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The genetic crisis of the Mexican Bolson Tortoise (Gopherus flavomarginatus: Testudinidae)

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Gopherus flavomarginatus (Testudinidae) is endemic to a series of discontinuous, isolated basins collectively known as the Bolson de Mapimí in the Chihuahuan Desert. Its numbers declined after catastrophic levels of exploitation during the mid-20th century. However currently, the Bolson Tortoise appears to be on a path to recovery owing to intensive, sustained conservation efforts. We sequenced an 842-bp-long fragment of the D-loop from 76 individuals distributed throughout the species’ range. The results revealed only two haplotypes. An AMOVA showed that 95% of the variance occurred among populations, whereas the remaining 5% was explained by genetic differences within populations. Tectonic processes together with ecological transformation during the Pleistocene and Holocene may be responsible for the reduction in this species’ genetic variation. A bottleneck during which a significant percentage of the haplotype diversity was lost would result in genetic homogeneity. Although there is demographic growth, the lack of genetic diversity is indicative of the potential crisis that the Bolson Tortoise is facing, and awareness must be brought to this situation.

Affiliations: 1: Laboratorio de Sistemática Filogenética, Departamento de Biología Evolutiva, Instituto de Ecología, A. C., Carretera antigua a Coatepec No. 351, Xalapa, Veracruz 91070, México

*Corresponding author; e-mail: alejandro.espinosa@inecol.edu.mx
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1. Aguirre G. , Morafka D.J. , Adest G.A. (1997): "Conservation strategies for the Bolson Tortoise, Gopherus flavomarginatus, in the Chihuahuan Desert". In: Proceedings: Conservation, Restoration, and Management of Tortoises and Turtles – An International Conference, p.  333- 338. van Abbema J. , Ed., New York Turtle and Tortoise Society.
2. Alderman R. , Double M.C. , Valencia J. , Gales R.P. (2005): "Genetic affinities of newly sampled populations of wandering and black-browed albatross". Emu Vol 105: 169- 179. http://dx.doi.org/10.1071/MU04034
3. Amato M.L. , Brooks R.J. , Fu J. (2008): "A phylogeographic analysis of populations of the wood turtle (Glyptemys insculpta) throughout its range". Mol. Ecol. Vol 17: 570- 581.
4. Avise J.C. (2000): Phylogeography: The History and Formation of Species. Harvard University Press, Cambridge, MA.
5. Avise J.C. , Bowen B.W. , Lamb T. , Meylan A.B. , Bermingham E. (1992): "Mitochondrial DNA evolution at a turtle’s pace: evidence for low genetic variability and reduced microevolutionary rate in the Testudines". Mol. Biol. Evol. Vol 9: 457- 473.
6. Baxter B.D. , Mendez-Harclerode F.M. , Fulhorst C.F. , Bradley R.D. (2009): "A molecular examination of relatedness, multiple paternity, and cohabitation of the Southern Plains woodrat (Neotoma micropus)". J. Mammal. Vol 90: 819- 831. http://dx.doi.org/10.1644/08-MAMM-A-195.1
7. Beheregaray L.B. , Ciofi C. , Caccone A. , Gibbs J.P. , Powell J.R. (2003): "Genetic divergence, phylogeography and conservation units of giant tortoises from Santa Cruz and Pinzón, Galápagos Islands". Conserv. Genet. Vol 4: 31- 46. http://dx.doi.org/10.1023/A:1021864214375
8. Bourjea J. , Lapegue S. , Gagnevin L. , Broderick D. , Mortimer J.A. , Ciccione S. , Roos D. , Taquet C. , Grizel H. (2007): "Phylogeography of the green turtle, Chelonia mydas, in the Southwest Indian Ocean". Mol. Ecol. Vol 16: 175- 186. http://dx.doi.org/10.1111/j.1365-294X.2006.03122.x
9. Bowen B.W. , Abreu-Grobois F.A. , Balazs G.H. , Kamezaki N. , Limpus C.J. , Ferl R.J. (1995): "Trans-Pacific migrations of the loggerhead turtle (Caretta caretta) demonstrated with mitochondrial DNA markers". Proc. Natl. Acad. Sci. USA Vol 92: 3731- 3734. http://dx.doi.org/10.1073/pnas.92.9.3731
