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SXP/RAL-2 proteins of the potato cyst nematode Globodera rostochiensis: secreted proteins of the hypodermis and amphids

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A family of secreted proteins (the SXP/RAL-2 proteins) has been identified in animal parasitic nematodes and Caenorhabditis elegans. In this paper, we describe two full length cDNA sequences from the potato cyst nematode Globodera rostochiensis which could encode proteins similar to SXP/ RAL-2 proteins. We show that although both genes are expressed in second stage juveniles and in sedentary females of G. rostochiensis, they have remarkably different spatial expression patterns. The mRNA derived from one of the genes (gr-sxp-1) was present in the hypodermis, as has been described previously for one of these genes in an animal parasitic nematode. However, expression of the mRNA from the other gene was restricted to the gland cells surrounding the amphidial sense organs (the sheath cells). In addition to providing the first description of SXP proteins from a plant parasitic nematode, this work provides the first detailed characterisation of a protein secreted from the amphids of a plant parasite.

Les protéines SXP/ RAL-2 du nématode à kyste de la pomme de terre Globodera rostochiensis : les protéines sécrétées par l'hypoderme et les amphides - Une famille de protéines sécrétées - les protéines SXP/RAL-2 - avait déjà été identifiée chez les nématodes zooparasites et chez Caenorhabditis elegans. Dans cet article nous décrivons deux séquences en longueur totale de cADN provenant du nématode à kyste de la pomme de terre Globodera rostochiensis, séquences qui pourraient coder des protéines similaires aux protéines SXP/RAL-2. Nous montrons que, bien que l'un et l'autre gène s'expriment chez les juvéniles de deuxième stade et chez les femelles sédentaires de Globodera rostochiensis, ils possèdent des profils d'expression spatiale remarquablement différents. Le rARN dérivé à partir d'un de ces gènes (gr-sxp-1) était présent dants l'hypoderme, comme cela avait déjà été décrit pour l'un de ces gènes chez un nématode zooparasite. Cependant, l'expression du mRNA de l'autre gène était limitée aux cellules glandulaires entourant les organes sensoriels amphidiens (les cellules de la gaine). Offrant la première description de protéines SXP provenant de nématodes phytopathogènes, le présent travail fournit de plus la première caractérisation détaillée d'une protéine sécrétée par les amphides d'un nématode parasite de plantes.

Affiliations: 1: Mycology, Bacteriology and Nematology Unit, Scottish Crop Research Institute, Invergowrie, Dundee, DD2 5DA, UK; 2: Wageningen University, Laboratory of Nematology, Binnenhaven 10, 6709 PD Wageningen, The Netherlands

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/content/journals/10.1163/156854100750112833
2000-11-01
2016-12-08

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