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Isolation of the C-type lectin like-domain cDNAs from the mud crab Scylla paramamosain Estampador, 1949, and its expression profiles in various tissues, during larval development, and under Vibrio challenge

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Scylla paramamosain Estampador, 1949 is one of the most precious marine crabs farmed in China. The crab is prone to infection by microbes at various stages during its growth, leading to high mortality. C-type lectin has a characteristic carbohydrate recognition domain and plays an important role in the immunity system. A cDNA library from the mud crab S. paramamosain was constructed using the SMART technique. Two complete cDNA sequences showing high identities with the C-type lectin gene were isolated from the library. The two full-length C-type lectin cDNAs of S. paramamosain (SpLec1 and SpLec2) consist of 746 and 834 bp, respectively. Quantitative real-time PCR analyses revealed that the C-type lectins were expressed mainly in haemocytes, muscle and hepatopancreas, with the highest expression level found in hepatopancreas. The C-type lectin mRNA expression in continuous developmental stages during zoeal phases were also examined by quantitative real-time PCR. After infection with Vibrio parahaemolyticus (Sakazaki, 1963) at a concentration of 2.3 × 106 cfu/ml, the temporal expression of SpLec1 and SpLec2 mRNA in the megalopa stage was first increased, reached a maximum, and then dropped to the original level again. The research of C-type lectins in Scylla paramamosain could shed new light on studies on immunity and moulting in the mud crab.

Affiliations: 1: 1East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 300 Jungong Road, Shanghai 200090, P.R. China

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1. Arnold K. , Bordoli L. , Kopp J. , Schwede T. , 2006. "The SWISS-MODEL Workspace: a web-based environment for protein structure homology modelling". Bioinformatics, Vol 22: 195- 201. http://dx.doi.org/10.1093/bioinformatics/bti770
2. Chai L.-Q. , Tian Y.-Y. , Yang D.-T. , Wang J.-X. , Zhao X.-F. , 2008. "Molecular cloning and characterization of a C-type lectin from the cotton bollworm, Helicoverpa armigera ". Dev. Comp. Immunol., Vol 32: 71- 83. http://dx.doi.org/10.1016/j.dci.2007.04.006
3. Dodd R. B. , Drickamer K. , 2001. "Lectin-like proteins in model organisms: implications for evolution of carbohydrate-binding activity". Glycobiology, Vol 11: 71- 79. http://dx.doi.org/10.1093/glycob/11.5.71R
4. Gross P. S. , Bartlett T. C. , Browdy C. L. , Chapman R. W. , Warr G. W. , 2001. "Immune gene discovery by expressed sequence tag analysis of hemocytes and hepatopancreas in the Pacific White Shrimp, Litopenaeus vannamei, and the Atlantic white shrimp, L. setiferus ". Dev. Comp. Immunol., Vol 25: 565- 577. http://dx.doi.org/10.1016/S0145-305X(01)00018-0
5. Hosono M. , Sugawara S. , Ogawa Y. , Kohno T. , Takayanagi M. , Nitta K. , 2005. "Purification, characterization, cDNA cloning, and expression of asialofetuin-binding C-type lectin from eggs of Shishamo smelt ( Osmerus[ Spirinchus] lanceolatus)". Biochim. Biophys. Acta General Subjects, Vol 1725: 160- 173. http://dx.doi.org/10.1016/j.bbagen.2005.07.009
