D.R. (2017): Amphibian species of the world: an online reference. Version 6.0. Electronic database accessible at (accessed 31.5.2017). American Museum of Natural History, New York, USA.
23. Frýdlová P., Hnízdo J., Chylíková L., Šimková O., Cikánová V., Velenský P., Frynta D. (2013): "Morphological characteristics of blood cells in monitor lizards: is erythrocyte size linked to actual body size?" Integr. Zool. Vol S1: 39-45. [Crossref]
24. González-Morales J.C., Quintana E., Díaz-Albiter H., Guevara-Fiore P., Fajardo V. (2015): "Is erythrocyte size a strategy to avoid hypoxia in Wiegmann’s torquate lizards (Sceloporus torquatus)? Field evidence". Can. J. Zool. Vol 93: 377-382. [Crossref]
25. Gould S. (1975): "Allometry in primates, with emphasis on scaling and evolution of the brain". In: Approaches to Primate Paleobiology, p.  244-292. Szalay F.S., Ed., Karger, Basel, Switzerland.
26. Grenat P.R., Bionda C., Salas N.E., Martino A.L. (2010): "Variation in erythrocyte size between juveniles and adults of Odontophrynus americanus". Amphibia-Reptilia Vol 30: 141-145. [Crossref]
27. Guo K.J., Mi X.Q., Deng X.J. (2008): "Breeding ecology of Hynobius guabangshanensis". Chin. J. Ecol. Vol 27: 77-82.
28. Hansen M.J. (2005): "A method for correcting the relative weight (Wr) index for seasonal patterns in relative condition (Kn) with length as applied to walleye in Wisconsin". N. Am. J. Fish. Manage. Vol 25: 1256-1262. [Crossref]
29. Hasumi M. (1994): "Reproductive behavior of the salamander Hynobius nigrescens: monopoly of egg sacs during scramble competition". J. Herpetol. Vol 28: 264-267. [Crossref]
30. Hasumi M. (2015): "Social interactions during the aquatic breeding phase of the family Hynobiidae (Amphibia: Caudata)". Acta Ethol. Vol 18: 243-253. [Crossref]
31. He J.Z., Xiu M.G., Tang X.L., Yue F., Wang N.B., Yang S.B., Chen Q. (2013): "The different mechanisms of hypoxic acclimatization and adaptation in lizard Phrynocephalus vlangalii living on Qinghai-Tibet plateau". J. Exp. Zool. Part A Vol 319: 117-123.
32. Huang M.Y., Zhang Y.H., Wang H.Y. (2004): "Hematological studies on stream salamander (Batrachuperus tibetanus)". J. Shaanxi Norm. Univ. (Nat. Sci. Edit.) Vol 32: 87-90.
33. Hutchison V., Haines H., Engbretson G. (1976): "Aquatic life at high altitude: respiratory adaptations in the Lake Titicaca frog, Telmatobius culeus". Resp. Physiol. Vol 27: 115-129. [Crossref]
34. iCALIBUR (2014): BioLife Std. Beijing iCALIBUR Research & Development Center.
35. IUCN (2016): The IUCN Red List of Threatened Species. Version 2016-3. Downloaded on 07 December 2016.
36. Jakob E.M., Marshall S.D., Uetz G.W. (1996): "Estimating fitness: a comparison of body condition indices". Oikos Vol 77: 61-67. [Crossref]
37. Jiang Z.G., Jiang J.P., Wang Y.Z., Zhang E., Zhang Y.Y., Li L.L., Xie F., Cai B., Cao L., Zheng G.M., Dong L., Zhang Z.W., Ding P., Luo Z.H., Ding C.Q., Ma Z.J., Tang S.H., Cao W.X., Li C.W., Hu H.J., Ma Y., Wu Y., Wang Y.X., Zhou K.Y., Liu S.Y., Chen Y.Y., Li J.T., Feng Z.J., Wang Y., Wang B., Li C., Song X.L., Cai L., Zang C.X., Zeng Y., Meng Z.B., Fang H.X., Ping X.G. (2016): "Red list of China’s vertebrates". Biodivers. Sci. Vol 24: 500-551. [Crossref]
38. Kaminski P., Jerzak L., Sparks T.H., Johnston A., Bochenski M., Kasprzak M., Wiśniewska E., Mroczkowski S., Tryjanowski P. (2014): "Sex and other sources of variation in the haematological parameters of white stork Ciconia ciconia chicks". J. Ornithol. Vol 155: 307-314. [Crossref]
39. Khan T.A., Zafar F. (2005): "Haematological study in response to varying doses of estrogen in broiler chicken". Int. J. Poul. Sci. Vol 4: 748-751. [Crossref]
40. Koca Y.B., Koca S., Olgun K., Bevtas P., Üzüm N.T. (2006): "Blood cell morphology, erythrocyte size, and micronucleus counts of Neurergus crocatus (Cope, 1862) (Urodela: Salamandridae) in Turkey". Russ. J. Herpetol. Vol 13: 83-88.
