Cookies Policy
X

This site uses cookies. By continuing to browse the site you are agreeing to our use of cookies.

I accept this policy

Find out more here

Cryopreservation of Chinese mitten crab, Eriocheir sinensis H. Milne Edwards, 1853 (Decapoda, Brachyura), embryos by vitrification

No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
The full text of this article is not currently available.

Brill’s MyBook program is exclusively available on BrillOnline Books and Journals. Students and scholars affiliated with an institution that has purchased a Brill E-Book on the BrillOnline platform automatically have access to the MyBook option for the title(s) acquired by the Library. Brill MyBook is a print-on-demand paperback copy which is sold at a favorably uniform low price.

Access this article

+ Tax (if applicable)
Add to Favorites
You must be logged in to use this functionality

image of Crustaceana

Though cryopreservation of some invertebrate embryos was successful, the cryopreservation of crustacean embryos has not yet been reported. On the basis of previous study, a single vitrifying cocktail (Code A: 30% propanediol and 20% dimethyl formamide) was selected as a cryoprotectant from six kinds of vitrifying solutions to cryopreserve E. sinensis embryos. The cleavage stage, the gastrula, the pre-nauplius, and the first zoea stage embryos were serially acclimated in 25, 33, 50, 67 and 100% vitrifying solution A for 6 min. Embryos were acclimated for 1 min on the 20 cm liquid nitrogen (LN2) layer, and then plunged into liquid nitrogen. After storage for several minutes in LN2, straws containing frozen embryos were acclimated for 1 min on the 20 cm liquid nitrogen (LN2) layer, then quickly removed from LN2. For thawing, straws were immersed quickly for 1 min in a water bath at 37°C, then carefully washed with 0.25 mol/l sucrose and were then incubated with 15 ppt seawater in a Petri dish at 25°C. The survival rate in the vitrifying solution differed for embryos in different stages of development, and the survival rate of different stage embryos declined with the increase of acclimation time in code A vitrifying solution. The survival rate of pre-nauplius stage embryos did not significantly differ when the embryos were washed for 5, 10, 15, or 20 min with 0.25 mol/l sucrose after thawing. There was no survival after either cleavage stage embryos, or gastrula stage embryos were frozen. Eight pre-nauplius stage embryos survived and the survival rate was 9.3 ± 2.5%, but subsequently the embryos died at the fourth day. Seven first zoea stage embryos survived, and the survival rate was 11.3 ± 3.6%, one frozen-thawed embryo hatched at the seventh day. We conclude that cryopreservation of E. sinensis embryos by vitrification is feasible.

Affiliations: 1: East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of East China Sea & Oceanic Fishery Resources Exploitation and Utilization, MOA, Shanghai 200090, P. R. China

1Corresponding author; e-mail: pzhuang@ecsf.ac.cn
Loading

Full text loading...

/content/journals/10.1163/15685403-00003730
Loading

Data & Media loading...

