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Partial isolation of a water soluble pheromone from the sugar beet cyst nematode, Heterodera schachtii, using a novel bioassay

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image of Nematology
For more content, see Nematologica.

The sex pheromone of female Heterodera schachtii, the sugar beet cyst nematode, was partially isolated using high performance liquid chromatography (HPLC). Female-conditioned medium (FCM), produced by incubating 20 H. schachtii females in 1 ml of ultrapure water, attracted males in a concentration dependent manner. The attractive material was not contained within the female gelatinous matrix and did not attract second stage juveniles (J2). Characterisation of FCM revealed that the attractive material was water soluble and heat stable. Male H. schachtii exhibited stylet thrusting behaviour upon exposure to FCM, and this behaviour was used as a novel indicator of biological activity.

Affiliations: 1: Department of Biological Sciences, Brock University, St. Catharines, ON, Canada, L2S 2AI; 2: Agriculture and Agri-Food Canada, SCPFRC, London, ON, Canada, N5V 4T3; 3: Agriculture and Agri-Food Canada, SCPFRC, Vinland Station, ON, Canada, L0R 2EO


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