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Use of a monoclonal antibody in a field evaluation of the persistence and infectivity of Steinernema n. sp. D1 (Nematoda: Steinernematidae)

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For more content, see Nematologica.

At the end of a mushroom cropping house trial designed to determine the infectivity, to phorid flies, of the entomopathogenic nematode Steinernema D1 (isolate IOW45), the spent, nematode-infested substrate was deposited as a stack on the edge of a field. The introduced population of Steinernema D1 (IOW45) was monitored by bi-monthly sampling for eight months. The infectivity, to Galleria mellonella larvae, of nematodes extracted from cores of substrate taken from the stack, was determined. There was a decline (P < 0.01) in the numbers of nematodes extracted on each of the sample dates. After the first sample was taken, fewer (P < 0.001) nematodes were recovered from the top layer of the stack than from the bottom or middle layers. Nematode infectivity declined (P < 0.001) over the first two months in the field and then remained at a constant low level until month 8, when no G. mellonella were parasitised. After removal of the stack, subsequent soil samples taken from plots that had been underlying, or adjacent to it, were baited with G. mellonella larvae. Samples of the haemolymph taken from larvae that died in the soil were tested with a monoclonal antibody specific to the bacterial symbiont (Xenorhabdus sp.) of Steinernema D1 (IOW45). Using this method, Steinernema D1 (IOW45) was detectable in soil that had been underlying the compost stack for as long as 12 months after compost deposition.

Utilisation d'anticorps monoclonaux pour l'évaluation au champ de la persistanceet du pouvoir infestant de Steinernema n. sp. D1 (Nematoda: Steinernematidae) - A la fin d'un essai sur une culture de champignons sous abri, essai destiné à déterminer le pouvoir infestant du nématode entomopathogène Steinernema D1 (isolat IOW45) envers des Diptères Phoridae, le substrat utilisé, infesté de nématodes, a été rassemblé en une meule, au coin du champ. La population de Steinernema D1 (IOW45) a ensuite été évaluée par des prélèvements bimensuels pendant 8 mois. Le pouvoir infestant des nématodes envers des larves de Galleria mellonella a été déterminé en prélevant des carottes dans la meule de substrat. Il s'est révélé que le nombre de nématodes extraits diminue (P < 0,01) au cours des prélèvements successifs. Après le premier prélèvement, il est récupéré moins de nématodes (P < 0,001) au sommet de la meule qu'au milieu ou au bas de celle-ci. Au champ, le pouvoir infestant du nématode décroit (P < 0,001) pendant les 2 premiers mois et reste ensuite au même niveau jusqu'au huitième mois où aucun G. mellonella n'est plus parasité. Après enlèvement de la meule, des échantillons supplémentaires dans le sol situé sous la meule ou à proximité de celle-ci ont été pourvus d'appâts (larves de G. mellonella). Des échantillons d'hémolymphe provenant de larves mortes dans le sol ont été testés à l'aide d'un anticorps monoclonal spécifique de la bactérie symbiote (Xenorhabdus sp.) de Steinernema D1 (IOW45). En utilisant cette méthode, Steinernema D1 (IOW45) peut être détecté dans le sol situé sous la meule pendant une période d'au moins 2 mois après l'enlèvement du compost.

Affiliations: 1: Department of Entomological Sciences, Horticulture Research International, Wellesbourne, Warwick CV35 9EF,UK


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