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The full-length cDNA cloning and expression profiles of 14-3-3genes from the mud crab Scylla paramamosainEstampador, 1949

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Two full-length cDNA sequences of 14-3-3genes were isolated from the mud crab, Scylla paramamosainEstampador, 1949, using RT-PCR and RACE (rapid-amplification of cDNA ends). Sequence analysis indicates that both 14-3-3genes contain an open reading frame of 744 bp with a deduced 247-amino-acid protein. The gene and protein sequences of Sp 14-3-3genes show 96 and 97% identity, respectively, and both Sp 14-3-3cluster together with other animal 14-3-3 proteins in phylogenetic tree analysis. Tissue specific expression analysis reveals that both Sp 14-3-3genes are ubiquitously expressed, however, their expression patterns are different from each other. Expressions of both Sp 14-3-3genes are sensitive to salinity decrease, even a 5 ppt drop from 30 ppt to 25 ppt, indicating that Sp 14-3-3genes are involved in osmoregulation. Our observations may contribute to a better understanding of the molecular and functional evolution of the 14-3-3family in both crustaceans and (in)vertebrates as a whole.

Affiliations: 1: College of Animal Science, Zhejiang University, Hangzhou 310058, P. R. China

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/content/journals/10.1163/15685403-00003480
2015-11-04
2017-11-20

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