Cookies Policy

This site uses cookies. By continuing to browse the site you are agreeing to our use of cookies.

I accept this policy

Find out more here

Development of behavioural and physiological assays to assess discrimination of male and female odours in crayfish, Pacifastacus leniusculus

No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
The full text of this article is not currently available.

Brill’s MyBook program is exclusively available on BrillOnline Books and Journals. Students and scholars affiliated with an institution that has purchased a Brill E-Book on the BrillOnline platform automatically have access to the MyBook option for the title(s) acquired by the Library. Brill MyBook is a print-on-demand paperback copy which is sold at a favorably uniform low price.

Access this article

+ Tax (if applicable)
Add to Favorites
You must be logged in to use this functionality

image of Behaviour

Many aquatic organisms use chemical signals to coordinate courtship. However, relatively few water-borne pheromones have been identified. A key obstacle hindering progress in the purification of crustacean pheromones has been the development of reliable bioassays. This study focuses on developing novel bioassays to guide the purification of sex pheromones in signal crayfish, Pacifastacus leniusculus. We aimed to elicit specific sexual behaviours in male crayfish in response to female urine or conditioned water released from a female dummy. A physiological assay, based on male heartbeat recordings, was developed to assess if physiological tests could provide quicker and more sensitive responses than the behavioural assay. Males exposed to female urine showed significantly increased levels of specific mounting behaviours in comparison to male urine or control water. However, other sexual behaviours such as seizing, turning and spermatophore deposition were not observed. The physiological assay demonstrated that a rapid change in the heart rate of male crayfish could be induced through exposure to odour from conspecific female crayfish.

Our study indicates male crayfish can discriminate between male and female odours. Physiological measures provide a quick assay for sensitivity to a substance whilst behavioural assays indicate its functional significance.

Affiliations: 1: Biological Sciences, University of Hull, Hull HU6 7RX, UK;, Email:; 2: Biological Sciences, University of Hull, Hull HU6 7RX, UK


Full text loading...


Data & Media loading...

Article metrics loading...



Can't access your account?
  • Tools

  • Add to Favorites
  • Printable version
  • Email this page
  • Subscribe to ToC alert
  • Get permissions
  • Recommend to your library

    You must fill out fields marked with: *

    Librarian details
    Your details
    Why are you recommending this title?
    Select reason:
    Behaviour — Recommend this title to your library
  • Export citations
  • Key

  • Full access
  • Open Access
  • Partial/No accessInformation