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A rapid method for the identification of the soybean cyst nematode Heterodera glycines using duplex PCR

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image of Nematology
For more content, see Nematologica.

A method for rapid identification of juveniles and cysts of the soybean cyst nematode based on PCR with species specific primers is described. The PCR assay was tested on 53 populations originating from China, Russia, USA and Brazil. A single cyst or second stage juvenile of Heterodera glycines alone or in a mixture with other soil inhabiting nematodes was detectable.

Affiliations: 1: Institute of Parasitology of Russian Academy of Sciences, Leninskii prospect 33, Moscow, 117071, Russia; 2: Institute of Plant Protection of Chinese Academy of Agricultural Sciences, Beijing 100094, China; 3: Agricultural Research Centre, Crop Protection Department, Burg. Van Gansberghelaan 96, 9820 Merelbeke, Belgium


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