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RAPD AND AFLP TECHNIQUES FOR THE ANALYSIS OF GENETIC RELATIONSHIPS IN TWO GENERA OF DECAPODA

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[RAPD was used for analysing three (sub-)species of mitten crabs (Eriocheir sinensis, E. japonicus, and E. japonicus hepuensis) and three populations of E. sinensis. The results show that their relationships on DNA level are similar to the classical taxonomic hypotheses (Dai, 1991). No diagnostic RAPD marker could be found, but there were statistically significant genetic differences among these taxa (P < 0.001) or populations (P < 0.001). That is, the intraspecific similarities were larger than the interspecific similarities; the intrasubspecific similarities were larger than the intraspecific similarities; and the intrapopulational similarities were larger than the interpopulational similarities. In AFLP analysis, no significant genetic difference has been found between E. sinensis and E. japonicus, but AFLP markers among four species of Macrobrachium (M. rosenbergii, M. nipponense, M. hainanense, and M. asperulum) were found. The DNA similarities among these four species of Macrobrachium are in accordance with morphological similarities.

RAPD a été utilisé pour analyser trois (sous-)espèces de crabes du genre Eriocheir (E. sinensis, E. japonicus et E. japonicus hepuensis) et trois populations de E. sinensis. Les résultats montrent que leurs relations au niveau du DNA correspondent aux hypothèses taxonomiques classiques (Dai, 1991). Aucun marqueur diagnostique RAPD n'a pu être trouvé, mais il y avait des différences génétiques statistiquement significatives parmi les taxons (P < 0,001) ou les populations (P < 0,001). Ainsi, les similarités intraspécifiques étaient plus grandes que les similarités interspécifiques; les similarités intrasubspécifiques étaient plus grandes que les similarités intersubspécifiques et les similarités intrapopulations étaient plus grandes que les similarités interpopulations. Dans l'analyse AFLP, aucune différence génetique significative n'a été trouvée entre E. sinensis et E. japonicus, mais des marqueurs AFLP sur quatre espèces de Macrobrachium (M. rosenbergii, M. nipponense, M. hainanense, et M. asperulum) ont été trouvés. Les similarités du DNA entre les quatre espèces de Macrobrachium sont en accord avec les similarités morphologiques., RAPD was used for analysing three (sub-)species of mitten crabs (Eriocheir sinensis, E. japonicus, and E. japonicus hepuensis) and three populations of E. sinensis. The results show that their relationships on DNA level are similar to the classical taxonomic hypotheses (Dai, 1991). No diagnostic RAPD marker could be found, but there were statistically significant genetic differences among these taxa (P < 0.001) or populations (P < 0.001). That is, the intraspecific similarities were larger than the interspecific similarities; the intrasubspecific similarities were larger than the intraspecific similarities; and the intrapopulational similarities were larger than the interpopulational similarities. In AFLP analysis, no significant genetic difference has been found between E. sinensis and E. japonicus, but AFLP markers among four species of Macrobrachium (M. rosenbergii, M. nipponense, M. hainanense, and M. asperulum) were found. The DNA similarities among these four species of Macrobrachium are in accordance with morphological similarities.

RAPD a été utilisé pour analyser trois (sous-)espèces de crabes du genre Eriocheir (E. sinensis, E. japonicus et E. japonicus hepuensis) et trois populations de E. sinensis. Les résultats montrent que leurs relations au niveau du DNA correspondent aux hypothèses taxonomiques classiques (Dai, 1991). Aucun marqueur diagnostique RAPD n'a pu être trouvé, mais il y avait des différences génétiques statistiquement significatives parmi les taxons (P < 0,001) ou les populations (P < 0,001). Ainsi, les similarités intraspécifiques étaient plus grandes que les similarités interspécifiques; les similarités intrasubspécifiques étaient plus grandes que les similarités intersubspécifiques et les similarités intrapopulations étaient plus grandes que les similarités interpopulations. Dans l'analyse AFLP, aucune différence génetique significative n'a été trouvée entre E. sinensis et E. japonicus, mais des marqueurs AFLP sur quatre espèces de Macrobrachium (M. rosenbergii, M. nipponense, M. hainanense, et M. asperulum) ont été trouvés. Les similarités du DNA entre les quatre espèces de Macrobrachium sont en accord avec les similarités morphologiques.]

Affiliations: 1: Institute of Hydrobiology, The Chinese Academy of Sciences, Luojiashan, Wuhan 430072, P. R. China

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/content/journals/10.1163/156854000505056
2000-11-01
2016-08-25

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