Cookies Policy
X

This site uses cookies. By continuing to browse the site you are agreeing to our use of cookies.

I accept this policy

Find out more here

A new heat shock protein 70 gene (HSC70) and its expression profiles in response to cadmium stress and after different post-moulting times in Exopalaemon carinicauda (Holthuis, 1950) (Decapoda, Palaemonidae)

No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
The full text of this article is not currently available.

Brill’s MyBook program is exclusively available on BrillOnline Books and Journals. Students and scholars affiliated with an institution that has purchased a Brill E-Book on the BrillOnline platform automatically have access to the MyBook option for the title(s) acquired by the Library. Brill MyBook is a print-on-demand paperback copy which is sold at a favorably uniform low price.

Access this article

+ Tax (if applicable)
Add to Favorites
You must be logged in to use this functionality

image of Crustaceana

In this study, the full-length cDNA sequence (GenBank accession number AGF80339.1) encoding a novel heat shock protein HSP70 family member (Heat shock cognate 70, EcHSC70) was cloned from the ridgetail white prawn, Exopalaemon carinicauda (Holthuis, 1950) [currently also as: Palaemon carinicauda Holthuis, 1950]. EcHSC70 full-length cDNA consists of 2452 bp, containing an open reading frame (ORF) of 1935 bp, and it encodes a 650-amino-acid protein with a theoretical size of about 71 kDa and a predicted isoelectric point of 5.32. Phylogenetic analysis showed that EcHSC70 can be categorized together with the known HSP70 family members reported in other crustaceans. Tissue-expression analysis revealed that EcHSC70 was constitutively expressed in all of the tested tissues, with a significantly increased expression in the gill post-moulting. Moreover, the relative mRNA level of EcHSC70 tended to increase in the early stages of post-moulting (from 0 to 5 min), suggesting that EcHSC70 might take part in the recovery of E. carinicauda after moulting. In addition, under different levels of cadmium stress, EcHSC70 tended to be significantly expressed only after 24 h of cadmium exposure, and was more inducible by low concentrations of cadmium, as opposed to high concentrations.

Affiliations: 1: 1Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology, Lianyungang 222005, Jiangsu, P.R. China; 2: 4Zhejiang Institute of Freshwater Fisheries, Huzhou 313001, Zhejiang, P.R. China

Loading

Full text loading...

/content/journals/10.1163/15685403-00003520
Loading

Data & Media loading...

