Cookies Policy

This site uses cookies. By continuing to browse the site you are agreeing to our use of cookies.

I accept this policy

Find out more here

Variations in ribosomal DNA sequences and phylogeny of Globodera parasitising solanaceous plants

No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
The full text of this article is not currently available.

Brill’s MyBook program is exclusively available on BrillOnline Books and Journals. Students and scholars affiliated with an institution that has purchased a Brill E-Book on the BrillOnline platform automatically have access to the MyBook option for the title(s) acquired by the Library. Brill MyBook is a print-on-demand paperback copy which is sold at a favorably uniform low price.

Access this article

+ Tax (if applicable)
Add to Favorites
You must be logged in to use this functionality

image of Nematology
For more content, see Nematologica.

The D3 expansion region of the 28S gene and the ITS1-5.8S-ITS2 region of rDNA sequences from Globodera rostochiensis, G. pallida, G. tabacum tabacum, G. tabacum virginiae and G. tabacum solanacearum have been aligned and compared. There are no nucleotide differences in the D3 region sequences between G. rostochiensis and G. pallida. Sequence analysis and RFLPs of ITS-PCR products showed that several haplotypes are present in the genomes of G. rostochiensis and G. pallida populations. Restriction patterns of PCR products for eight enzymes for differentiation of these two species are given. Phylogenetic analysis of 41 ITS region sequences obtained from populations and species of the subfamily Punctoderinae revealed four distinct main clades within Globodera parasitising solanaceous plants: G. rostochiensis, G. tabacum, G. pallida and an undescribed Globodera sp. from South America. The utility of RFLP profiles and sequences of the rDNA are discussed for diagnostics and phylogeny of Globodera .

Variabilité dans les séquences de l'ADN ribosomal et phylogénie des Globodera parasitant les Solanacées - La région d'expansion D3 du gène 28S et la région ITS1-5.8S-ITS2 des séquences d'ADNr de Globodera rostochiensis, G. pallida, G. tabacum tabacum, G. tabacum virginiae et G. tabacum solanacearum ont été alignées et comparées. Il n'y a pas de différence dans les nucléotides de la séquence de la région D3 entre G.rostochiensis et G.pallida. L'analyse séquentielle et les RFLP des produits du ITS-PCR montrent que plusieurs haplotypes sont présents dans les génomes des populations de G. rostochiensis et G. pallida. Les profils de restriction des produits du PCR de huit enzymes choisies pour la différenciation de ces deux espèces sont donnés. L'analyse phylogénique séquentielle de 41 regions ITS de populations et espèces appartenant à la sous-famille des Punctonerinae a révélé l'existence de quatre clades chez les Globodera parasitant les Solanacées: G. rostochiensis, G. tabacum, G. pallida et une espèce non décrite provenant d'Amérique du Sud. L'utilité pour le diagnostic et la phylogénie des Globodera des profils de RFLP et des séquences d'ADNr est discutée.

Affiliations: 1: Institute of Parasitology of Russian Academy of Sciences, Leninskii prospect 33, Moscow, 117071, Russia; 2: Entomology and Nematology Department, IACR-Rothamsted, Harpenden, Herfordshire AL5 JQ, UK


Full text loading...


Data & Media loading...

Article metrics loading...



Can't access your account?
  • Tools

  • Add to Favorites
  • Printable version
  • Email this page
  • Subscribe to ToC alert
  • Get permissions
  • Recommend to your library

    You must fill out fields marked with: *

    Librarian details
    Your details
    Why are you recommending this title?
    Select reason:
    Nematology — Recommend this title to your library
  • Export citations
  • Key

  • Full access
  • Open Access
  • Partial/No accessInformation