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Molecular cloning, characterization, and immune response against white spot syndrome virus and Taura syndrome virus infections of peroxiredoxin in Litopenaeus vannameiand its antioxidant activity

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Peroxiredoxin (Prx) is an important peroxidase that can protect organisms against various oxidative stresses. In this study, a member of Prx family, designated LvPrx, was cloned from Litopenaeus vannamei. Sequence and phylogenetic analyses indicated that LvPrx belongs to the 2-Cys Prx (Prx IV) isoform. The recombinant LvPrx protein was constructed and expressed in Escherichia coli, and the purified LvPrx proteins were shown to reduce H 2O 2in vitro in the presence of dithiothreitol, indicating that LvPrx is a functional peroxiredoxin. Using qRT-PCR, the mRNA expression levels of LvPrx were determined in the haemocytes of L. vannameiat different stages after being challenged with WSSV and TSV at different doses. The results showed that the expression levels of LvPrx were significantly up-regulated ( P < 0 . 05 ) during 4-24 h after both WSSV and TSV challenge, suggesting that LvPrx may participate in the shrimp’s immune response to viral infection.

Affiliations: 1: Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fisheries Sciences, Nanning 530021, P. R. China


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