6. Imai H. , Takeda M. , 2005. "A natural hybrid mud crab (Decapoda, Portunidae) from Japan". Journ. Crustacean Biol., Vol 25: 620- 624. http://dx.doi.org/10.1651/C-2493.1
7. Imjongjirak C. , Amparyup P. , Tassanakajon A. , Sittipraneed S. , 2007. "Antilipopolysaccharide factor (ALF) of the mud crab Scylla paramamosain: molecular cloning, genomic organization and the antimicrobial activity of its synthetic LPS binding domain". Mol. Immunol., Vol 44: 3195- 3203. http://dx.doi.org/10.1016/j.molimm.2007.01.028
8. Kawaguchi N. , Komano H. , Natori S. , 1991. "Involvement of Sarcophagalectin in the development of imaginal discs of Sarcophaga peregrinain an autocrine manner". Dev. Biol., Vol 144: 86- 93. http://dx.doi.org/10.1016/0012-1606(91)90481-H
9. Kong H. J. , Park E.-M. , Nam B.-H. , Kim Y.-O. , Kim W.-J. , Park H.-J. , Lee C. H. , Lee S.-J. , 2008. "A C-type lectin like-domain (CTLD)-containing protein (PtLP) from the swimming crab Portunus trituberculatus ". Fish Shellfish Immunol., Vol 25: 311- 314. http://dx.doi.org/10.1016/j.fsi.2008.05.003
10. Liu Y.-C. , Li F.-H. , Dong B. , Wang B. , Luan W. , Zhang X.-J. , Zhang L.-S. , Xiang J.-H. , 2007. "Molecular cloning, characterization and expression analysis of a putative C-type lectin (Fclectin) gene in the Chinese shrimp Fenneropenaeus chinensis ". Mol. Immunol., Vol 44: 598- 607. http://dx.doi.org/10.1016/j.molimm.2006.01.015
11. Luo T. , Yang H. , Li F. , Zhang X. , Xu X. , 2006. "Purification, characterization and cDNA cloning of a novel lipopolysaccharide-binding lectin from the shrimp Penaeus monodon ". Dev. Comp. Immunol., Vol 30: 607- 617. http://dx.doi.org/10.1016/j.dci.2005.10.004
12. Luo T. , Zhang X. , Shao Z. , Xu X. , 2003. "PmAV, a novel gene involved in virus resistance of shrimp Penaeus monodon ". FEBS Lett., Vol 551: 53- 57. http://dx.doi.org/10.1016/S0014-5793(03)00891-3
13. Ma T. H. T. , Benzie J. A. H. , He J.-G. , Chan S.-M. , 2008. "PmLT, a C-type lectin specific to hepatopancreas is involved in the innate defense of the shrimp Penaeus monodon ". Journ. Invertebr. Pathol., Vol 99: 332- 341. http://dx.doi.org/10.1016/j.jip.2008.08.003
14. Ma T. H. T. , Tiu S. H. K. , He J.-G. , Chan S.-M. , 2007. "Molecular cloning of a C-type lectin (LvLT) from the shrimp Litopenaeus vannamei: early gene down-regulation after WSSV infection". Fish Shellfish Immunol., Vol 23: 430- 437. http://dx.doi.org/10.1016/j.fsi.2006.12.005
15. Savan R. , Endo M. , Sakai M. , 2004. "Characterization of a new C-type lectin from common carp Cyprinus carpio ". Mol. Immunol., Vol 41: 891- 899. http://dx.doi.org/10.1016/j.molimm.2004.04.026
16. Sierra C. , Lascurain R. , Pereyra A. , Guevara J. , Martínez G. , Agundis C. , Zenteno E. , Vázquez L. , 2005. "Participation of serum and membrane lections on the oxidative burst regulation in Macrobrachium rosenbergiihemocytes". Dev. Comp. Immunol., Vol 29: 113- 121. http://dx.doi.org/10.1016/j.dci.2004.06.008
17. Söderhäll K. , Cerenius L. , 1998. "Role of the prophenoloxidase-activating system in invertebrate immunity". Curr. Opin. Immunol., Vol 10: 23- 28. http://dx.doi.org/10.1016/S0952-7915(98)80026-5
18. Soria F. , Sierra C. , Bouquelet S. , Brassart C. , Agundis C. , Zenteno E. , Vàzquez L. , 2006. "The effect of sugars and free amino acids from the freshwater prawn Macrobrachium rosenbergiihemolymph on lectin activity and on oxidative burst". Comp. Biochem. Physiol. C, Vol 142: 212- 219.