1. Alfaro J., Komen J., Huisman E. A., 2001. "Cryoprotectant and hypersaline sensitivity of penaeid shrimp embryos and nauplius larvae". Aquaculture, Vol 195: 353-366. [Crossref]
2. Ali J., Shelton J. N., 1993. "Design of vitrification solutions for the cryopreservation of embryos". Journal of Reproduction and Fertility, Vol 99: 471-477. [Crossref]
3. Arakawa T., Carpenter J. F., Kita Y. A., Crowe J. H., 1990. "The basis for toxicity of certain cryoprotectants: a hypothesis". Cryobiology, Vol 27: 401-415. [Crossref]
4. Chao N. H., Liao I. C., 2001. "Cryopreservation of finfish and shellfish gametes and embryos". Aquaculture, Vol 197: 161-189. [Crossref]
5. Chao N. H., Lin T. T., Chen Y. J., Hsu H. W., Liao I. C., 1997. "Cryopreservation of late embryos and early larvae in the oyster and hard clam". Aquaculture, Vol 15: 531-544.
6. Chapelle S., 1986. "Aspects of phospholipid metabolism in crustaceans as related to changes in environmental temperatures and salinities". Comp. Biochem. Physiol., Vol 84B: 423-439.
7. Chen S. L., Tian Y. S., 2005. "Cryopresercation of flounder (Paralichthys olivaceus) embryos by vitrification". Theriogenology, Vol 63: 1207-1219. [Crossref]
8. Dobrinsky J. R., 2002. "Advancements in cryopreservation of domestic animal embryos". Theriogenology, Vol 57: 285-302. [Crossref]
9. Douchi O., Takakura H., Imai K., 1990. "Transfer of bovine embryos cryopreserved by vitrification". Journal of Animal Reproduction, Vol 36: 69-72. [Crossref]
10. Gwo J. C., 2000. "Cryopreservation of eggs and embryos from aquatic organisms". In: Tiersch T. R., Mazik P. M. (eds.), Cryopreservation in aquatic species: 211-229. (World Aquaculture Society, Baton Rouge, LA).
11. Hagedorn M., Kleinhans F. W., Artemov D., Pilatus U., 1988. "Characterization of a major permeability barrier in the zebrafish embryo". Biology of Reproduction, Vol 59: 1240-1250. [Crossref]
12. Hagedorn M., Kleinhans F. W., Wildt D. E., Rall W. F., 1977. "Chill sensitivity and cryoprotectant permeability of dechorionated zebrafish embryos, Brachydanio rerio". Cryobiology, Vol 34: 251-263. [Crossref]
13. Harrison K. E., 1990. "The role of nutrition in maturation, reproduction and embryonic development of decapods crustaceans: a review". J. Shellfish. Res., Vol 9: 1-28.
14. Hochi S., Fujimoto T., Braun J., Oguri N., 1994. "Pregnancies following transfer of equine embryos cryopreserved by vitrification". Theriogenology, Vol 42: 483-488. [Crossref]
15. Huang X. R., Zhuang P., Zhang L. Z., Feng G. P., Liu J. Y., Zhang T., Zhao F., 2013. "Effects of different vitrificant solutions on the embryos of the Chinese mitten crab Eriocheir sinensis (Decapoda, Brachyura)". Crustaceana, Vol 86: 1-15. [Crossref]
16. Huang X. R., Zhuang P., Zhang L. Z., Yao Z. F., Liu T., Liu J. Y., Feng G. P., 2011. "Effects of cryoprotectant toxicity on embryos of the Chinese mitten crab, Eriocheir sinensis (Decapoda, Brachyura)". Crustaceana, Vol 84: 281-291. [Crossref]
17. Musa M. A., Rana K. J., McAndrew J., 1995. "Effect of cryoprotectants on activity of selected enzymes in fish embryos". Cryobiology, Vol 32: 92-104. [Crossref]
18. Newton S. S., Subramoniam T., 1996. "Cryoprotectant toxicity in penaeid prawn embryos". Cryobiology, Vol 33: 172-177. [Crossref]
19. Olive P. J., Wang W. B., 1997. "Cryopreservation of Nereis virens (Polychaeta, Annelida) larvae: the mechanism of cryopreservation of a differentiated metazoan". Cryobiology, Vol 34: 284-294. [Crossref]
20. Pan H. Q., 2002. Ecological culture of Chinese mitten crab. (China Agriculture Science and Technology Press, Beijing). (In Chinese).
21. Rall W. F., Fahy G. M., 1985. "Ice-free cryopreservation of mouse embryos at −196°C by vitrification". Nature, Vol 313: 573-585. [Crossref]
22. Ravid T., Tietz A., Khayat M., Boehm E., Michelis R., Lubzens E., 1999. "Lipid accumulation in the ovaries of a marine shrimp Penaeus semisulcatus". J. Exp. Biol, Vol 202: 1819-1829.
23. Renard P., 1991. "Cooling and freezing tolerance in embryos of the Pacific oyster, Crassostrea gigas: methanol and sucrose effects". Aquaculture, Vol 92: 43-57. [Crossref]
24. Subramoniam T., Arun R., 1999. "Cryopreservation and aquaculture: a case study with penaeid shrimp larvae". Curr. Sci., Vol 76: 361-368.
25. Urbanyi B., Baranyai B., Magyary I., Dibbyed A., 1997. "Toxicity of methanol, DMSO and glycerol on carp (Cyprinus carpio) embryos in different developmental stages". Theriogenology, Vol 47: 408. [Crossref]
26. Vuthiphandchai V., Pengpun B., Nimrat S., 2005. "Effects of cryoptotectant toxicity and temperature sensitivity on the embryos of black tiger shrimp (Penaeus monodon)". Aquaculture, Vol 246: 275-284. [Crossref]
27. Wang W. B., Olive P. J., 2000. "Effects of cryopreservation on the ultrastructural anatomy of 3-segment Nereis virens larvae (Polychaeta)". Invertebrate Biology, Vol 119: 309-318. [Crossref]
28. Xu Q. Q., Wang H. Z., Zhang S. P., 2003. "The impact of overstocking of mitten crab, Eriocheir sinensis, on lacustrine zoobenthos community". Acta Hydrobiologica Sinica, Vol 27: 41-46. (In Chinese).
29. Zhang T., Rawson D. M., 1996. "Feasibility studies on vitrification of intact zebrafish (Brachydanio rerio) embryos". Cryobiology, Vol 33: 1-13. [Crossref]
30. Zhang T., Rawson D. M., Morris G. J., 1993. "Cryopreservation of pre-hatch embryos of zebrafish (Brachydanio rerio)". Aquat. Living Res., Vol 6: 145-153. [Crossref]
31. Zhang X. S., Zhao L., Hua T. C., Zhu H. Y., 1989. "A study on the cryopreservation of common carp (Cyprinus carpio) embryos". Cryoletters, Vol 10: 271-278.
32. Zhao N. G., 2000. "The industrial culture of Chinese mitten crab in China and the prospect of its development". Science of Fisheries Farm, Vol 6: 5-6.
http://brill.metastore.ingenta.com/content/journals/10.1163/15685403-00003730
Loading

Article metrics loading...

/content/journals/10.1163/15685403-00003730
2017-11-10
2018-09-22

Sign-in

Can't access your account?
  • Tools

  • Add to Favorites
  • Printable version
  • Email this page
  • Subscribe to ToC alert
  • Get permissions
  • Recommend to your library

    You must fill out fields marked with: *

    Librarian details
    Your details
    Why are you recommending this title?
    Select reason:
     
    Crustaceana — Recommend this title to your library
  • Export citations
  • Key

  • Full access
  • Open Access
  • Partial/No accessInformation