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Genomic DNA hybridization as an attempt to evaluate phylogenetic relationships of Ostracoda

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Molecular distances obtained by measuring similarity of large DNA fragments using DNA hybridization procedures are an alternative to distances based on sequences of particular genes or their products. However, when studying small animals, the low amount of DNA that can be isolated from a single individual may be a serious problem. Here we present a modified DNA hybridization method, applicable when only a small amount of DNA is available. We use the hybridization data to reconstruct the phylogeny of five species representing three extant superfamilies of Ostracoda, consistent with the traditional frame of classification. We compare three methods of internal branch testing (the bootstrap, the Dopazo test, and the Weighted Least Squares Likelihood Ratio Test) to assess the statistical significance of the results. Our analysis indicates that the total DNA hybridization technique may be useful in preliminary studies of taxonomic relationships between species of small animals. Les distances moléculaires obtenues par la mesure de la similarité de l'ADN par hybridation de grands fragments d'ADN constituent une alternative aux distances évaluées par les séquences de gènes particuliers ou de leurs produits. Cependant, si l'étude concerne des animaux de petite taille, la faible quantité d'ADN isolable à partir d'un seul individu peut constituer un handicap. Nous exposons ici une méthode modifiée d'hybridation de l'ADN, applicable lorsque seule une faible quantité d'ADN est disponible. Nous utilisons les données d'hybridation pour reconstruire la phylogénie de cinq espèces représentant trois vastes superfamilles d'Ostracodes, en accord avec le cadre traditionnel de la classification. Nous comparons trois méthodes d'essai de validation des branchements (le "bootstrap", le test Dopazo et le test de vraisemblance des moindres carrés) pour évaluer la significativité des résultats. Notre analyse montre que la technique d'hybridation totale de l'ADN peut être utile pour des études préliminaires de relations taxonomiques entre espèces de petits animaux.


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