Cookies Policy
X
Cookie Policy

This site uses cookies. By continuing to browse the site you are agreeing to our use of cookies.

I accept this policy

Find out more here

Aspects of mineralization of the cuticle of the crayfish, Procambarus clarkii (Decapoda, Cambaridae)

No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
MyBook is a cheap paperback edition of the original book and will be sold at uniform, low price.

Buy this article

Price:
$30.00+ Tax (if applicable)
Add to Favorites

image of Crustaceana

In the freshwater crayfish, Procambarus clarkii (Girard, 1852), histological cuticle sections treated with 1° antibodies to CA (carbonic anhydrase) stained more intensely along the matrix fibrils than controls exposed only to the 2° antibodies and to the phosphatase substrate. The poorly calcified and more flexible ventral abdominal cuticle only stained weakly. Mineralization was greatest in the strongly staining cuticle from the dorsal carapace (61%). In the weakly staining ventral abdominal cuticle mineralization was much less (4.5%). Magnesium and strontium carbonates were of little importance in mineralization. Dot blots of water soluble proteins extracted via EDTA (Ethylenediaminetetraaceticacid) and affinity chromatography from crayfish cuticles also responded positively to 1° antibodies to bovine RBC (red blood corpusle) CA. Chez l'écrevisse Procambarus clarkii (Girard, 1852), des coupes histologiques de cuticule traitées avec un anticorps anti-anhydrase carbonique se colorent plus intensément le long de la matrice des fibrilles que chez les coupes témoins exposées seulement au 2ème anticorps et au substrat. La cuticule ventrale de l'abdomen, pauvrement calcifiée et plus flexible se colore seulement faiblement. La minéralisation est la plus importante au niveau de la carapace dorsale (61%). Au niveau de la cuticule ventrale abdominale faiblement colorée, la minéralisation est plus faible (4,5%). Les carbonates de magnésium et de strontium sont de faible importance dans la minéralisation. Les dots blots des protéines hydrosolubles extraites par l'EDTA et la chromatographie d'affinité de cuticules d'écrevisses répondent aussi positivement à l'anticorps anti-CA d'érythrocyte bovin.

Loading

Article metrics loading...

/content/journals/10.1163/156854009x452740
2009-08-01
2015-05-27

Affiliations: 1: Department of Biology, Texas State University, 601 University Drive, San Marcos, Texas, U.S.A.; 2: Department of Biology, Worcester State College, 486 Chandler Street, Worcester, Massachusetts, U.S.A.; 3: Division of Natural Sciences, College of General Studies, Boston University, 871 Commonwealth Avenue, Boston, Massachusetts, U.S.A.

Sign-in

Can't access your account?
  • Tools

  • Add to Favorites
  • Printable version
  • Email this page
  • Subscribe to email alerts
  • Get permissions
  • Recommend to your library

    You must fill out fields marked with: *

    Librarian details
    Name:*
    Email:*
    Your details
    Name:*
    Email:*
    Department:*
    Why are you recommending this title?
    Select reason:
     
     
     
     
    Other:
     
    Crustaceana — Recommend this title to your library

    Thank you

    Your recommendation has been sent to your librarian.

  • Export citations
  • Key

  • Full access
  • Open Access
  • Partial/No accessInformation