Cookies Policy
X

This site uses cookies. By continuing to browse the site you are agreeing to our use of cookies.

I accept this policy

Find out more here

Problems with the use of liposome- and retrovirus-mediated gene transfer methods in the primary lymphoid cells of the Oka organs of the greasyback shrimp, Metapenaeus ensis (De Haan, 1844)

No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
The full text of this article is not currently available.

Brill’s MyBook program is exclusively available on BrillOnline Books and Journals. Students and scholars affiliated with an institution that has purchased a Brill E-Book on the BrillOnline platform automatically have access to the MyBook option for the title(s) acquired by the Library. Brill MyBook is a print-on-demand paperback copy which is sold at a favorably uniform low price.

Access this article

+ Tax (if applicable)
Add to Favorites
You must be logged in to use this functionality

image of Crustaceana

In this study, both liposome- and retrovirus-mediated gene transfer methods were examined for their potential to transfer and express two retroviral vectors containing the mouse c-Myc or the green fluorescent protein (GFP) gene into the primary lymphoid cell cultures (OKA) derived from “Oka” organs (= organs of the lymphoid system) of the greasyback shrimp Metapenaeus ensis (De Haan, 1844). It was found that the c-Myc gene could be delivered into OKA cells by the liposome-mediated method, but the introduced c-Myc gene could not be effectively transcribed into mRNA. In contrast, the pantropic retrovirus-mediated method failed to introduce the c-Myc gene into OKA cells, and GFP was not detected in the transformed cells, either. This work inferred two problems for the use of the two above-mentioned gene transfer methods in the non-dividing OKA cells: (1) the viral promoter of long terminal repeats (LTRs) had low activity in shrimp cells; (2) the pantropic retrovirus-mediated gene transfer system had a low tropism to shrimp lymphoid cells.

Affiliations: 1: 1Key Laboratory of Marine Genetics and Breeding, Ministry of Education, Qingdao 266003, P. R. China

Loading

Full text loading...

/content/journals/10.1163/15685403-00003498
Loading

Data & Media loading...

