Cookies Policy
X

This site uses cookies. By continuing to browse the site you are agreeing to our use of cookies.

I accept this policy

Find out more here

High cross-family transferability of microsatellite markers developed for the snow crab, Chionoecetes opilio (Fabricius, 1788)

No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
The full text of this article is not currently available.

Brill’s MyBook program is exclusively available on BrillOnline Books and Journals. Students and scholars affiliated with an institution that has purchased a Brill E-Book on the BrillOnline platform automatically have access to the MyBook option for the title(s) acquired by the Library. Brill MyBook is a print-on-demand paperback copy which is sold at a favorably uniform low price.

Access this article

+ Tax (if applicable)
Add to Favorites
You must be logged in to use this functionality

image of Crustaceana

Of the seven species in the genus Chionoecetes (family Oregoniidae), the snow crab Chionoecetes opilio (Fabricius, 1788) is one of the most important commercial crustacean species along with its relative, the red snow crab C. japonicus Rathbun, 1932. Despite restrictions allowing male-only capture and size limits, the capture of C. opilio is changing and management of its natural resources is required. Microsatellite markers for C. opilio that can be used in population and management studies were isolated using 454 pyrosequencing, a cost-effective and rapid method. Among 11 172 dinucleotide microsatellites, 64 loci containing more than nine repeats of either CA or AT were selected for primer synthesis, and 49 (76.6%) primer sets producing PCR products between 100-300 bp in length were selected as candidates. Following initial experiments with four individuals of C. opilio, 18 polymorphic loci and three monomorphic loci were selected as the final microsatellite markers for this study. In a cross-species transfer analysis of these markers using 11 crab species belonging to the families Oregoniidae, Cheiragonidae and Portunidae in the order Brachyura, two loci, Co10-nfrdi and Co36-nfrdi, were amplified by PCR from all of the tested species, while the rest of the loci were amplified in at least one of the tested species. Among the 11 tested species, Portunus trituberculatus (Miers, 1876) of the family Portunidae showed the highest transferability. The average percentage of amplification was higher in the same genus followed by in the same family rather than in different genera or families.

Affiliations: 1: 1Biotechnology Research Division, NFRDI, Busan 619-705, South Korea; 2: 2Fisheries Resources Research Divison, NFRDI, Busan 619-705, South Korea; 3: 3Department of Biological Science, Silla University, Busan 617-736, South Korea

Loading

Full text loading...

/content/journals/10.1163/15685403-00003237
Loading

Data & Media loading...

