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Molecular characterisation of Heterorhabditis indica isolates from India, Kenya, Indonesia and Cuba

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Isolates of Heterorhabditis were identified as H. indica using the following molecular diagnostic features: hybridisation to a H. indica specific satellite DNA probe; AluI and MboI restriction profiles of the rDNA ITS PCR product and the AluI profile of the rDNA IGS PCR product. The Kenyan isolates represent a distinct subgroup of H. indica. These isolates lacked one of the two HinfI restriction sites which are present in the rDNA ITS product of all the other isolates tested and they also differed from other H. indica isolates in their rDNA IGS HaeIII restriction profile. The Indian isolates are interfertile. The Kenyan isolates are interfertile but only one Kenyan isolate, Ki3, produced viable progeny when crossed with H. indica LN2. The four Indonesian isolates are interfertile, but only one Indonesian isolate (INA H1) produced viable hybrids when crossed with H. indica LN2. INA H1 was also interfertile with the Kenyan isolate Ki3.

Caractérisation moléculaire d'isolats d'Heterorhabditis indica provenant d'Inde, du Kenya, d'Indonésie et de Cuba - Des isolats d'Heterorhabditis ont été identifiés comme H. indica par l'utilisation des techniques de caractérisation moléculaire suivantes: hybridation avec une sonde spécifique du DNA satellite de H. indica, produits des profils de restriction par PCR de l'ITS du rDNA par AluI et MboI et produit de PCR de l'IGS du rDNA par AluI. Les isolats keniyans constituent un sous-groupe distinct d'H. indica. Un des deux sites de restriction de HinfI, présent dans les produits de l'ITS du rDNA de tous les autres isolats étudiés, est absent dans ces isolats qui différaient également dans leurs profils de restriction de l'IGS du rDNA par HaeIII. Les isolats d'Inde sont interfertiles. Les isolats kenyans sont inter-fertiles mais un seul de ces isolats, Ki3, a produit une descendance viable après croisement avec H. indica LN2. Les quatre isolats indonésiens sont interfertiles, mais un seul d'entre eux (INA H1) a produit des hybrides viables après croisement avec H. indica LN2. INA H1 a été également interfertile avec l'isolat kenyan Ki3.

Affiliations: 1: The Institute for Bioengineering and Agroecology, Department of Biology, National University of Ireland, Maynoothdare, Co. Kildaire, Ireland; 2: Sugarcane Breeding Institute, Indian Council for Agricultural Research, Coimbatore, Tamil Nadu 641 007, India; 3: The Institute for Bioengineering and Agroecology, Department of Biology, National University of Ireland, Maynooth, Co. Kildare, Ireland; 4: The Institute for Bioengineering and Agroecology, Department of Biology, National University of Ireland, Maynooth,


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