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Low mitochondrial divergence of rediscovered southern relict populations of Rana temporaria parvipalmata in Spain

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We determined DNA sequences of the mitochondrial cytochrome b gene for 44 specimens of the common frog, Rana temporaria parvipalmata, from 14 localities in Galicia, northwestern Spain. We found a low differentiation among all studied individuals, with a maximum of four mutational steps between haplotypes, including those from the southernmost isolated populations on the Manzaneda-Queixa and Trevinca-Segundeira massifs which thus probably originated by a rather recent colonization and likely maintained intermittent gene flow to more northern populations during climatically colder, glacial periods with a higher habitat connectivity.

Affiliations: 1: Departamento de Biología Animal, Biología Vegetal y Ecología, Facultad de Ciencias, Universidad de A Coruña, 15071 A Coruña, Spain; 2: Zoological Institute, Technical University of Braunschweig, Spielmannstr. 8, 38106 Braunschweig, Germany; 3: Zoological Institute, Technical University of Braunschweig, Spielmannstr. 8, 38106 Braunschweig, Germany;, Email:


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