19. Sun Y.-D. , Fu L.-D. , Jia Y.-P. , Du X.-J. , Wang Q. , Wang Y.-H. , Zhao X.-F. , Yu X.-Q. , Wang J.-X. , 2008. "A hepatopancreas-specific C-type lectin from the Chinese shrimp Fenneropenaeus chinensisexhibits antimicrobial activity". Mol. Immunol., Vol 45: 348- 361. http://dx.doi.org/10.1016/j.molimm.2007.06.355
20. Sun J. , Wang L. , Wang B. , Guo Z. , Liu M. , Jiang K. , Tao R. , Zhang G. , 2008. "Purification and characterization of a natural lectin from the plasma of the shrimp Fenneropenaeus chinensis ". Fish Shellfish Immunol., Vol 25: 290- 297. http://dx.doi.org/10.1016/j.fsi.2008.06.001
21. Tamura K. , Duflry J. , Nei M. , Kumar S. , 2007. "MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0". Mol. Biol. Evol., Vol 24: 1596- 1599. http://dx.doi.org/10.1093/molbev/msm092
22. Vàzquez L. , Maldonado G. , Agundis C. , Pérez A. , Cooper E. L. , Zenteno E. , 1997. "Participation of a sialic acid-specific lectin from freshwater prawn Macrobrachium rosenbergiihemocytes in the recognition of non-self cells". Journ. Exp. Zool., Vol 279: 265- 272. http://dx.doi.org/10.1002/(SICI)1097-010X(19971015)279:3<265::AID-JEZ7>3.0.CO;2-L
23. Wang H. , Song L. , Li C. , Zhao J. , Zhang H. , Ni D. , Xu W. , 2007. "Cloning and characterization of a novel C-type lectin from Zhikong scallop Chlamys farreri ". Mol. Immunol., Vol 44: 722- 731. http://dx.doi.org/10.1016/j.molimm.2006.04.015
24. Wang X.-W. , Xu W.-T. , Zhang X.-W. , Zhao X.-F. , Yu X.-Q. , Wang J.-X. , 2009a. "A C-type lectin is involved in the innate immune response of Chinese white shrimp". Fish Shellfish Immunol., Vol 27: 556- 562. http://dx.doi.org/10.1016/j.fsi.2009.07.011
25. Wang X.-W. , Zhang X.-W. , Xu W.-T. , Zhao X.-F. , Wang J.-X. , 2009b. "A novel C-type lectin (FcLec4) facilitates the clearance of Vibrio anguillarumin vivo in Chinese white shrimp". Dev. Comp. Immunol., Vol 33: 1039- 1047. http://dx.doi.org/10.1016/j.dci.2009.05.004
26. Yamaura K. , Takahashi K. G. , Suzuki T. , 2008. "Identification and tissue expression analysis of C-type lectin and galectin in the Pacific oyster, Crassostrea gigas ". Comp. Biochem. Physiol. B: Biochem. Mol. Biol., Vol 149: 168- 175. http://dx.doi.org/10.1016/j.cbpb.2007.09.004
27. Zelensky A. N. , Gready J. E. , 2005. "The C-type lectin-like domain superfamily". FEBS Journ., Vol 272: 6179- 6217. http://dx.doi.org/10.1111/j.1742-4658.2005.05031.x
28. Zhang D. , Jiang K.-J. , Zhang F.-Y. , Ma C.-Y. , Shi Y.-H. , Qiao Z.-G. , Ma L.-B. , 2011. "Isolation and characterization of a ferritin cDNA from the mud crab Scylla paramamosain ". Journ. Crustacean Biol., Vol 31: 345- 351. http://dx.doi.org/10.1651/10-3340.1
29. Zhang H. , Robison B. , Thorgaard G. H. , Ristow S. S. , 2000. "Cloning, mapping and genomic organization of a fish C-type lectin gene from homozygous clones of rainbow trout ( Oncorhynchus mykiss)". Biochim. Biophys. Acta Gene Structure and Expression, Vol 1494: 14- 22. http://dx.doi.org/10.1016/S0167-4781(00)00198-6
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/content/journals/10.1163/156854012x650269
2012-07-07
2016-12-02

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