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DETECTION OF GENETIC VARIABILITY IN STYGOBITIC ISOPODS USING RAPD MARKERS

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Three species of the stygobitic isopod genus Stenasellus have been described from Italy: Stenasellus racovitzai, S. nuragicus, and S. assorgiai. Recent electrophoretic data suggest the presence of more than one species in the "racovitzai"-group. In order to clarify the degree of genetic variability and the evolutionary trends in the "racovitzai-group, we have analysed three natural populations currently known from Italy and Corsica, by the Random Amplified Polymorphic DNA (RAPD)" technique. RAPD has proven to be a rapid and valid method for the detection of intra and interspecific genetic polymorphism in Crustacea and to be suitable for the analysis of DNA of preserved samples. This technique was carried out to generate RAPD fingerprints from the populations of the five localities. The molecular data obtained by RAPD markers confirm previously obtained morphological and electrophoretic results.

Trois especes du genre d'Isopodes stygobie Stenasellus ont ete decrite d'Italie: Stenasellus racovitzai, S. nuragicus et S. assorgiai. De recentes donnees electrophoretiques suggerent la presence de plus d'une espece dans le groupe "racovitzai. Afin de clarifier le degre de variabilite genetique et la tendance evolutionnaire dans ce groupe, nous avons analyse trois populations naturelles couramment connues d'Italie et de Corse par la technique "Random Amplified Polymorphic DNA (RAPD)". Cette technique s'est revelee une methode rapide et valable de detection du polymorphisme genetique intra- et interspecifique chez les Crustaces, et appropriee a l'analyse de DNA des echantillons conserves. Cette technique a ete effectuee pour produire les empreintes RAPD des populations des cinq localites. Les donnees moleculaires obtenues par les marqueurs RAPD confirment les resultats morphologiques et electrophoretiques precedemment obtenus.

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/content/journals/10.1163/156854099503672
1999-10-01
2016-08-24

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