1. Boutet I. , Tanguy A. , Rousseau S. , Auffret M. , Moraga D. , 2003. "Molecular identification and expression of heat shock cognate 70 (hsc70) and heat shock protein 70 (hsp70) genes in the Pacific oyster Crassostrea gigas ". Cell Stress & Chaperones, Vol 8: 76-85. http://dx.doi.org/10.1379/1466-1268(2003)8<76:MIAEOH>2.0.CO;2
2. Bukau B. , Deuerling E. , Pfund C. , Craig E. A. , 2000. "Getting newly synthesized proteins into shape". Cell, Vol 101: 119-122. http://dx.doi.org/10.1016/S0092-8674(00)80806-5
3. Bukau B. , Horwich A. L. , 1998. "The Hsp70 and Hsp60 chaperone machines". Cell, Vol 92: 351-366. http://dx.doi.org/10.1016/S0092-8674(00)80928-9
4. Carlos A. G. , Daniela B. , Selene Z. , 2002. "Heat shock cognate protein 70 is involved in rotavirus cell entry". Journal of Virology, Vol 76: 4096-4102. http://dx.doi.org/10.1128/JVI.76.8.4096-4102.2002
5. Cesar J. R. O. , Yang J. , 2007. "Expression patterns of ubiquitin, heat shock protein 70, α-actin and β-actin over the molt cycle in the abdominal muscle of marine shrimp Litopenaeus vannamei ". Molecular Reproduction and Development, Vol 74: 554-559. http://dx.doi.org/10.1002/mrd.20605
6. Chuang K. H. , Ho S. H. , Song Y. L. , 2007. "Cloning and expression analysis of heat shock cognate 70 gene promoter in tiger shrimp (Penaeus monodon)". Gene, Vol 405: 10-18. http://dx.doi.org/10.1016/j.gene.2007.08.016
7. Clavero-Salas A. , Sotelo-Mundo R. R. , Gollas-Galvan N. , Hernandez-Lopez J. , Peregrino-Uriarte A. B. , Muhlia-Almazan A. , Yepiz-Plascencia G. , 2007. "Transcriptome analysis of gills from the white shrimp Litopenaeus vannamei infected with white spot syndrome virus". Fish Shellfish Immunology, Vol 23: 459-472. http://dx.doi.org/10.1016/j.fsi.2007.01.010
8. Dean R. J. , Shimmield T. M. , Black K. D. , 2007. "Copper, zinc and cadmium in marine cage fish farm sediments: an extensive survey". Environmental Pollution, Vol 145: 84-95. http://dx.doi.org/10.1016/j.envpol.2006.03.050
9. Feder M. E. , Hofmann G. E. , 1999. "Heat-shock proteins, molecular chaperones, and the stress response: evolutionary and ecological physiology". Annual Review of Physiology, Vol 61: 243-282. http://dx.doi.org/10.1146/annurev.physiol.61.1.243
10. Georgopoulos C. , Welch W. J. , 1993. "Role of the major heat shock proteins as molecular chaperones". Annual Review of Cell Biology, Vol 9: 601-634. http://dx.doi.org/10.1146/annurev.cb.09.110193.003125
11. Gilbert L. I. , Granger N. A. , Roe R. M. , 2000. "The juvenile hormones: historical facts and speculations on future research directions". Insect Biochemistry and Molecular Biology, Vol 30: 617-644. http://dx.doi.org/10.1016/S0965-1748(00)00034-5
12. Gunther E. , Walter L. , 1994. "Genetic aspects of the hsp70 multi-family in vertebrates". Experientia, Vol 50: 987-1001. http://dx.doi.org/10.1007/BF01923453
13. Hartl F. U. , 1996. "Molecular chaperones in cellular protein folding". Nature, Vol 381: 571-579. http://dx.doi.org/10.1038/381571a0
14. Holthuis L. B. , 1950. The Decapoda of the Siboga Expedition. Part X. The Palaemonidae collected by the Siboga and Snellius expeditions with remarks on other species. I. Subfamily Palaemoninae. Siboga Expeditie Monographie, 39a9 : 1-268.
15. Holthuis L. B. , 1980. FAO species catalogue. Shrimps and prawns of the world: an annotated catalogue of species of interest to fisheries. FAO Fisheries Synopsis, 125 : 1-271. (FAO, Rome).
16. Huberman A. , 2000. "Shrimp endocrinology: a review". Aquaculture, Vol 191: 191-208. http://dx.doi.org/10.1016/S0044-8486(00)00428-2
17. Jiang X. , Guan X. , Yao L. , Zhang H. , Jin X. , Han Y. , 2015. "Effects of single and joint subacute exposure of copper and cadmium on heat shock proteins in common carp (Cyprinus carpio)". Biological Trace Elements Research, Vol 169: 374-381. http://dx.doi.org/10.1007/s12011-015-0402-8
18. Jiao C. , Wang Z. , Li F. , Zhang C. , Xiang J. , 2004. "Cloning, sequencing and expression analysis of cDNA encoding a constitutive heat shock protein 70 (HSC70) in Fenneropenaeus chinensis ". Chinese Science Bulletin, Vol 49: 2385-2393.
19. Karouna-Renier N. K. , Zehr J. P. , 2003. "Short-term exposures to chronically toxic copper concentrations induce HSP70 proteins in midge larvae (Chironomus tentans)". The Science of the Total Environment, Vol 312: 267-272. http://dx.doi.org/10.1016/S0048-9697(03)00254-7
20. Kregel K. C. , 2002. "Heat shock proteins: modifying factors in physiological stress responses and acquired thermotolerance". Journal of Applied Physiology, Vol 92: 2177-2186. http://dx.doi.org/10.1152/japplphysiol.01267.2001
21. Larkin M. A. , Blackshields G. , Brown N. P. , Chenna R. , Mcgettigan P. A. , Mcwilliam H. , Valentin F. , Wallace I. M. , Wilm A. , Lopez R. , Thompson J. D. , Gibson T. J. , Higgins D. G. , 2007. "Clustal W and Clustal X version 2.0". Bioinformatics, Vol 23: 2947-2948. http://dx.doi.org/10.1093/bioinformatics/btm404
22. Lewis S. , Donkin M. E. , Depledge M. H. , 2001. "Hsp70 expression in Enteromorpha intestinalis (Chlorophyta) exposed to environmental stressors". Aquatic Toxicology, Vol 51: 277-291. http://dx.doi.org/10.1016/S0166-445X(00)00119-3