41. Kupfer A. (2007): "Sexual size dimorphism in amphibians: an overview". In: Sex, Size and Gender Roles: Evolutionary Studies of Sexual Size Dimorphism, p.  50-60. Fairbairn D.J., Blanchnhorn W.U., Székely T., Eds, Oxford University Press, Oxford. [Crossref]
42. Kuramoto M. (1981): "Relationships between number size and shape of red blood cells in amphibians". Comp. Biochem. Physiol. Part A: Mol. Integr. Physiol. Vol 69: 771-775. [Crossref]
43. Lu S.S., Xin Y., Tang X.L., Yue F., Wang H.H., Bai Y.C., Niu Y.G., Chen Q. (2015): "Differences in hematological traits between high- and low-altitude lizards (genus Phrynocephalus)". PLoS ONE Vol 10: e0125751. [Crossref]
44. Ma D.B. (2005): "Morphological parameter of blood cells in Hynobius leechii Boulenger and Salamandrella keyserlingii". J. Harbin Univ. Vol 26: 123-124.
45. Ma D.B., Wu W.F., Wei H. (2003): "Morphological parameter of blood cells in Hynobius leechii Boulenger and Cynops orientalis David". J. Sci. Teachers’ Coll. Univ. Vol 23: 44-45.
46. Mahapatra B.B., Das M., Dutta S.K., Mahapatra P.K. (2012): "Hematology of Indian rhacophorid tree frog Polypedates maculatus Gray, 1833 (Anura: Rhacophoridae)". Comp. Clin. Pathol. Vol 21: 453-460. [Crossref]
47. Meesawat S., Kitana N., Kitana J. (2016): "Hematology of wild caught Hoplobatrachus rugulosus in northern Thailand". Asian Herpetol. Res. Vol 7: 131-138.
48. Nikinmaa M.M. (1997): "Oxygen and carbon dioxide transport in vertebrate erythrocytes: an evolutionary change in the role of membrane transport". J. Exp. Biol. Vol 200: 369-380.
49. Park D., Park S.-R. (2000): "Multiple insemination and reproductive biology of Hynobius leechii". J. Herpetol. Vol 34: 594-598. [Crossref]
50. Park S.-R., Park D.-S., Yang S.Y. (1996): "Courtship, fighting behaviors and sexual dimorphism of the salamander, Hynobius leechii". Korean J. Zool. Vol 39: 437-446.
