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The aim of this paper is to evaluate the long-term development of a hard bottom amphipod assemblage over a period of 12 years. Amphipod data were collected in the artificial reef of Fregene, situated in the central Tyrrhenian Sea (Italy). Amphipod samplings were carried out by scuba diving in two different periods: 1991-1992 (1st period) and 2001-2003 (2nd period). The amphipod assemblage’s structure and species composition changed over the years, as the species collected on the reef in the second period differed from those collected in the first. In addition, within the same timeframe the assemblage shows an increase in both abundance and diversity, as a result of the reef’s increase in spatial heterogeneity.