10. Bowen B.W. , Clark A.M. , Abreu-Grobois F.A. , Chaves A. , Reichart H.A. , Ferl R.J. (1998): "Global phylogeography of the Ridley sea turtles (Lepidochelys spp.) as inferred from mitochondrial DNA sequences". Genetica Vol 101: 179- 189. http://dx.doi.org/10.1023/A:1018382415005
11. Chassin-Noria O. , Abreu-Grobois A. , Dutton P.H. , Oyama K. (2004): "Conservation genetics of the east Pacific green turtle (Chelonia mydas) in Michoacan, Mexico". Genetica Vol 121: 195- 206. http://dx.doi.org/10.1023/B:GENE.0000040394.47843.e4
12. Echelle A.A. , Hackler J.C. , Lack J.B. , Ballard S.R. , Roman J. , Fox S.F. , Leslie D.M. , van den Bussche R.A. (2010): "Conservation genetics of the alligator snapping turtle: cytonuclear evidence of range-wide bottleneck effects and unusually pronounced geographic structure". Conserv. Genet. Vol 11: 1375- 1387. http://dx.doi.org/10.1007/s10592-009-9966-1
13. Encalada S.E. , Lahanas P.N. , Bjorndal K.A. , Bolten A.B. , Miyamoto M.M. , Bowen B.W. (1996): "Phylogeography and population structure of the Atlantic and Mediterranean green turtle Chelonia mydas: a mitochondrial DNA control region sequence assessment". Mol. Ecol. Vol 5: 473- 483. http://dx.doi.org/10.1111/j.1365-294X.1996.tb00340.x
14. Excoffier L.G.L. , Schneider S. (2005): "Arlequin ver. 3.0: An integrated software package for population genetics data analysis". Evol. Bioinf. Online Vol 1: 47- 50.
15. Excoffier L. , Smouse P. , Quattro J. (1992): "Analysis of molecular variance inferred from metric distances among DNA haplotypes: Application to human mitochondrial DNA restriction data". Genetics Vol 131: 479- 491.
16. Fleming T.H. , Valiente-Banuet A. (2002): Columnar Cacti and Their Mutualists: Evolution, Ecology, and Conservation. University of Arizona Press, Tucson, AZ.
17. Formia A. , Godley B.J. , Dontaine J.F. , Bruford M.W. (2006): "Mitochondrial DNA diversity and phylogeography of endangered Green turtle (Chelonia mydas) populations in Africa". Conserv. Genet. Vol 7: 353- 369. http://dx.doi.org/10.1007/s10592-005-9047-z
18. Frankham R. (1995): "Conservation genetics". Annu. Rev. Gent. Vol 29: 305- 327. http://dx.doi.org/10.1146/annurev.ge.29.120195.001513
19. Frankham R. , Ballou J.D. , Briscoe D.A. (2002): Introduction to Conservation Genetics. Cambridge University Press, Cambridge. http://dx.doi.org/10.1017/CBO9780511808999
20. Fritz U. , Daniels S.R. , Hofmeyr M.D. , González J. , Barrio-Amorós C.L. , Široký P. , Hundsdörfer A.K. , Stuckas H. (2010): "Mitochondrial phylogeography and subspecies of the wide-ranging sub-Saharan leopard tortoise Stigmochelys pardalis (Testudines: Testudinidae) – a case study for the pitfalls of pseudogenes and GenBank sequences". J. Zool. Syst. Evol. Res. Vol 48: 348- 359. http://dx.doi.org/10.1111/j.1439-0469.2010.00565.x
21. Fujii A. , Forstner R.J. (2010): "Genetic variation and population structure of the Texas Tortoise, Gopherus berlandieri (Testudinidae), with implications for conservation. Chelon". Conserv. Biol. Vol 9: 61- 69.
22. González T.R. , Aguirre G. (2006): "La tortuga del Bolsón, Gopherus flavomarginatus ". Reptilia Vol 62: 26- 33.
23. Jian S. , Ban J. , Ren H. , Yan H. (2010): "Low genetic variation detected within the widespread mangrove species Nypa fruticans (Palmae) from Southeast Asia". Aquat. Bot. Vol 92: 23- 27. http://dx.doi.org/10.1016/j.aquabot.2009.09.003
24. Leakey R. , Lewin R. (1995): The Sixth Extinction: Biodiversity and Its Survival. Doubleday, New York.
25. Librado P. , Rosas J. (2009): "DnaSP v5: a software for comprehensive analysis of DNA polymorphism data". Bioinformatics Vol 25: 1451- 1452. http://dx.doi.org/10.1093/bioinformatics/btp187
26. Lidicker W.Z. , Koening W.D. (1996): "Responses of terrestrial vertebrates to habitat edges and corridors". In: Metapopulation and Wildlife Conservation, p. 85- 109. McCullough D.R. , Ed., Island Press, Washington, DC.
