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Application of RFLP analysis to identify cyst populations of Artemia urmiana Günther, 1899 (Branchiopoda, Anostraca) from Urmia Lake, Iran

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A rapid and reliable PCR-RFLP method was optimized to identify cyst batches of Artemia urmiana collected from different regions of Urmia Lake. Following DNA extraction, a 1564 bp region of a mitochondrial gene encoding the ribosomal RNA was successfully amplified by the PCR technique. Eleven restriction endonucleases were subsequently employed in order to digest the PCR product. These enzymes gave specific restriction patterns for discriminating cyst batches collected from top and bottom layers of three main geographical areas of Urmia Lake. Detailed analysis revealed that the RFLP patterns of four restriction enzymes (HinfI, TaqI, MspI, and MboI) were sufficient to differentiate between the samples studied. The method described is sensitive, rapid, and reliable, and can be a useful tool for the identification of various populations of A. urmiana existing in the ecosystem of Urmia Lake. Une méthode rapide et fiable de PCR-RFLP a été optimisée pour identifier les œufs de durée d'Artemia urmiana récoltés dans différentes régions du lac Urmia. Après extraction du DNA, une région de 1564 bp du gène mitochondrial codant pour le RNA mitochondrial a été amplifiée avec succès par la technique de PCR. Onze endonucléases de restriction ont été ensuite utilisées afin de digérer le produit de la PCR. Ces enzymes ont donné des patrons de restriction spécifiques permettant de discriminer les œufs de durée collectés dans les couches supérieure et inférieure de trois principales régions géographiques du lac Urmia. L'analyse détaillée a révélé que les patrons RFLP de quatre enzymes de restriction (HinfI, TaqI, MspI et MboI) suffisaient pour distinguer les échantillons étudiés. La méthode décrite est sensible, rapide et fiable, et peut être un outil utile pour l'identification des diverses populations de A. urmiana présentes dans l'écosystème du lac Urmia.


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