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Nematode specific gravity profiles and applications to flotation extraction and taxonomy

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For more content, see Nematologica.

A technique is described that refines the standard sugar flotation procedure used to isolate nematodes from their surroundings. By centrifuging nematodes in a number of increasing specific gravity solutions and plotting the fraction floating, the cumulative probability distribution of the population’s specific gravity is generated. By assuming normality, the population mean, μ, and standard deviation, σ, are found by a nonlinear least squares procedure. These density parameters along with their error covariance matrix may be used as a taxonomic physical character. A chi-squared test is derived for comparing populations. Mean and standard deviation pairs (μ, σ) were found for the specific gravities of the adult stage of the plant parasites Pratylenchus agilis (1.068, 0.017), P. scribneri (1.073, 0.028), P. penetrans (1.058, 0.008) and the bacterial-feeder Caenorhabditis elegans (1.091, 0.016).

La technique exposée affine le procédure standard par flottation au sucre utilisée pour séparer les nématodes de leur milieu. La centrifugation des nématodes dans une série de solutions de densités spécifiques et la mise en diagramme de la valeur de la fraction surnageante permettent de connaître le répartition de la probabilité cumulée de la densité spécifique de la population en cause. La normalité étant supposée, la moyenne de la population, μ, et la déviation standard, σ, sont calculées par la méthode des moindres carrés non linéaires. Ces paramètres relatifs à la densité ainsi que leur matrice de co-variance d’erreur peuvent être utilisés en taxinomie comme caractère physique. Un test chi2 en est dérivé pour comparer les populations entre elles. Des données en paires — moyenne (μ) et écart-type (σ) — ont été définies pour les densités des adultes des espèces phytoparasites Pratylenchus agilis (1,068; 0,017), P. scribneri (1,073; 0,028), P. penetrans (1,058; 0,008), ainsi que pour l’espèce bactérivore Caenorhabditis elegans (1,091; 0,016).


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