27. Lynch M. , Crease T.J. (1990): "The analysis of population survey data on DNA sequence variation". Mol. Biol. Evol. Vol 7: 377- 394.
28. Milot E. , Weimerskirch H. , Duchesne P. , Bernatchez L. (2007): "Surviving with low genetic diversity: the case of albatrosses". Proc. Biol. Sci. Vol 274: 779- 787. http://dx.doi.org/10.1098/rspb.2006.0221
29. Morafka D.J. (1977): "A biogeographical analysis of the Chihuahuan desert through its herpetofauna". Biogegraphica Vol 9: 1- 317.
30. Morafka D.J. (1982): "The status and distribution of the Bolson Tortoise, Gopherus flavomarginatus ". In: North American Tortoises: Conservation and Ecology, p. 71- 94. Bury R.B. , Ed., Fish and Wildlife Service Wildlife Research Report 12. Washington, DC.
31. Morafka D.J. (1988): "Part III. Historical biogeography of the Bolson Tortoise". Ann. Carn. Mus. Vol 57: 47- 72.
32. Morafka D.J. , Aguirre L.G. , Adest G.A. (1989): " Gopherus flavomargintus Bolson Tortoise". In: The Conservation Biology of Tortoises, p. 10- 13. Swingland I.R. , Klemens M.W. , Eds, Occassional Paper IUCN Species Survival Commission 5. Gland, Switzerland.
33. Morafka D.J. , Aguirre L.G. , Murphy R.W. (1994): "Allozyme differentiation among gopher tortoises (Gopherus): Conservation genetics and phylogenetic and taxonomic implications". Can. J. Zool. Vol 72: 1665- 1671. http://dx.doi.org/10.1139/z94-222
34. Olson D.M. , Dinerstein E. (1998): "The Global 200: a representation approach to conserving the earth’s most biologically valuable ecoregions". Conserv. Biol. Vol 3: 502- 512. http://dx.doi.org/10.1046/j.1523-1739.1998.012003502.x
35. Pearse D.E. , Arndt A.D. , Valenzuela N. , Miller B.A. , Cantarelli V. , Sites J.R. (2006): "Estimating population structure under nonequilibrium conditions in a conservation context: continent-wide population genetics of the giant Amazon River turtle, Podocnemis expansa (Chelonia; Podocnemididae)". Mol. Ecol. Vol 15: 985- 1006. http://dx.doi.org/10.1111/j.1365-294X.2006.02869.x
36. Peterson A.T. , Ortega-Huerta M.A. , Bartley J. , Sánchez-Cordero V. , Soberón J. , Buddemeier R.H. , Stockwell D.R.B. (2002): "Future projections for Mexican faunas under global climate change scenarios". Nature Vol 416: 626- 629. http://dx.doi.org/10.1038/416626a
37. Reis E.C. , Albano R.M. , Bondioli A.C.V. , Soares L.S. , Lôbo-Hajdu G. (2009): "Detection of polymorphisms of the mtDNA control region of Caretta caretta (Testudines: Cheloniidae) by PCR-SSCP". GMR, Genet. Mol. Res. Vol 8: 215- 222. http://dx.doi.org/10.4238/vol8-1gmr570
38. Roman J. , Santhuff S.D. , Moler P.E. , Bowen B.W. (1999): "Population structure and cryptic evolutionary units in the Alligator snapping turtle". Conserv. Biol. Vol 13: 135- 142. http://dx.doi.org/10.1046/j.1523-1739.1999.98007.x
39. Rosenbaum P.A. , Robertson J.M. , Zamudio K.R. (2007): "Unexpectedly low genetic divergences among populations of the threatened Bog turtle (Glyptemys muhlenbergii)". Conserv. Genet. Vol 8: 331- 342. http://dx.doi.org/10.1007/s10592-006-9172-3
40. Sbisa E. , Tanzariellob F. , Reyesa A. , Pesolec G. , Saccone C. (1997): "Mammalian mitochondrial D-loop region structural analysis: Identification of new conserved sequences and their functional and evolutionary implications". Gene Vol 205: 125- 140. http://dx.doi.org/10.1016/S0378-1119(97)00404-6
41. Schultz J.K. , Baker J.D. , Toonen R.J. , Bowen B.W. (2009): "Extremely low genetic diversity in the endangered Hawaiian Monk seal (Monachus schauinslandi)". J. Hered. Vol 100: 25- 33. http://dx.doi.org/10.1093/jhered/esn077
42. Singer-Sam J. , Tanguay R.L. , Riggs A.D. (1989): "Use of Chelex to improve PCR signals from small number of cells". Amplifications (A forum for PCR users) Vol 3: 11.