1. Barbara T. , Palma Silva C. , Paggi G. M. , Bered F. , Fay M. F. , Lexer C. , 2007. "Cross-species transfer of nuclear microsatellite makers: potential and limitations". Mol. Ecol., Vol 16: 3759- 3767. http://dx.doi.org/10.1111/j.1365-294X.2007.03439.x
2. Chun Y. Y. , Hong B. G. , Hwang K. S. , Cha H. K. , Lee S. I. , Hwang S. J. , 2008. "Maturation of reproductive organs and spawning of the snow crab Chionoecetes opiliofrom the East Sea of Korea". J. Korean Fish. Soc., Vol 41: 119- 124.
3. Costello M. J. , Emblow C. S. , White R. (eds.), 2001. European register of marine species: a check-list of the marine species in Europe and a bibliography of guides to their identification. Collection Patrimoines Naturels, 284-292.
4. Danner S. , 2007. Snow and tanner crabs. Seafood report. Seafood Watch, West Coast Region. Monterey Bay Aquarium, Monterey, 41 pp.
5. Greenley A. P. , Muguia-Vega A. , Saenz-Arroyo A. , Micheli F. , 2012. "New tetranucleotide microsatellite loci in pink abalone ( Haliotis corrugata) isolated via 454 pyrosequencing". Conservation Genetics Resources, Vol 4: 265- 268. http://dx.doi.org/10.1007/s12686-011-9521-5
6. Hamilton M. , Fleischer E. L. , Di Fiore A. , Fleischer R. C. , 1999. "Universal linker and ligation procedures for construction of genomic DNA libraries enriched for microsatellites". Biotechniques, Vol 27: 500- 507.
7. Holmer O. , Black M. , Hoeh W. , 1994. "DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates". Molecular Marine Biology and Biotechnology, Vol 3: 294- 299.
8. Kang J.-H. , Park J.-Y. , Jo H.-S. , 2012. "Rapid development of microsatellite markers with 454 pyrosequencing in a vulnerable fish, the mottled skate, Raja pulchra ". Int. J. Mol. Sci., Vol 13: 7199- 7211. http://dx.doi.org/10.3390/ijms13067199
9. Kim W.-J. , Jung H. T. , Chun Y. Y. , Kang S. K. , Shin E.-H. , Kim Y.-O. , Nam B.-H. , Kong H. J. , Cha H. K. , 2012. "Genetic evidence for natural hybridization between red snow crab ( Chionoecetes japonicus) and snow crab ( Chionoecetes opilio) in Korea". Journal of Shellfish Research, Vol 31: 49- 56. http://dx.doi.org/10.2983/035.031.0106
10. KOSTAT (Statistics Korea), 2009. Statistical yearbook of agriculture and fisheries. ( Kostat, Seoul).
11. Luke S. J. , William E. D. , Paula C. , 1999. Biological field techniques for Chionoecetescrabs. (Published by University of Alaska Sea grant College Program, AK-SG-99-02).
12. Ma H. , Ma C. , Ma L. , Cui H. , 2010. "Novel polymorphic microsatellite markers in Scylla paramamosainand cross-species amplification in related crab species". Journal of Crustacean Biology, Vol 30: 441- 444. http://dx.doi.org/10.1651/09-3263.1
13. Merkouris S. E. , Seeb L. W. , 1998. "Low level of genetic diversity in highly exploited populations of Alaskan tanner crabs, Chionoecetes bairdi, and Alaskan snow crabs, C. opilio ". Fishery Bulletin, Vol 96: 525- 537.
14. Noor M. A. F. , Feder J. L. , 2006. "Speciation genetics: evolving approaches". Nature Reviews Genetics, Vol 7: 851- 861. http://dx.doi.org/10.1038/nrg1968
15. Orensanz J. M. , Armstrong J. , Armstrong D. , Hilborn R. , 1998. "Crustacean resources are vulnerable to serial depletion — the multifaced decline of crab and shrimp fisheries in the grater Gulf of Alaska". Rev. Fish Biol. Fish, Vol 8: 117- 176. http://dx.doi.org/10.1023/A:1008891412756
16. Puebla O. , Parent E. , Sevigny J. M. , 2003. "New microsatellite markers for the snow crab Chionoecetes opilio(Brachyura: Majidae)". Molecular Ecology Notes, Vol 3: 644- 646. http://dx.doi.org/10.1046/j.1471-8286.2003.00542.x
17. Puebla O. , Sevigny J. M. , Sainte-Marie B. , Brethes J.-C. , Burmeister A. D. , Dawe E. G. , Moriyasu M. , 2008. "Population genetic structure of the snow crab ( Chionoecetes opilio) at the northwest Atlantic scale". Can. J. Fish. Aquat. Sci., Vol 65: 425- 436. http://dx.doi.org/10.1139/f07-163
18. Squires H. J. , 1990. Decapod Crustacea of the Atlantic coast of Canada. Can. Bull. Fish. Aquat. Sci., 221 : 1- 532.
19. Sun Y. , Sun H. , Qian G. , Zhao Y. , Zhao Q. , Liu N. , 2009. "Isolation and characterization of microsatellite loci in the freshwater crab Sinopotamon yangtsekienseand cross-species amplification in related taxa (Decapoda: Brachyura)". Journal of Crustacean Biology, Vol 29: 413- 418. http://dx.doi.org/10.1651/08-3115.1
20. Urbani N. , Sevigny J. M. , Sainte-Marie B. , Zadworny Z. , Kuhnlein U. , 1998. "Identification of microsatellite makers in the snow crab Chionoecetes opilio ". Molecular Ecology, Vol 7: 357- 358. http://dx.doi.org/10.1046/j.1365-294X.1998.00306.x
21. Wang J. , Yu X. , Zhao K. , Zhang Y. , Tong J. , Peng Z. , 2012. "Microsatellite development for an endangered bream Megalobrama pellegrini(Teleostei, Cyprinidae) using 454 sequencing". Int. J. Mol. Sci., Vol 13: 3009- 3021. http://dx.doi.org/10.3390/ijms13033009
22. Whitham T. G. , Bailey J. K. , Schweitzer J. A. , Shuster S. M. , Bangert R. K. , Leroy C. J. , Lonsdorf E. V. , Allan G. J. , Difazio S. P. , Potts B. M. , Fischer D. G. , Gehring C. A. , Lindroth R. L. , Marks J. C. , Hart S. C. , Wimp G. M. , Wooley S. C. , 2006. "A framework for community and ecosystem genetics: from genes to ecosystems". Nature Reviews Genetics, Vol 7: 510- 523. http://dx.doi.org/10.1038/nrg1877
23. Zalapa J. E. , Cuevas H. , Zhu H. , Steffan S. , Senalik D. , Zeldin E. , McCown B. , Harbut R. , Simon P. , 2012. "Using next-generation sequencing approaches to isolate simple sequence repeat (SSR) loci in the plant sciences". Am. J. Bot., Vol 99: 193- 208. http://dx.doi.org/10.3732/ajb.1100394
24. Zane L. , Bargelloni L. , Patarnello T. , 2002. "Strategies for microsatellite isolation: a review". Molecular Ecology, Vol 11: 1- 16. http://dx.doi.org/10.1046/j.0962-1083.2001.01418.x
http://brill.metastore.ingenta.com/content/journals/10.1163/15685403-00003237
Loading

Article metrics loading...

/content/journals/10.1163/15685403-00003237
2013-01-01
2016-12-05

Sign-in

Can't access your account?
  • Tools

  • Add to Favorites
  • Printable version
  • Email this page
  • Subscribe to ToC alert
  • Get permissions
  • Recommend to your library

    You must fill out fields marked with: *

    Librarian details
    Your details
    Why are you recommending this title?
    Select reason:
     
    Crustaceana — Recommend this title to your library
  • Export citations
  • Key

  • Full access
  • Open Access
  • Partial/No accessInformation