Cookies Policy
X
Cookie Policy

This site uses cookies. By continuing to browse the site you are agreeing to our use of cookies.

I accept this policy

Find out more here

Primary Cell Culture and Characteristic Morphologies of Neurons in the Eyestalks of the Kuruma Shrimp, Marsupenaeus Japonicus (Decapoda, Penaeoidea)

No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
MyBook is a cheap paperback edition of the original book and will be sold at uniform, low price.

Buy this article

Price:
$30.00+ Tax (if applicable)
Add to Favorites

image of Crustaceana

In the present study, neurons isolated from the eyestalks of the Kuruma shrimp, Marsupenaeus japonicus were successfully cultured in vitro. The cultured neurons could be assigned to five different categories according to initial cell viability and the subsequent growth potential. They were “multipolar cells”, “bipolar cells”, “unipolar cells”, “veilers”, and “large cells”. Most neurons were kept alive for more than 7 days. The most common cells in the culture were the bipolar cells, which survived up to two weeks. The neurons isolated by collagenase (type I) or papain had a similar number of alive cells and a similar survival time. The neurons were adapted to modified Medium 199 and Liebowitz's-15 medium, which were widely used in aquatic cell culture. A muscle extract from the Kuruma shrimp inhibited the outgrowth of neurons, while the survival period of neurons was extended for 4 days in media enriched with 20% foetal bovine serum. Our study provides valuable methods for further research on the electrophysiology of these neurons in vitro.

Loading

Article metrics loading...

/content/journals/10.1163/156854011x608465
2011-12-01
2015-03-03

Affiliations: 1: College of Oceanography and Environmental Science, Xiamen University, Siming South Road, Xiamen, 361005, China;, Email: haihuiye@xmu.edu.cn; 2: College of Oceanography and Environmental Science, Xiamen University, Siming South Road, Xiamen, 361005, China

Sign-in

Can't access your account?
  • Tools

  • Add to Favorites
  • Printable version
  • Email this page
  • Subscribe to email alerts
  • Get permissions
  • Recommend to your library

    You must fill out fields marked with: *

    Librarian details
    Name:*
    Email:*
    Your details
    Name:*
    Email:*
    Department:*
    Why are you recommending this title?
    Select reason:
     
     
     
     
    Other:
     
    Crustaceana — Recommend this title to your library

    Thank you

    Your recommendation has been sent to your librarian.

  • Export citations
  • Key

  • Full access
  • Open Access
  • Partial/No accessInformation