1. Chen S. N. , Chi S. C. , Kou G. H. , Liao I. C. , 1986. "Cell culture from tissues of grass prawn, Penaeus monodon ". Fish Pathology, Vol 21: 161-166. [Crossref]
2. Chen S. N. , Wang C. S. , 1999. "Establishment of cell culture systems from penaeid shrimp and their susceptibility to white spot disease and yellow head viruses". Methods in Cell Science, Vol 21: 199-206. [Crossref]
3. Claydon K. , Katrina G. R. , Owens L. , 2010. "Attempts at producing a hybridized Penaeus mondon cell line by cellular fusion". Fish & Shellfish Immunology, Vol 29: 539-543. [Crossref]
4. Claydon K. , Owens L. , 2008. "Attempts at immortalization of crustacean primary cell cultures using human cancer genes". In Vitro Cellular & Developmental Biology: Animal, Vol 44: 451-457. [Crossref]
5. Crane M. S. J. , 1999. "Mutagensis and cell transformation in cell culture". Methods in Cell Science, Vol 21: 245-253. [Crossref]
6. De Filippis L. , Ferrari D. , Nodari L. R. , Amati B. , Snyder E. , Vescovi A. L. , 2008. "Immortalization of human neural stem cells with the c-Myc mutant T58A". PLoS ONE, Vol 3: e3310.
7. Dean D. A. , 1997. "Import of plasmid DNA into the nucleus is sequence specific". Experimental Cell Research, Vol 230: 293-302. [Crossref]
8. Dean D. A. , Strong D. D. , Zimmer W. E. , 2005. "Nuclear entry of nonviral vectors". Gene Therapy, Vol 12: 881-890. [Crossref]
9. Dwaine A. B. , Ellender R. D. , Middlebrooks B. L. , 1999. "Cell cycle components and their potential impact on the development of continuous in vitro penaeid cell replication". Methods in Cell Science, Vol 21: 255-261. [Crossref]
10. Fan T. J. , Wang X. F. , Shi Z. P. , Jin L. Y. , Wang M. , Lang G. H. , Tong S. L. , 2001. "Co-inducing effect of basic fibroblast growth factor and in sulin-like growth factor II on cultured lymphocytes from Penaeus chinensis ". Acta Oceanologica Sinica, Vol 23: 141-145.
11. Han Q. , Li P. T. , Lu X. B. , Guo Z. J. , Guo H. R. , 2013. "Improved primary cell culture and subculture of lymphoid organs of the greasyback shrimp Metapenaeus ensis ". Aquaculture, Vol 410: 101-113. [Crossref]
12. Hsu Y. L. , Yang Y. H. , Chen Y. C. , Tung M. C. , Wu J. L. , Engelking M. H. , Leong J. C. , 1995. "Development of an in vitro subculture system for the Oka organ (lymphoid tissue) of Penaeus monodon ". Aquaculture, Vol 136: 43-55. [Crossref]
13. Hu G. B. , Wang D. , Chen J. X. , 2010. "Retroviral delivery of Simian Virus 40 Large T antigen gene into primary cultured ovary cells of the penaeid shrimp, Penaeus chinensis: indirect evidence of retroviral integration". Journal of the World Aquaculture Society, Vol 41: 137-143. [Crossref]
14. Hu G. B. , Wang D. , Wang C. H. , Yang K. F. , 2008. "A novel immortalization vector for the establishment of penaeid shrimp cell lines". In Vitro Cellular & Developmental Biology: Animal, Vol 44: 51-56. [Crossref]
15. Jayesh P. , Seena J. , Singh I. B. , 2012. "Establishment of shrimp cell lines: perception and orientation". Indian Journal of Virology, Vol 23: 244-251. [Crossref]
16. Jiang Y. S. , Zhan W. B. , Wang S. B. , Xing J. , 2006. "Development of primary shrimp hemocyte cultures of Penaeus chinensis to study white spot syndrome virus (WSSV) infection". Aquaculture, Vol 53: 114-119. [Crossref]
17. Kasornchandra J. , Khongpradit R. , Ekpanithanpong U. , Boonyaratpalin S. , 1999. "Progress in the development of shrimp cell cultures in Thailand". Methods in Cell Science, Vol 21: 231-235. [Crossref]
18. Luedeman R. A. , Lightner D. V. , 1992. "Development of an in vitro primary cell culture system from the penaeid shrimp, Penaeus stylirostris and Penaeus vannamei ". Aquaculture, Vol 101: 205-211. [Crossref]
19. Maeda M. , Mizuki E. , Itami T. , Ohba M. , 2003. "Ovarian primary tissue culture of the kuruma shrimp Marsupenaeus japonicus ". In Vitro Cellular & Developmental Biology: Animal, Vol 31: 208-212. [Crossref]
20. May T. , Hauser H. , Wirth D. , 2004. "Transcriptional control of SV40 T-antigen expression allows a complete reversion of immortalization". Nucleic Acids Research, Vol 32: 5529-5538. [Crossref]
21. Mulford A. L. , Austin B. , 1998. "Development of primary cell cultures from Nephrops norvegicus ". Methods in Cell Science, Vol 19: 269-275. [Crossref]
22. Nadala E. C. , Lu Y. , Loh P. C. , 1993. "Primary culture of lymphoid, nerve and ovary cells from Penaeus stylirostris and Penaeus vannamei ". In Vitro Cellular & Developmental Biology: Animal, Vol 29: 620-622. [Crossref]
23. Shike H. , Shimizu C. , Klimpel K. S. , Burns J. C. , 2000. "Expression of foreign genes in primary cultured cells of the blue shrimp Penaeus stylirostris ". Marine Biology, Vol 137: 605-611. [Crossref]
24. Spann K. M. , Lester R. J. G. , 1997. "Viral diseases of penaeid shrimp with particular reference to four viruses recently found in shrimp from Queensland". World Journal of Microbiology and Biotechnology, Vol 13: 419-426. [Crossref]
25. Takahashi K. , Yamanaka S. , 2006. "Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors". Cell, Vol 126: 663-676. [Crossref]
26. Tapay L. M. , Lu Y. , Brock J. A. , Nadala E. C. B. , Loh P. C. , 1995. "Transformation of primary cultures of shrimp (Penaeus stylirostris) lymphoid (Oka) organ with simian virus-40 (T) antigen". Experimental Biology and Medicine, Vol 209: 73-78. [Crossref]
27. Tong S. L. , Miao H. Z. , 1996. "Attempts to initiate cell cultures from Penaeus chinensis tissues". Aquaculture, Vol 147: 151-157. [Crossref]
28. Toullec J. Y. , Crozat Y. , Patrois J. , Porcheron P. , 1996. "Development of primary cell cultures from the penaeid shrimps Penaeus vannamei and P. indicus ". Journal of Crustacean Biology, Vol 17: 643-649. [Crossref]
29. Xie S. , Sun L. , Liu F. , Dong B. , 2009. "Molecular characterization and mRNA transcript profile of vitellogenin in Chinese shrimp, Fenneropenaeus chinensis ". Molecular Biology Reports, Vol 36: 389-397. [Crossref]
30. Young J. L. , Benoit J. N. , Dean D. A. , 2003. "Effect of a DNA nuclear targeting sequence on gene transfer and expression of plasmids in the intact vasculature". Gene Therapy, Vol 10: 1465-1470. [Crossref]
31. Zhan W. B. , Wang Y. H. , Fryer J. L. , Yu K. K. , Fukuda H. , Meng Q. X. , 1998. "White spot syndrome virus infection of cultured shrimp in China". Journal of Aquatic Animal Health, Vol 10: 405-410. [Crossref]
32. Zhang X. J. , Yu L. M. , Li F. H. , Xiang J. H. , 2006. "Transfection and expression of the EGFP gene in the primarily cultured cells from Chinese shrimp Fenneropenaeus chinensis ". China Biotechnology, Vol 26: 38-43.
http://brill.metastore.ingenta.com/content/journals/10.1163/15685403-00003498
Loading

Article metrics loading...

/content/journals/10.1163/15685403-00003498
2015-12-03
2017-11-18

Sign-in

Can't access your account?
  • Tools

  • Add to Favorites
  • Printable version
  • Email this page
  • Subscribe to ToC alert
  • Get permissions
  • Recommend to your library

    You must fill out fields marked with: *

    Librarian details
    Your details
    Why are you recommending this title?
    Select reason:
     
    Crustaceana — Recommend this title to your library
  • Export citations
  • Key

  • Full access
  • Open Access
  • Partial/No accessInformation