43. Sites J.W. , FitzSimmons N.N. , Da Silva N.J. , Cantarelli V.H. (2001). "Conservation genetics of the Giant Amazon River turtle (Podocnemis expansa; Pelomedusidae): Inferences from two classes of molecular markers". Chelon. Conserv. Biol. Vol 3: 454- 463.
44. Starkey D.E. , Shaffer H.B. , Burke R.L. , Forstner M.R.J. , Iverson J.B. , Janzen F.J. , Rhodin A.G.J. , Ultsch G.R. (2003): "Molecular systematics, phylogeography, and the effects of Pleistocene glaciations in the Painted turtle (Chrysemys picta) complex". Evolution Vol 57: 119- 128. http://dx.doi.org/10.1111/j.0014-3820.2003.tb00220.x
45. Souza F.L. , Cunha A.F. , Oliveira M.A. , Pereira G.A.G. , Dos Reis S.F. (2003). "Preliminary phylogeographic analysis of the neotropical freshwater turtle Hydromedusa maximiliani (Chelidae)". J. Herpetol. Vol 37: 427- 433. http://dx.doi.org/10.1670/0022-1511(2003)037[0427:PPAOTN]2.0.CO;2
46. Vanbrabant J. , Goddeeris B. , Martens K. , Schön I. (2009): "Development of primers for amplification and further research on the D-loop control region for the Common Spadefoot toad, Pelobates fuscus (Amphibia)". Conserv. Genet. Resour. Vol 1: 495- 499. http://dx.doi.org/10.1007/s12686-009-9115-7
47. van der Kuyl A.C. , Ballasina D.L.P. , Zorgdrager F. (2005): "Mitochondrial haplotype diversity in the tortoise species Testudo graeca from North Africa and the Middle East". BMC Evol. Biol. Vol 5: 29. http://dx.doi.org/10.1186/1471-2148-5-29
48. van Dijk P.P. , Flores-Villela O. (2007): Gopherus flavomarginatus. In: IUCN. 2011. IUCN Red List of Threatened Species. . [Downloaded on 12 September 2011.]
49. Vargas-Ramírez M. , Stuckas H. , Castaño-Mora O.V. , Fritz U. (2011). "Extremely low genetic diversity and weak population differentiation in the endangered Colombian river turtle Podocnemis lewyana (Testudines: Podocnemididae)". Conserv. Genet. On-line first. DOI 10.1007/s010592-011-0263-4.
50. Velo-Antón G. , Cordero-Rivera A. , García-París M. (2008): "Patterns of nuclear and mitochondrial DNA variation in Iberian populations of Emys orbicularis (Emydidae): conservation implications". Conserv. Genet. Vol 9: 1263- 1274. http://dx.doi.org/10.1007/s10592-007-9457-1
51. Walker D. , Avise J.C. (1998): "Principles of phylogeography as illustrated by freshwater and terrestrial turtles in the southeastern United States". Annu. Rev. Ecol. Syst. Vol 29: 23- 58. http://dx.doi.org/10.1146/annurev.ecolsys.29.1.23
52. Walker D. , Ortí G. , Avise J.C. (1998): "Phylogenetic distinctiveness of a threatened aquatic turtle (Sternotherus depressus)". Conserv. Biol. Vol 12: 639- 645. http://dx.doi.org/10.1046/j.1523-1739.1998.97056.x
53. Walker D. , Moler P.E. , Buhlmann K.A. , Avise J.C. (1998): "Phylogeographic uniformity in mitochondrial DNA of the Snapping turtle (Chelydra serpentina)". Anim. Conserv. Vol 1: 55- 60. http://dx.doi.org/10.1111/j.1469-1795.1998.tb00226.x
54. Zhong J. , Liu Z.Q. , Wang Y.Q. (2008): "Phylogeography of the Rice Frog, Fejervarya multistriata (Anura: Ranidae), from China based on mtDNA D-loop sequences". Zool. Sci. Vol 25: 811- 820. http://dx.doi.org/10.2108/zsj.25.811
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2012-01-01
2016-12-10

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