Cookies Policy

This site uses cookies. By continuing to browse the site you are agreeing to our use of cookies.

I accept this policy

Find out more here

Effect of dietary protein level and salinity on growth, survival, enzymatic activities and amino-acid composition of the white shrimp Litopenaeus vannamei (Boone, 1931) juveniles

No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
The full text of this article is not currently available.

Brill’s MyBook program is exclusively available on BrillOnline Books and Journals. Students and scholars affiliated with an institution that has purchased a Brill E-Book on the BrillOnline platform automatically have access to the MyBook option for the title(s) acquired by the Library. Brill MyBook is a print-on-demand paperback copy which is sold at a favorably uniform low price.

Access this article

+ Tax (if applicable)
Add to Favorites
You must be logged in to use this functionality

image of Crustaceana

Litopenaeus vannamei (Boone, 1931) postlarvae with average initial body weight of 0.089 g were reared in 75-litre PVC tanks for 40 days at salinities of 30 and 60 g l−1. The shrimps were fed compound feed containing protein levels of 35, 40, 45 and 50%, respectively. Salinity had a remarkable effect on growth and survival of L. vannamei juveniles. Higher survival rate and lower growth were observed at 60 g l−1 salinity. Dietary protein level affected the survival and growth of juveniles at both salinities, increased with dietary protein levels in the range of 35 to 45%, but decreased slightly with 50% dietary protein. Broken line analysis showed that the estimated optimal dietary protein levels at salinities of 30 and 60 g l−1 were 45.93 and 46.74%, respectively. Higher salinity resulted in an increased moisture content, ash and crude protein content in the shrimp muscle tissue. The amino acid contents in the shrimp muscle tissue were generally higher at 60 g l−1 salinity and increased dietary protein level led to higher protein content, except with 50% dietary protein. At salinity 60 g l−1, the soluble protein content and activities of glutamic oxalacetic transferase (GOT) and glutamic pyruvic transaminase (GPT) in shrimp muscle tissue were higher, while catalase (CAT) activities were lower. Farming of L. vannamei at a marginal culture salinity (60 g l−1) is feasible though the shrimps were likely exposed to stressful conditions. Reduced growth rate at higher salinity may be attributed to the higher total ammonium (TAN) concentration in the culture medium and extra energy consumption for osmoregulation at hypersaline conditions.

Affiliations: 1: Tianjin Key Laboratory of Marine Resources and Chemistry, Tianjin University of Science and Technology, Tianjin 300457, P. R. China


Full text loading...


Data & Media loading...

1. Bao H. Y. , Shao P. , Zhang Q. , Liu B. Z. , Cao S. X. , Tang G. G. , 2012. "Ecological shrimp farming in salt ponds in northern China". Hebei Fisheries, Vol 225( 9): 13- 15. [In Chinese.]
2. Bradford M. M. , 1976. "A rapid and sensitive method for the quantization of microgram quantities of protein utilizing the principle of protein-dye binding". Anal. Biochem., Vol 72: 248- 254. [Crossref]
3. Bückle L. F. , Baron B. , Hermandez M. , 2006. "Osmoregulatory capacity of the shrimp Litopenaeus vannameiat different temperatures and salinities, and optimal culture environment". Rev. Biol. Trop., Vol 54( 3): 745- 753. [Crossref]
4. Castille F. L. , Lawrence A. L. , 1981. "The effect of salinity on the osmotic, sodium and chloride concentrations in the hemolymph of euryhaline shrimp of the genus Penaeus ". Comp. Biochem. Physiol., Vol 68A: 75- 80. [Crossref]
5. Cho C. Y. , Hynes J. D. , Wood K. R. , Toshida H. K. , 1994. "Development of high-nutrient-dense, low-pollution diets and prediction of aquaculture wastes using biological approaches". Aquaculture, Vol 124: 293- 305. [Crossref]
6. GB/T12763.4, 2007. National Standard of People’s Republic of China: specification for oceanographic survey. Part 4: survey of chemical parameters in seawater: 70-75.
7. Intanai I. , Taylor E. W. , Whiteley N. M. , 2009. "Effects of salinity on rates of protein synthesis and oxygen uptake in the post-larvae and juveniles of the tropical prawn Macrobrachium rosenbergii(de Man)". Comp. Biochem. Physiol., Vol 152A: 372- 378. [Crossref]
8. Jiang D. H. , Lawrence A. L. , Neil W. H. , Gong H. , 2000. "Effects of temperature and salinity on nitrogenous excretion by Litopenaeus vannameijuveniles". J. Exp. Mar. Biol. Ecol., Vol 253: 193- 209. [Crossref]
9. Kanazawa A. , 1989. "Protein requirements of penaeid shrimp. Advance in Tropical Aquaculture, Tahiti, Feb 20-March 4, 1989." AOUACOP IFREMER Actes de Colloque, Vol 9: 261- 270.
10. Li E. C. , Chen L. Q. , Zeng C. , Yu N. , Xiong Z. Q. , Chen X. F. , Qin I. G. , 2008. "Comparison of digestive and antioxidant enzymes activities, haemolymphy oxyhemocyanin contents and hepatopancrease histology of white shrimp, Litopenaeus vannameiat various salinities". Aquaculture, Vol 274: 80- 86. [Crossref]
11. Li E. C. , Chen L. Q. , Zeng C. , Xiong Z. Q. , Lin C. , Peng S. M. , Liu L. H. , 2009. "Protein accumulation, amino acid profile and amino transferase activities of the white shrimp, Litopenaeus vannameiat different salinities". Acta Hydrobiologia Sinica, Vol 33( 3): 532- 535. [In Chinese with English abstract.] [Crossref]
12. Peréz-Velázquez M. , González-Félix M. L. , Jaimes-Bustamente F. , Martínez-Córdova L. R. , Trujillo-Villalba D. A. , 2007. "Investigation of the effects of salinity and dietary protein level on growth and survival of pacific white shrimp, Litopenaeus vannamei ". J. World Aquacult. Soc., Vol 38( 4): 475- 485. [Crossref]
13. Stern S. , Daniels H. , Letellier E. , 1990. Tolerance of postlarvae and juvenile Penaeus vannameito low salinity. In: Abstracts of World Aquaculture 90, Halifax, Nova Scotia, Canada, 10-14 June 1990.
14. Velasco M. , Lawrence A. L. , Castille F. L. , Obaldoo L. G. , 2000. Dietary protein requirement for Litopenaeus vannamei. In: L. E. Cruz-Suarez, D. Ricque-Marie, M. Tapia-Salazar, M. A. Olvera-Novoa & R. Civera-Cerecedo(eds.). Avances en Nutricion Acuicola V. Memorias del V Simposium International de Nutricion Acuicola. Merida, Yucatan, Mexico. 19-22 November 2000: 181-192.

Article metrics loading...



Can't access your account?
  • Tools

  • Add to Favorites
  • Printable version
  • Email this page
  • Subscribe to ToC alert
  • Get permissions
  • Recommend to your library

    You must fill out fields marked with: *

    Librarian details
    Your details
    Why are you recommending this title?
    Select reason:
    Crustaceana — Recommend this title to your library
  • Export citations
  • Key

  • Full access
  • Open Access
  • Partial/No accessInformation