23. Liang J. P. , Li J. , Liu P. , Li J. , Chen P. , 2012. "Research progress of biological characteristics and artificial breeding of ridgetail white prawn, Exopalaemon carinicauda ". Chinese Agricultural Science Bulletin, Vol 28: 109-116.
24. Lindquist S. , Craig E. A. , 1988. "The heat-shock proteins". Annual Review of Genetics, Vol 22: 631-677. http://dx.doi.org/10.1146/annurev.ge.22.120188.003215
25. Lo W. Y. , Liu K. F. , Liao I. C. , Song Y. L. , 2004. "Cloning and molecular characterization of heat shock cognate 70 from tiger shrimp (Penaeus monodon)". Cell Stress & Chaperones, Vol 9: 332-343. http://dx.doi.org/10.1379/CSC-47R.1
26. Luan W. , Li F. , Zhang J. , Wen R. , Li Y. , Xiang J. , 2010. "Identification of a novel inducible cytosolic Hsp70 gene in Chinese shrimp Fenneropenaeus chinensis and comparison of its expression with the cognate Hsc70 under different stresses". Cell Stress & Chaperones, Vol 15: 83-93. http://dx.doi.org/10.1007/s12192-009-0124-y
27. Moseley P. L. , 1997. "Heat shock proteins and heat adaptation of the whole organism". Journal of Applied Physiology, Vol 83: 1413-1417.
28. Multhoff G. , 2007. "Heat shock protein 70 (Hsp70): membrane location, export and immunological relevance". Methods, Vol 43: 229-237. http://dx.doi.org/10.1016/j.ymeth.2007.06.006
29. Parsell D. A. , Lindquist S. , 1993. "The function of heat-shock proteins in stress tolerance: degradation and reactivation of damaged proteins". Annual Review of Genetics, Vol 27: 437-496. http://dx.doi.org/10.1146/annurev.ge.27.120193.002253
30. Qian Z. , Liu X. , Wang L. , Wang X. , Li Y. , Xiang J. , Wang P. , 2012. "Gene expression profiles of four heat shock proteins in response to different acute stresses in shrimp, Litopenaeus vannamei ". Comparative Biochemistry and Physiology, Part C, Vol 156: 211-220.
31. Qian Z. , Mi X. , Wang X. , He S. , Liu Y. , Hou F. , Liu Q. , Liu X. , 2013. "cDNA cloning and expression analysis of myostatin/GDF11 in shrimp, Litopenaeus vannamei ". Comparative Biochemistry and Physiology, Part A, Vol 165: 30-39. http://dx.doi.org/10.1016/j.cbpa.2013.02.001
32. Ranford J. C. , Henderson B. , 2002. "Chaperonins in disease: mechanisms, models, and treatments". Journal of Clinical Pathology: Molecular Pathology, Vol 55: 209-213. http://dx.doi.org/10.1136/mp.55.4.209
33. Riddiford L. M. , Hiruma K. , Zhou X. , Nelson C. A. , 2003. "Insights into the molecular basis of the hormonal control of molting and metamorphosis from Manduca sexta and Drosophila melanogaster ". Insect Biochemistry and Molecular Biology, Vol 33: 1327-1338. http://dx.doi.org/10.1016/j.ibmb.2003.06.001
34. Schmittgen T. D. , Livak K. J. , 2008. "Analyzing real-time PCR data by the comparative CT method". Nature Protocols, Vol 3: 1101-1108. http://dx.doi.org/10.1038/nprot.2008.73
35. Templeton D. M. , Liu Y. , 2010. "Multiple roles of cadmium in cell death and survival". Chemico-Biological Interaction, Vol 188: 267-275. http://dx.doi.org/10.1016/j.cbi.2010.03.040
36. Webster S. G. , Keller R. , 1986. "Purification, characterization and amino acid composition of the putative moult-inhibiting hormone (MIH) of Carcinus maenas (Crustacea, Decapoda)". Journal of Comparative Physiology, Part B, Vol 156: 611-624. http://dx.doi.org/10.1007/BF00692737
37. Whiteley N. M. , El-Haj A. J. , 1997. "Regulation of muscle gene expression over the moult in Crustacea". Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, Vol 117: 323-331. http://dx.doi.org/10.1016/S0305-0491(97)00130-2
38. Witeska M. , Jezierska B. , Chaber J. , 1995. "The influence of cadmium on common carp embryos and larvae". Aquaculture, Vol 129: 129-132. http://dx.doi.org/10.1016/0044-8486(94)00235-G
39. Xu W. , Xie J. , Shi H. , Li C. , 2010. "Hematodinium infections in cultured ridgetail white prawns, Exopalaemon carinicauda, in eastern China". Aquaculture, Vol 300: 25-31. http://dx.doi.org/10.1016/j.aquaculture.2009.12.024
40. Zhang J. , Wang J. , Gui T. , Sun Z. , Xiang J. , 2014. "A copper-induced metallothionein gene from Exopalaemon carinicauda and its response to heavy metal ions". International Journal of Biological Macromolecules, Vol 70: 246-250. http://dx.doi.org/10.1016/j.ijbiomac.2014.06.020
41. Zheng W. W. , Yang D. T. , Wang J. X. , Song Q. S. , Gilbert L. I. , Zhao X. F. , 2010. "Hsc70 binds to ultraspiracle resulting in the upregulation of 20-hydroxyecdsone-responsive genes in Helicoverpa armigera ". Molecular and Cellular Endocrinology, Vol 315: 282-291. http://dx.doi.org/10.1016/j.mce.2009.10.018
http://brill.metastore.ingenta.com/content/journals/10.1163/15685403-00003520
Loading

Article metrics loading...

/content/journals/10.1163/15685403-00003520
2016-03-15
2018-08-18

Sign-in

Can't access your account?
  • Tools

  • Add to Favorites
  • Printable version
  • Email this page
  • Subscribe to ToC alert
  • Get permissions
  • Recommend to your library

    You must fill out fields marked with: *

    Librarian details
    Your details
    Why are you recommending this title?
    Select reason:
     
    Crustaceana — Recommend this title to your library
  • Export citations
  • Key

  • Full access
  • Open Access
  • Partial/No accessInformation