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Preliminary Study of the Isoenzymes of Ostracoda

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Esterases and acid phosphatases were studied of 6 species of ostracod genera Cyprinotus Br. and Notodromas Lillj. from Poland, using method of electrophoretic separation of isoenzymes on polyacrylamide gel (no zymograms could be obtained of the peroxidase and dehydrogenase isoenzymes). For separations a plate device was applied, which enabled to perform accurate comparisons of the pictures obtained. There were about 20 specimens of given species in a sample, thus zymograms of mentioned proteins "resultant" of studied population were examined. Dependence of the esterase and acid phosphatase zymograms on ontogenetic development, sex, species and morphological variation within the species was analysed. Esterases appear to be more "inert" than the acid phosphatases (they are markedly less strongly differentiated than acid phosphatases ; the conclusions on similarity between the species as determined by the former coincide with the conclusions from the morphological studies, otherwise than in the case of the latter). Usefulness of results obtained is discussed, and necessity of further studies is proved.

Affiliations: 1: Institute of Biology, University of Gdańsk, Gdańsk, and Institute of Plant Genetics, Poznań, Poland

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/content/journals/10.1163/156854078x00411
1978-01-01
2016-08-28

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