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Protein variation in tropical Meloidogyne spp. as shown by two-dimensional electrophoregram computed analysis

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For more content, see Nematologica.

Protein variation and phenetic relationships in four tropical root-knot nematode species were studied by two-dimensional gel electrophoresis (2-DGE). Total soluble protein extracts from white females of Meloidogyne incognita, M. javanica, M. arenaria and M. mayaguensis were separated by electrophoresis, and the two-dimensional (2-D) profiles were compared with a computed analysis system. A four species global comparison allowed detection of 4 and 13 specific proteins for M. arenaria and M. mayaguensis, respectively, among the 498 proteinic spots detected on the synthetic image (or 'master'). Such proteins were not detected for M. incognita and M. javanica. Nevertheless, species discriminative proteins and shared proteins have been identified in all pairwise comparisons between species. In addition, the similarity index and the genetic distances between the different isolates studied were calculated on the basis of homologous polymorphic spots. A dendrogram was constructed according to the UPGMA method. Significance of the results from phenetic study was assessed by bootstrap analysis. M. incognita, M. javanica and M. arenaria showed high similarity. M. mayaguensis was more distant. These results are in agreement with RAPD and RFLP studies.

La variabilité protéique et les relations phénétiques de quatre espèces tropicales de nématodes galligènes ont été étudiées par électrophorèse sur gel en deux dimensions (2-DGE). Les protéines solubles totales extraites de femelles blanches de Meloidogyne incognita, M. javanica, M. arenaria et M. mayaguensis ont été séparées par électrophorèse et les profils bidimensionels comparés à l'aide d'un système d'analyse informatisé. Une comparaison globale des quatre espèces a permis de détecter quatre protéines spécifiques pour M. arenaria et 13 pour M. mayaguensis parmi les 498 taches protéiques présentes sur l'image de référence (ou “master”). De telles protéines n'ont été détectées ni pour M. incognita ni pour M. javanica. Néanmoins, des protéines discriminantes et communes ont été identifiées lors de toutes les comparaisons d'espèces deux à deux. De plus, les indices de similarité et les distances génétiques entre les différents isolats étudiés ont été calculés à partir des taches polymorphes homologues. Les dendrogrammes ont été construits selon la méthode UPGMA. La signification des résultats phénétiques a été estimée par une analyse “bootstrap”. M. incognita, M. javanica et M. arenaria sont très proches. M. mayaguensis est plus distant. Ces résultats sont en accord avec ceux d'études en RAPD et RFLP.

Affiliations: 1: UMR INRA/ENSAR Biologie des Organismes et des Populations Appliquée à la Protection des Plantes, B. P. 35327, 35653 Le Rheu cedex, France


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