Report of 14 novel microsatellites from the swimming crab Portunus trituberculatus (Miers, 1876) (Decapoda, Brachyura)
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The swimming crab, Portunus trituberculatus (Miers, 1876) is one of the most common edible marine crabs in East Asia. In this study, a multiple mixed probes hybridization method was used to isolate microsatellites to improve experimental efficiency and streamline the procedure. The mix of biotinylated probes included microsatellite GA, CA and CAT motifs. Fourteen novel polymorphic microsatellite loci were isolated and characterized from a wild population of P. trituberculatus. The number of alleles varied between three and nine, and the observed and expected heterozygosity at population level ranged from 0.542 to 1.000 and 0.616 to 0.851, respectively. Two loci, Ptr1 and Ptr3, significantly deviated from the Hardy-Weinberg equilibrium (P<0.01). These informative microsatellite markers could be useful for future population genetic analyses and genome mapping studies in this species.
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