51. Raffel T.R., Rohr J.R., Kiesecker J.M., Hudson P.J. (2006): "Negative effects of changing temperature on amphibian immunity under field conditions". Funct. Ecol. Vol 20: 819-828. [Crossref]
52. Ruiz G., Rosenmann M., Nunez H. (1993): "Blood values in South American lizards form high and low altitudes". Comp. Biochem. Physiol. Part A: Mol. Integr. Physiol. Vol 106: 713-718. [Crossref]
53. Ruiz G., Rosenmann M., Veloso A. (1983): "Respiratory and hematological adaptations to high altitude in Telmatobius frogs from the Chilean Andes". Comp. Biochem. Physiol. Part A: Mol. Integr. Physiol. Vol 76: 109-113. [Crossref]
54. Ruiz G., Rosenmann M., Veloso A. (1989): "Altitudinal distribution and blood values in the toad, Bufo spinulosus Wiegmann". Comp. Biochem. Physiol. Part A: Mol. Integr. Physiol. Vol 94: 643-646. [Crossref]
55. Sarasola J.H., Negro J.J., Travaini A. (2004): "Nutritional condition and serum biochemistry for free-living Swainson’s hawks wintering in central Argentina". Comp. Biochem. Physiol. Part A: Mol. Integr. Physiol. Vol 137: 697-701. [Crossref]
56. Seaman D.A., Guglielmo C.G., Williams T.D. (2005): "Effects of physiological state, mass change and diet on plasma metabolite profiles in the western sandpiper Calidris mauri". J. Exp. Biol. Vol 208: 761-769. [Crossref]
57. Shine R. (1979): "Sexual selection and sexual dimorphism in Amphibia". Copeia Vol 1979: 297-306. [Crossref]
58. Sztatecsny M., Schabetsberger R. (2005): "Into thin air: vertical migration, body condition, and quality of terrestrial habitats of alpine common toads, Bufo bufo". Can. J. Zool. Vol 83: 788-796. [Crossref]
59. Tok C.V., Tosunoğlu M., Ayaz D., Çiçek K., Gül Ç. (2009): "Hematology of the Lycian salamander, Lyciasalamandra fazilae". North-West J. Zool. Vol 5: 321-329.
60. Tosunoğlu M., Gül Ç., Uysal İ. (2013): "Hematology of Triturus karelinii (Strauch, 1870) from Gallipoli, Turkey". Herpetozoa Vol 25: 157-159.
61. Tosunoğlu M., Tok C.V., Olgun K., Özdemir N., Gül Ç. (2011): "Hematology of the northern banded newt, Ommatotrition ophryticus (Amphibia: Urodela), from north Anatolia". Russ. J. Herpetol. Vol 18: 59-64.
62. Vaissi S., Parto P., Sharifi M., Haghighi Z.M.S. (2012): "Variations in the size of erythrocytes in the blood of Neurergus kaiseri and Neurergus microspilotus from Iran". Salamandra Vol 48: 193-197.
63. Wang L.W. (1996): "Observation on the morphology of blood cell and test of blood of Hynobius leechii". J. Shengyang Teachers Coll. (Nat. Sci.) Vol 14: 53-57.
64. Waugh A., Grant A. (2001): Anatomy and Physiology in Health and Illness, 9th Edition. Churchill Livingstone.
65. Wyckoff S.M., Frase B.A. (1990): "Hematological adaptation to hypoxia in Peromyscus and Microtus at high and low altitude". T. Illinois State Acad. Sci. Vol 83: 197-205.
66. Xin G.S., Long R.J., Guo X.S., Irvine J., Ding L.M., Ding L.L., Shang Z.H. (2011): "Blood mineral status of grazing Tibetan sheep in the northeast of the Qinghai-Tibetan Plateau". Livest. Sci. Vol 136: 102-107. [Crossref]
67. Ye X.F., Zhang J.J., Yuan L., Wang X.L. (2012): "Study on the hematology of Ranodon sibiricus". Xinjiangxumuye Vol 11: 33-36.
68. Zhang H.J., Xie C.X., Li D.P., Xiong D.M., Liu H.P., Suolang S.Z., Shang P. (2010): "Haematological and blood biochemical characteristics of Glyptosternum maculatum (Siluriformes: Sisoridae) in Xizang (Tibet)". Fish Physiol. Biochem. Vol 36: 797-801. [Crossref]
69. Zhelev Z.M., Mehterov N.H., Popgeorgiev G.S. (2016): "Seasonal changes of basic erythrocyte-metric parameters in Pelophylax ridibundus (Amphibia: Ranidae) from anthropogenically polluted biotopes in southern Bulgaria and their role as bioindicators". Ecotox. Environ. Safe. Vol 124: 406-417. [Crossref]
70. Zhelev Z.M., Popgeorgiev G.S., Mehterov N.H. (2015): "Haematological parameters of Pelophylax ridibundus (Amphibia: Ranidae) from the region of the lead and zinc plant “Kardzhali” (south Bulgaria) and their use in the environmental quality assessment". Acta Zool. Bulg. Vol 67: 271-282.

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